Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
International Journal of Molecular Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1107-3756 Online ISSN: 1791-244X
Journal Cover
February-2017 Volume 39 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
February-2017 Volume 39 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Inhibitory effects of alpha-lipoic acid on oxidative stress in the rostral ventrolateral medulla in rats with salt-induced hypertension

  • Authors:
    • Yu‑Peng Huang
    • Hong‑Yan Jin
    • Hui‑Ping Yu
  • View Affiliations / Copyright

    Affiliations: Department of Cardiovascular Medicine, Hanyang Hospital of Wuhan, Wuhan, Hubei 430050, P.R. China
  • Pages: 430-436
    |
    Published online on: December 30, 2016
       https://doi.org/10.3892/ijmm.2016.2846
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Oxidative stress in the rostral ventrolateral medulla (RVLM) plays an important role in the pathophysiology of hypertension. Alpha‑lipoic acid (ALA) is widely recognized for its potent superoxide inhibitory properties, and it can safely penetrate deep into the brain. The aim of this study was to explore whether ALA supplementation attenuates hypertensive responses and cardiac hypertrophy by decreasing the NAD(P)H oxidase (NOX)-derived overproduction of reactive oxygen species (ROS) in the mitochondria in the RVLM, and thus attenuating the development of salt‑induced hypertension. For this purpose, male Wistar rats were randomly divided into 2 groups and either fed a high-salt diet or not. After 8 weeks, the rats were either administered ALA or an equal volume of the vehicle for 8 weeks. The rats fed a high‑salt diet exhibited higher mean arterial pressure (MAP) and higher plasma noradrenaline (NE) levels, as well as cardiac hypertrophy, as evidence by the increased whole heart weight/body weight (WHW/BW) ratio, WHW/tibia length (TL) ratio and left‑ventricular weight (LVW)/TL ratio. Compared with the rats in the NS group, the rats in the HS group only exhibited increased levels of superoxide, NOX2, NOX4 and mitochondrial malondialdehyde (MDA), but also decreased levels of copper/zinc (Cu/Zn)-superoxide dismutase (SOD), mitochondrial SOD and glutathione (GSH) in the RVLM. The supplementation of ALA decreased MAP, plasma NE levels and the levels of cardiac hypertrophy indicators. It also decreased the levels of superoxide, NOX2, NOX4 and mitochondrial MDA, and increased the levels of Cu/Zn‑SOD, mitochondrial SOD and GSH in the RVLM compared with the rats fed a high-salt diet and not treated with ALA. On the whole, our findings indicate that long‑term ALA supplementation attenuates hypertensive responses and cardiac hypertrophy by decreasing the expression of NAD(P)H subunits (NOX2 and NOX4), increasing the levels of mitochondrial bioenergetic enzymes, and enhancing the intracellular antioxidant capacity in the RVLM during the development of hypertension.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

Figure 7

View References

1 

Hall JE, Granger JP, do Carmo JM, da Silva AA, Dubinion J, George E, Hamza S, Speed J and Hall ME: Hypertension: Physiology and pathophysiology. Compr Physiol. 2:2393–2442. 2012.

2 

Montezano AC, Dulak-Lis M, Tsiropoulou S, Harvey A, Briones AM and Touyz RM: Oxidative stress and human hypertension: Vascular mechanisms, biomarkers, and novel therapies. Can J Cardiol. 31:631–641. 2015. View Article : Google Scholar : PubMed/NCBI

3 

Virdis A, Bacca A, Colucci R, Duranti E, Fornai M, Materazzi G, Ippolito C, Bernardini N, Blandizzi C, Bernini G, et al: Endothelial dysfunction in small arteries of essential hypertensive patients: Role of cyclooxygenase-2 in oxidative stress generation. Hypertension. 62:337–344. 2013. View Article : Google Scholar : PubMed/NCBI

4 

Vanhoutte PM, Shimokawa H, Tang EH and Feletou M: Endothelial dysfunction and vascular disease. Acta Physiol (Oxf). 196:193–222. 2009. View Article : Google Scholar

5 

Koga Y, Hirooka Y, Araki S, Nozoe M, Kishi T and Sunagawa K: High salt intake enhances blood pressure increase during development of hypertension via oxidative stress in rostral ventrolateral medulla of spontaneously hypertensive rats. Hypertens Res. 31:2075–2083. 2008. View Article : Google Scholar : PubMed/NCBI

6 

Liu YZ, Chen JK, Li ZP, Zhao T, Ni M, Li DJ, Jiang CL and Shen FM: High-salt diet enhances hippocampal oxidative stress and cognitive impairment in mice. Neurobiol Learn Mem. 114:10–15. 2014. View Article : Google Scholar : PubMed/NCBI

7 

Higashi Y, Maruhashi T, Noma K and Kihara Y: Oxidative stress and endothelial dysfunction: Clinical evidence and therapeutic implications. Trends Cardiovasc Med. 24:165–169. 2014. View Article : Google Scholar : PubMed/NCBI

8 

Andrades ME, Ritter C and Dal-Pizzol F: The role of free radicals in sepsis development. Front Biosci (Elite Ed). 1:277–287. 2009.

9 

Barichello T, Fortunato JJ, Vitali AM, Feier G, Reinke A, Moreira JC, Quevedo J and Dal-Pizzol F: Oxidative variables in the rat brain after sepsis induced by cecal ligation and perforation. Crit Care Med. 34:886–889. 2006. View Article : Google Scholar : PubMed/NCBI

10 

Zhang ZH, Wei SG, Francis J and Felder RB: Cardiovascular and renal sympathetic activation by blood-borne TNF-alpha in rat: The role of central prostaglandins. Am J Physiol Regul Integr Comp Physiol. 284:R916–R927. 2003. View Article : Google Scholar : PubMed/NCBI

11 

Kumagai H, Oshima N, Matsuura T, Iigaya K, Imai M, Onimaru H, Sakata K, Osaka M, Onami T, Takimoto C, et al: Importance of rostral ventrolateral medulla neurons in determining efferent sympathetic nerve activity and blood pressure. Hypertens Res. 35:132–141. 2012. View Article : Google Scholar :

12 

Brooks VL, Haywood JR and Johnson AK: Translation of salt retention to central activation of the sympathetic nervous system in hypertension. Clin Exp Pharmacol Physiol. 32:426–432. 2005. View Article : Google Scholar : PubMed/NCBI

13 

Carlson SH, Roysomutti S, Peng N and Wyss JM: The role of the central nervous system in NaCl-sensitive hypertension in spontaneously hypertensive rats. Am J Hypertens. 14:155S–162S. 2001. View Article : Google Scholar : PubMed/NCBI

14 

Smith AR, Shenvi SV, Widlansky M, Suh JH and Hagen TM: Lipoic acid as a potential therapy for chronic diseases associated with oxidative stress. Curr Med Chem. 11:1135–1146. 2004. View Article : Google Scholar : PubMed/NCBI

15 

McNeilly AM, Davison GW, Murphy MH, Nadeem N, Trinick T, Duly E, Novials A and McEneny J: Effect of α-lipoic acid and exercise training on cardiovascular disease risk in obesity with impaired glucose tolerance. Lipids Health Dis. 10:2172011. View Article : Google Scholar

16 

Wollin SD and Jones PJ: Alpha-lipoic acid and cardiovascular disease. J Nutr. 133:3327–3330. 2003.PubMed/NCBI

17 

Petronilho F, Florentino D, Danielski LG, Vieira LC, Martins M, Vieira A, Bonfante S, Goldim MP and Vuolo F: Alpha-Lipoic Acid Attenuates Oxidative Damage in Organs After Sepsis. Inflammation. 39:357–365. 2016. View Article : Google Scholar

18 

Elks CM, Reed SD, Mariappan N, Shukitt-Hale B, Joseph JA, Ingram DK and Francis J: A blueberry-enriched diet attenuates nephropathy in a rat model of hypertension via reduction in oxidative stress. PLoS One. 6:e240282011. View Article : Google Scholar : PubMed/NCBI

19 

Gao L, Wang W, Li YL, Schultz HD, Liu D, Cornish KG and Zucker IH: Superoxide mediates sympathoexcitation in heart failure: roles of angiotensin II and NAD(P)H oxidase. Circ Res. 95:937–944. 2004. View Article : Google Scholar : PubMed/NCBI

20 

Su Q, Liu JJ, Cui W, Shi XL, Guo J, Li HB, Huo CJ, Miao YW, Zhang M, Yang Q, et al: Alpha lipoic acid supplementation attenuates reactive oxygen species in hypothalamic paraventricular nucleus and sympathoexcitation in high salt-induced hypertension. Toxicol Lett. 241:152–158. 2016. View Article : Google Scholar

21 

Agarwal D, Welsch MA, Keller JN and Francis J: Chronic exercise modulates RAS components and improves balance between pro- and anti-inflammatory cytokines in the brain of SHR. Basic Res Cardiol. 106:1069–1085. 2011. View Article : Google Scholar : PubMed/NCBI

22 

Lakroun Z, Kebieche M, Lahouel A, Zama D, Desor F and Soulimani R: Oxidative stress and brain mitochondria swelling induced by endosulfan and protective role of quercetin in rat. Environ. Sci Pollut Res Int. 22:7776–7781. 2015. View Article : Google Scholar

23 

Begue JA and Aust SD: Microsomal lipid peroxidation. Methods Enzymol. 52:302–310. 1978. View Article : Google Scholar

24 

Kono Y: Generation of superoxide radical during autoxidation of hydroxylamine and an assay for superoxide dismutase. Arch Biochem Biophys. 186:189–195. 1978. View Article : Google Scholar : PubMed/NCBI

25 

Sedlak J and Lindsay RH: Estimation of total protein bound and nonprotein sulfhydryl groups in tissue with Ellman's reagent. Anal Biochem. 25:192–205. 1968. View Article : Google Scholar : PubMed/NCBI

26 

Guggilam A, Cardinale JP, Mariappan N, Sriramula S, Haque M and Francis J: Central TNF inhibition results in attenuated neurohumoral excitation in heart failure: A role for superoxide and nitric oxide. Basic Res Cardiol. 106:273–286. 2011. View Article : Google Scholar : PubMed/NCBI

27 

Guggilam A, Haque M, Kerut EK, McIlwain E, Lucchesi P, Seghal I and Francis J: TNF-alpha blockade decreases oxidative stress in the paraventricular nucleus and attenuates sympathoex-citation in heart failure rats. Am J Physiol Heart Circ Physiol. 93:H599–H609. 2007. View Article : Google Scholar

28 

Karppanen H and Mervaala E: Sodium intake and hypertension. Prog Cardiovasc Dis. 49:59–75. 2006. View Article : Google Scholar : PubMed/NCBI

29 

Frohlich ED: The salt conundrum: A hypothesis. Hypertension. 50:161–166. 2007. View Article : Google Scholar : PubMed/NCBI

30 

Huang BS, Van Vliet BN and Leenen FH: Increases in CSF [Na+] precede the increases in blood pressure in Dahl S rats and SHR on a high-salt diet. Am J Physiol Heart Circ Physiol. 287:H1160–H1166. 2004. View Article : Google Scholar : PubMed/NCBI

31 

Huang BS, Cheung WJ, Wang H, Tan J, White RA and Leenen FH: Activation of brain renin-angiotensin-aldosterone system by central sodium in Wistar rats. Am J Physiol Heart Circ Physiol. 291:H1109–H1117. 2006. View Article : Google Scholar : PubMed/NCBI

32 

Huang BS, Amin MS and Leenen FH: The central role of the brain in salt-sensitive hypertension. Curr Opin Cardiol. 21:295–304. 2006. View Article : Google Scholar : PubMed/NCBI

33 

Wang H, Huang BS and Leenen FH: Brain sodium channels and ouabainlike compounds mediate central aldosterone-induced hypertension. Am J Physiol Heart Circ Physiol. 285:H2516–H2523. 2003. View Article : Google Scholar : PubMed/NCBI

34 

Nozoe M, Hirooka Y, Koga Y, Araki S, Konno S, Kishi T, Ide T and Sunagawa K: Mitochondria-derived reactive oxygen species mediate sympathoexcitation induced by angiotensin II in the rostral ventrolateral medulla. J Hypertens. 26:2176–2184. 2008. View Article : Google Scholar : PubMed/NCBI

35 

Zimmerman MC and Davisson RL: Redox signaling in central neural regulation of cardiovascular function. Prog Biophys Mol Biol. 84:125–149. 2004. View Article : Google Scholar : PubMed/NCBI

36 

Goraca A, Huk-Kolega H, Piechota A, Kleniewska P, Ciejka E and Skibska B: Lipoic acid - biological activity and therapeutic potential. Pharmacol Rep. 63:849–858. 2011. View Article : Google Scholar : PubMed/NCBI

37 

Abdel-Zaher AO, Abdel-Hady RH, Mahmoud MM and Farrag MM: The potential protective role of alpha-lipoic acid against acetaminophen-induced hepatic and renal damage. Toxicology. 243:261–270. 2008. View Article : Google Scholar

38 

Anto SK, Koyada N, Khan S and Jena G: α-Lipoic acid attenuates transplacental nicotine-induced germ cell and oxidative DNA damage in adult mice. J Basic Clin Physiol Pharmacol. 27:585–593. 2016. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Huang YP, Jin HY and Yu HP: Inhibitory effects of alpha-lipoic acid on oxidative stress in the rostral ventrolateral medulla in rats with salt-induced hypertension. Int J Mol Med 39: 430-436, 2017.
APA
Huang, Y., Jin, H., & Yu, H. (2017). Inhibitory effects of alpha-lipoic acid on oxidative stress in the rostral ventrolateral medulla in rats with salt-induced hypertension. International Journal of Molecular Medicine, 39, 430-436. https://doi.org/10.3892/ijmm.2016.2846
MLA
Huang, Y., Jin, H., Yu, H."Inhibitory effects of alpha-lipoic acid on oxidative stress in the rostral ventrolateral medulla in rats with salt-induced hypertension". International Journal of Molecular Medicine 39.2 (2017): 430-436.
Chicago
Huang, Y., Jin, H., Yu, H."Inhibitory effects of alpha-lipoic acid on oxidative stress in the rostral ventrolateral medulla in rats with salt-induced hypertension". International Journal of Molecular Medicine 39, no. 2 (2017): 430-436. https://doi.org/10.3892/ijmm.2016.2846
Copy and paste a formatted citation
x
Spandidos Publications style
Huang YP, Jin HY and Yu HP: Inhibitory effects of alpha-lipoic acid on oxidative stress in the rostral ventrolateral medulla in rats with salt-induced hypertension. Int J Mol Med 39: 430-436, 2017.
APA
Huang, Y., Jin, H., & Yu, H. (2017). Inhibitory effects of alpha-lipoic acid on oxidative stress in the rostral ventrolateral medulla in rats with salt-induced hypertension. International Journal of Molecular Medicine, 39, 430-436. https://doi.org/10.3892/ijmm.2016.2846
MLA
Huang, Y., Jin, H., Yu, H."Inhibitory effects of alpha-lipoic acid on oxidative stress in the rostral ventrolateral medulla in rats with salt-induced hypertension". International Journal of Molecular Medicine 39.2 (2017): 430-436.
Chicago
Huang, Y., Jin, H., Yu, H."Inhibitory effects of alpha-lipoic acid on oxidative stress in the rostral ventrolateral medulla in rats with salt-induced hypertension". International Journal of Molecular Medicine 39, no. 2 (2017): 430-436. https://doi.org/10.3892/ijmm.2016.2846
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team