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Article

High-resolution melting analysis for rapid and sensitive NOTCH1 screening in chronic lymphocytic leukemia

  • Authors:
    • Jing-Jing Xu
    • Fei-Rong Yao
    • Min Jiang
    • You-Tao Zhang
    • Feng Guo
  • View Affiliations / Copyright

    Affiliations: Center for Clinical Laboratory, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215006, P.R. China, Department of Radiology, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215006, P.R. China, Department of Blood Transfusion, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215006, P.R. China
  • Pages: 415-422
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    Published online on: January 5, 2017
       https://doi.org/10.3892/ijmm.2017.2849
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Abstract

Chronic lymphocytic leukemia (CLL) is a biological and clinical heterogeneous disease. Activating mutations of NOTCH1 have been implicated to be associated with adverse prognosis in CLL. The objective of the present study was to develop an effective high-resolution melting (HRM) assay for detecting NOTCH1 mutations. Genomic DNA (gDNA) extracted from 133 CLL patients was screened by HRM assay, and the results were compared with the data obtained using direct sequencing. The relative sensitivity of the HRM assay and direct sequencing was evaluated using diluted gDNA with different NOTCH1 mutational frequencies. The HRM assay was able to detect and discriminate samples with NOTCH1 mutations from the wild-type template in CLL. Eight of the 133 CLL patients (6.02%) were scored positively for NOTCH1 mutations in the HRM assay. The results of the NOTCH1 mutations detected by HRM analysis achieved 100% concordance with those determined from direct sequencing. HRM had a higher sensitivity (1%) and shorter turn-around time (TAT), compared to direct sequencing. In conclusion, the HRM assay developed by us was confirmed to be a rapid, sensitive, and promising approach for high-throughput prognostic NOTCH1 screening in CLL. It enables real-time NOTCH1 evaluation, which is of great significance in clinical practice and may facilitate the decision-making of clinicians in CLL.
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1 

Zenz T, Mertens D, Küppers R, Döhner H and Stilgenbauer S: From pathogenesis to treatment of chronic lymphocytic leukaemia. Nat Rev Cancer. 10:37–50. 2010.

2 

Gaidano G, Foà R and Dalla-Favera R: Molecular pathogenesis of chronic lymphocytic leukemia. J Clin Invest. 122:3432–3438. 2012. View Article : Google Scholar : PubMed/NCBI

3 

Chiorazzi N, Rai KR and Ferrarini M: Chronic lymphocytic leukemia. N Engl J Med. 352:804–815. 2005. View Article : Google Scholar : PubMed/NCBI

4 

Tam CS and Keating MJ: Chemoimmunotherapy of chronic lymphocytic leukemia. Nat Rev Clin Oncol. 7:521–532. 2010. View Article : Google Scholar : PubMed/NCBI

5 

Puente XS, Pinyol M, Quesada V, Conde L, Ordóñez GR, Villamor N, Escaramis G, Jares P, Beà S, González-Díaz M, et al: Whole-genome sequencing identifies recurrent mutations in chronic lymphocytic leukaemia. Nature. 475:101–105. 2011. View Article : Google Scholar : PubMed/NCBI

6 

Rossi D, Fangazio M, Rasi S, Vaisitti T, Monti S, Cresta S, Chiaretti S, Del Giudice I, Fabbri G, Bruscaggin A, et al: Disruption of BIRC3 associates with fludarabine chemore-fractoriness in TP53 wild-type chronic lymphocytic leukemia. Blood. 119:2854–2862. 2012. View Article : Google Scholar : PubMed/NCBI

7 

Blaumueller CM, Qi H, Zagouras P and Artavanis-Tsakonas S: Intracellular cleavage of Notch leads to a heterodimeric receptor on the plasma membrane. Cell. 90:281–291. 1997. View Article : Google Scholar : PubMed/NCBI

8 

Yuan JS, Kousis PC, Suliman S, Visan I and Guidos CJ: Functions of notch signaling in the immune system: Consensus and controversies. Annu Rev Immunol. 28:343–365. 2010. View Article : Google Scholar : PubMed/NCBI

9 

Lobry C, Oh P and Aifantis I: Oncogenic and tumor suppressor functions of Notch in cancer: It's NOTCH what you think. J Exp Med. 208:1931–1935. 2011. View Article : Google Scholar : PubMed/NCBI

10 

Lobry C, Oh P, Mansour MR, Look AT and Aifantis I: Notch signaling: Switching an oncogene to a tumor suppressor. Blood. 123:2451–2459. 2014. View Article : Google Scholar : PubMed/NCBI

11 

Rosati E, Sabatini R, Rampino G, Tabilio A, Di Ianni M, Fettucciari K, Bartoli A, Coaccioli S, Screpanti I and Marconi P: Constitutively activated Notch signaling is involved in survival and apoptosis resistance of B-CLL cells. Blood. 113:856–865. 2009. View Article : Google Scholar

12 

Arruga F, Gizdic B, Serra S, Vaisitti T, Ciardullo C, Coscia M, Laurenti L, D'Arena G, Jaksic O, Inghirami G, et al: Functional impact of NOTCH1 mutations in chronic lymphocytic leukemia. Leukemia. 28:1060–1070. 2014. View Article : Google Scholar

13 

Fabbri G, Rasi S, Rossi D, Trifonov V, Khiabanian H, Ma J, Grunn A, Fangazio M, Capello D, Monti S, et al: Analysis of the chronic lymphocytic leukemia coding genome: Role of NOTCH1 mutational activation. J Exp Med. 208:1389–1401. 2011. View Article : Google Scholar : PubMed/NCBI

14 

Rossi D, Rasi S, Fabbri G, Spina V, Fangazio M, Forconi F, Marasca R, Laurenti L, Bruscaggin A, Cerri M, et al: Mutations of NOTCH1 are an independent predictor of survival in chronic lymphocytic leukemia. Blood. 119:521–529. 2012. View Article : Google Scholar :

15 

Weissmann S, Roller A, Jeromin S, Hernández M, Abáigar M, Hernández-Rivas JM, Grossmann V, Haferlach C, Kern W, Haferlach T, et al: Prognostic impact and landscape of NOTCH1 mutations in chronic lymphocytic leukemia (CLL): A study on 852 patients. Leukemia. 27:2393–2396. 2013. View Article : Google Scholar : PubMed/NCBI

16 

López-Guerra M, Xargay-Torrent S, Rosich L, Montraveta A, Roldán J, Matas-Céspedes A, Villamor N, Aymerich M, López-Otín C, Pérez-Galán P, et al: The γ-secretase inhibitor PF-03084014 combined with fludarabine antagonizes migration, invasion and angiogenesis in NOTCH1-mutated CLL cells. Leukemia. 29:96–106. 2015. View Article : Google Scholar

17 

Osipo C, Golde TE, Osborne BA and Miele LA: Off the beaten pathway: The complex cross talk between Notch and NF-kappaB. Lab Invest. 11–17. 2008. View Article : Google Scholar

18 

Di Ianni M, Baldoni S, Rosati E, Ciurnelli R, Cavalli L, Martelli MF, Marconi P, Screpanti I and Falzetti F: A new genetic lesion in B-CLL: A NOTCH1 PEST domain mutation. Br J Haematol. 146:689–691. 2009. View Article : Google Scholar : PubMed/NCBI

19 

Sportoletti P, Baldoni S, Cavalli L, Del Papa B, Bonifacio E, Ciurnelli R, Bell AS, Di Tommaso A, Rosati E, Crescenzi B, et al: NOTCH1 PEST domain mutation is an adverse prognostic factor in B-CLL. Br J Haematol. 151:404–406. 2010. View Article : Google Scholar : PubMed/NCBI

20 

Sportoletti P, Baldoni S, Del Papa B, Aureli P, Dorillo E, Ruggeri L, Plebani S, Amico V, Di Tommaso A, Rosati E, et al: A revised NOTCH1 mutation frequency still impacts survival while the allele burden predicts early progression in chronic lymphocytic leukemia. Leukemia. 28:436–439. 2014. View Article : Google Scholar

21 

Stilgenbauer S, Schnaiter A, Paschka P, Zenz T, Rossi M, Döhner K, Bühler A, Böttcher S, Ritgen M, Kneba M, et al: Gene mutations and treatment outcome in chronic lymphocytic leukemia: Results from the CLL8 trial. Blood. 123:3247–3254. 2014. View Article : Google Scholar : PubMed/NCBI

22 

Oscier DG, Rose-Zerilli MJ, Winkelmann N, Gonzalez de Castro D, Gomez B, Forster J, Parker H, Parker A, Gardiner A, Collins A, et al: The clinical significance of NOTCH1 and SF3B1 mutations in the UK LRF CLL4 trial. Blood. 121:468–475. 2013. View Article : Google Scholar

23 

Puente XS, Beà S, Valdés-Mas R, Villamor N, Gutiérrez-Abril J, Martín-Subero JI, Munar M, Rubio-Pérez C, Jares P, Aymerich M, et al: Non-coding recurrent mutations in chronic lymphocytic leukaemia. Nature. 526:519–524. 2015. View Article : Google Scholar : PubMed/NCBI

24 

Gundry CN, Vandersteen JG, Reed GH, Pryor RJ, Chen J and Wittwer CT: Amplicon melting analysis with labeled primers: A closed-tube method for differentiating homozygotes and hetero-zygotes. Clin Chem. 49:396–406. 2003. View Article : Google Scholar : PubMed/NCBI

25 

Reed GH, Kent JO and Wittwer CT: High-resolution DNA melting analysis for simple and efficient molecular diagnostics. Pharmacogenomics. 8:597–608. 2007. View Article : Google Scholar : PubMed/NCBI

26 

Taylor CF: Mutation scanning using high-resolution melting. Biochem Soc Trans. 37:433–437. 2009. View Article : Google Scholar : PubMed/NCBI

27 

Liew M, Pryor R, Palais R, Meadows C, Erali M, Lyon E and Wittwer C: Genotyping of single-nucleotide polymorphisms by high-resolution melting of small amplicons. Clin Chem. 50:1156–1164. 2004. View Article : Google Scholar : PubMed/NCBI

28 

Reed GH and Wittwer CT: Sensitivity and specificity of single-nucleotide polymorphism scanning by high-resolution melting analysis. Clin Chem. 50:1748–1754. 2004. View Article : Google Scholar : PubMed/NCBI

29 

Graham R, Liew M, Meadows C, Lyon E and Wittwer CT: Distinguishing different DNA heterozygotes by high-resolution melting. Clin Chem. 51:1295–1298. 2005. View Article : Google Scholar : PubMed/NCBI

30 

Herrmann MG, Durtschi JD, Bromley LK, Wittwer CT and Voelkerding KV: Amplicon DNA melting analysis for mutation scanning and genotyping: Cross-platform comparison of instruments and dyes. Clin Chem. 52:494–503. 2006. View Article : Google Scholar : PubMed/NCBI

31 

Montgomery J, Wittwer CT, Palais R and Zhou L: Simultaneous mutation scanning and genotyping by high-resolution DNA melting analysis. Nat Protoc. 2:59–66. 2007. View Article : Google Scholar : PubMed/NCBI

32 

Wei P, Walls M, Qiu M, Ding R, Denlinger RH, Wong A, Tsaparikos K, Jani JP, Hosea N, Sands M, et al: Evaluation of selective gamma-secretase inhibitor PF-03084014 for its antitumor efficacy and gastrointestinal safety to guide optimal clinical trial design. Mol Cancer Ther. 9:1618–1628. 2010. View Article : Google Scholar : PubMed/NCBI

33 

Wu Y, Cain-Hom C, Choy L, Hagenbeek TJ, de Leon GP, Chen Y, Finkle D, Venook R, Wu X, Ridgway J, et al: Therapeutic antibody targeting of individual Notch receptors. Nature. 464:1052–1057. 2010. View Article : Google Scholar : PubMed/NCBI

34 

Schmidt U, Hulkkonen J and Naue J: Detection of a G>C single nucleotide polymorphism within a repetitive DNA sequence by high-resolution DNA melting. Int J Legal Med. Mar 14–2016.Epub ahead of print. View Article : Google Scholar

35 

Hong Y, Pandey MK, Liu Y, Chen X, Liu H, Varshney RK, Liang X and Huang S: Identification and evaluation of single-nucleotide polymorphisms in allotetraploid peanut (Arachis hypogaea L.) based on amplicon sequencing combined with high resolution melting (HRM) analysis. Front Plant Sci. 6:10682015. View Article : Google Scholar : PubMed/NCBI

36 

de Juan I, Esteban E, Palanca S, Barragán E and Bolufer P: High-resolution melting analysis for rapid screening of BRCA1 and BRCA2 Spanish mutations. Breast Cancer Res Treat. 115:405–414. 2009. View Article : Google Scholar

37 

Er TK and Chang JG: High-resolution melting: Applications in genetic disorders. Clin Chim Acta. 414:197–201. 2012. View Article : Google Scholar : PubMed/NCBI

38 

Xia Y, Fan L, Wang L, Gale RP, Wang M, Tian T, Wu W, Yu L, Chen YY, Xu W, et al: Frequencies of SF3B1, NOTCH1, MYD88, BIRC3 and IGHV mutations and TP53 disruptions in Chinese with chronic lymphocytic leukemia: Disparities with Europeans. Oncotarget. 6:5426–5434. 2015. View Article : Google Scholar : PubMed/NCBI

39 

Shedden K, Li Y, Ouillette P and Malek SN: Characteristics of chronic lymphocytic leukemia with somatically acquired mutations in NOTCH1 exon 34. Leukemia. 26:1108–1110. 2012. View Article : Google Scholar

40 

Balatti V, Bottoni A, Palamarchuk A, Alder H, Rassenti LZ, Kipps TJ, Pekarsky Y and Croce CM: NOTCH1 mutations in CLL associated with trisomy 12. Blood. 119:329–331. 2012. View Article : Google Scholar :

41 

Del Giudice I, Rossi D, Chiaretti S, Marinelli M, Tavolaro S, Gabrielli S, Laurenti L, Marasca R, Rasi S, Fangazio M, et al: NOTCH1 mutations in +12 chronic lymphocytic leukemia (CLL) confer an unfavorable prognosis, induce a distinctive transcriptional profiling and refine the intermediate prognosis of +12 CLL. Haematologica. 97:437–441. 2012. View Article : Google Scholar :

42 

Villamor N, Conde L, Martínez-Trillos A, Cazorla M, Navarro A, Beà S, López C, Colomer D, Pinyol M, Aymerich M, et al: NOTCH1 mutations identify a genetic subgroup of chronic lymphocytic leukemia patients with high risk of transformation and poor outcome. Leukemia. 27:1100–1106. 2013. View Article : Google Scholar : PubMed/NCBI

43 

Do H, Krypuy M, Mitchell PL, Fox SB and Dobrovic A: High resolution melting analysis for rapid and sensitive EGFR and KRAS mutation detection in formalin fixed paraffin embedded biopsies. BMC Cancer. 8:1422008. View Article : Google Scholar : PubMed/NCBI

44 

Marino M, Monzani ML, Brigante G, Cioni K, Madeo B, Santi D, Maiorana A, Bettelli S, Moriondo V, Pignatti E, et al: High-resolution melting is a sensitive, cost-effective, time-saving technique for BRAF V600E detection in thyroid FNAB washing liquid: A prospective cohort study. Eur Thyroid J. 4:73–81. 2015. View Article : Google Scholar : PubMed/NCBI

45 

Wright GM, Do H, Weiss J, Alam NZ, Rathi V, Walkiewicz M, John T, Russell PA and Dobrovic A: Mapping of actionable mutations to histological subtype domains in lung adenocarcinoma: Implications for precision medicine. Oncotarget. 5:2107–2115. 2014. View Article : Google Scholar : PubMed/NCBI

46 

El Khachibi M, Diakite B, Hamzi K, Badou A, Senhaji MA, Bakhchane A, Jouhadi H, Barakat A, Benider A and Nadifi S: Screening of exon 11 of BRCA1 gene using the high resolution melting approach for diagnosis in Moroccan breast cancer patients. BMC Cancer. 15:812015. View Article : Google Scholar : PubMed/NCBI

47 

Wang J, Ren X, Bai X, Zhang T, Wang Y, Li K and Li G: Identification of gene mutation in patients with osteogenesis imperfecta using high resolution melting analysis. Sci Rep. 5:134682015. View Article : Google Scholar

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Copy and paste a formatted citation
Spandidos Publications style
Xu J, Yao F, Jiang M, Zhang Y and Guo F: High-resolution melting analysis for rapid and sensitive NOTCH1 screening in chronic lymphocytic leukemia. Int J Mol Med 39: 415-422, 2017.
APA
Xu, J., Yao, F., Jiang, M., Zhang, Y., & Guo, F. (2017). High-resolution melting analysis for rapid and sensitive NOTCH1 screening in chronic lymphocytic leukemia. International Journal of Molecular Medicine, 39, 415-422. https://doi.org/10.3892/ijmm.2017.2849
MLA
Xu, J., Yao, F., Jiang, M., Zhang, Y., Guo, F."High-resolution melting analysis for rapid and sensitive NOTCH1 screening in chronic lymphocytic leukemia". International Journal of Molecular Medicine 39.2 (2017): 415-422.
Chicago
Xu, J., Yao, F., Jiang, M., Zhang, Y., Guo, F."High-resolution melting analysis for rapid and sensitive NOTCH1 screening in chronic lymphocytic leukemia". International Journal of Molecular Medicine 39, no. 2 (2017): 415-422. https://doi.org/10.3892/ijmm.2017.2849
Copy and paste a formatted citation
x
Spandidos Publications style
Xu J, Yao F, Jiang M, Zhang Y and Guo F: High-resolution melting analysis for rapid and sensitive NOTCH1 screening in chronic lymphocytic leukemia. Int J Mol Med 39: 415-422, 2017.
APA
Xu, J., Yao, F., Jiang, M., Zhang, Y., & Guo, F. (2017). High-resolution melting analysis for rapid and sensitive NOTCH1 screening in chronic lymphocytic leukemia. International Journal of Molecular Medicine, 39, 415-422. https://doi.org/10.3892/ijmm.2017.2849
MLA
Xu, J., Yao, F., Jiang, M., Zhang, Y., Guo, F."High-resolution melting analysis for rapid and sensitive NOTCH1 screening in chronic lymphocytic leukemia". International Journal of Molecular Medicine 39.2 (2017): 415-422.
Chicago
Xu, J., Yao, F., Jiang, M., Zhang, Y., Guo, F."High-resolution melting analysis for rapid and sensitive NOTCH1 screening in chronic lymphocytic leukemia". International Journal of Molecular Medicine 39, no. 2 (2017): 415-422. https://doi.org/10.3892/ijmm.2017.2849
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