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Article

Phytosphingosine-1-phosphate and epidermal growth factor synergistically restore extracellular matrix in human dermal fibroblasts in vitro and in vivo

  • Authors:
    • Seung Bin Kwon
    • Sungkwan An
    • Min Jung Kim
    • Ka Ram Kim
    • Young Min Choi
    • Kyu Joong Ahn
    • In-Sook An
    • Hwa Jun Cha
  • View Affiliations / Copyright

    Affiliations: KISCS Incorporated, Cheongju-si, Chungcheongbuk-do 361-951, Republic of Korea, Korea Institute for Skin and Clinical Sciences, Konkuk University, Seoul 143-701, Republic of Korea, Department of Dermatology, Konkuk University School of Medicine, Seoul 143-701, Republic of Korea
  • Pages: 741-748
    |
    Published online on: January 23, 2017
       https://doi.org/10.3892/ijmm.2017.2866
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Abstract

Phytosphingosine-1-phosphate (PhS1P), which is found in plants and fungi, is generated by the phosphorylation of phytosphingosine and is structurally similar to molecules that promote cellular growth and proliferation. The aim of this study was to ascertain whether PhS1P displays synergistic effects together with epidermal growth factor (EGF), which is also critical for activating proliferation, migration and survival pathways. We utilized cultured human dermal fibroblasts (HDFs) and a number of assays, including western blotting, cell migration assays, quantitative (real-time) PCR, and viability assays. We found that PhS1P promoted the activity of EGF in vitro. We then conducted a clinical trial in females over 35 years of age, with visible signs of skin aging. By evaluating skin hydration, dermal density and thickness, length of fine wrinkles, and skin elasticity, we verified the clinical efficacy of a combined treatment of PhS1P and EGF in vivo. On the whole, our data suggest that PhS1P displays a synergistic anti-aging effect together with EGF, both in vitro and in vivo.
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Copy and paste a formatted citation
Spandidos Publications style
Kwon SB, An S, Kim MJ, Kim KR, Choi YM, Ahn KJ, An I and Cha HJ: Phytosphingosine-1-phosphate and epidermal growth factor synergistically restore extracellular matrix in human dermal fibroblasts in vitro and in vivo. Int J Mol Med 39: 741-748, 2017.
APA
Kwon, S.B., An, S., Kim, M.J., Kim, K.R., Choi, Y.M., Ahn, K.J. ... Cha, H.J. (2017). Phytosphingosine-1-phosphate and epidermal growth factor synergistically restore extracellular matrix in human dermal fibroblasts in vitro and in vivo. International Journal of Molecular Medicine, 39, 741-748. https://doi.org/10.3892/ijmm.2017.2866
MLA
Kwon, S. B., An, S., Kim, M. J., Kim, K. R., Choi, Y. M., Ahn, K. J., An, I., Cha, H. J."Phytosphingosine-1-phosphate and epidermal growth factor synergistically restore extracellular matrix in human dermal fibroblasts in vitro and in vivo". International Journal of Molecular Medicine 39.3 (2017): 741-748.
Chicago
Kwon, S. B., An, S., Kim, M. J., Kim, K. R., Choi, Y. M., Ahn, K. J., An, I., Cha, H. J."Phytosphingosine-1-phosphate and epidermal growth factor synergistically restore extracellular matrix in human dermal fibroblasts in vitro and in vivo". International Journal of Molecular Medicine 39, no. 3 (2017): 741-748. https://doi.org/10.3892/ijmm.2017.2866
Copy and paste a formatted citation
x
Spandidos Publications style
Kwon SB, An S, Kim MJ, Kim KR, Choi YM, Ahn KJ, An I and Cha HJ: Phytosphingosine-1-phosphate and epidermal growth factor synergistically restore extracellular matrix in human dermal fibroblasts in vitro and in vivo. Int J Mol Med 39: 741-748, 2017.
APA
Kwon, S.B., An, S., Kim, M.J., Kim, K.R., Choi, Y.M., Ahn, K.J. ... Cha, H.J. (2017). Phytosphingosine-1-phosphate and epidermal growth factor synergistically restore extracellular matrix in human dermal fibroblasts in vitro and in vivo. International Journal of Molecular Medicine, 39, 741-748. https://doi.org/10.3892/ijmm.2017.2866
MLA
Kwon, S. B., An, S., Kim, M. J., Kim, K. R., Choi, Y. M., Ahn, K. J., An, I., Cha, H. J."Phytosphingosine-1-phosphate and epidermal growth factor synergistically restore extracellular matrix in human dermal fibroblasts in vitro and in vivo". International Journal of Molecular Medicine 39.3 (2017): 741-748.
Chicago
Kwon, S. B., An, S., Kim, M. J., Kim, K. R., Choi, Y. M., Ahn, K. J., An, I., Cha, H. J."Phytosphingosine-1-phosphate and epidermal growth factor synergistically restore extracellular matrix in human dermal fibroblasts in vitro and in vivo". International Journal of Molecular Medicine 39, no. 3 (2017): 741-748. https://doi.org/10.3892/ijmm.2017.2866
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