Phytosphingosine-1-phosphate and epidermal growth factor synergistically restore extracellular matrix in human dermal fibroblasts in vitro and in vivo

Kwon,Seung  Bin; An,Sungkwan; Kim,Min  Jung; Kim,Ka  Ram; Choi,Young  Min; Ahn,Kyu  Joong; An,In-Sook; Cha,Hwa  Jun

doi:10.3892/ijmm.2017.2866

Phytosphingosine-1-phosphate and epidermal growth factor synergistically restore extracellular matrix in human dermal fibroblasts in vitro and in vivo

Authors:
- Seung Bin Kwon
- Sungkwan An
- Min Jung Kim
- Ka Ram Kim
- Young Min Choi
- Kyu Joong Ahn
- In-Sook An
- Hwa Jun Cha
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Affiliations: KISCS Incorporated, Cheongju-si, Chungcheongbuk-do 361-951, Republic of Korea, Korea Institute for Skin and Clinical Sciences, Konkuk University, Seoul 143-701, Republic of Korea, Department of Dermatology, Konkuk University School of Medicine, Seoul 143-701, Republic of Korea
Published online on: January 23, 2017 https://doi.org/10.3892/ijmm.2017.2866
Pages: 741-748

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Abstract

Phytosphingosine-1-phosphate (PhS1P), which is found in plants and fungi, is generated by the phosphorylation of phytosphingosine and is structurally similar to molecules that promote cellular growth and proliferation. The aim of this study was to ascertain whether PhS1P displays synergistic effects together with epidermal growth factor (EGF), which is also critical for activating proliferation, migration and survival pathways. We utilized cultured human dermal fibroblasts (HDFs) and a number of assays, including western blotting, cell migration assays, quantitative (real-time) PCR, and viability assays. We found that PhS1P promoted the activity of EGF in vitro. We then conducted a clinical trial in females over 35 years of age, with visible signs of skin aging. By evaluating skin hydration, dermal density and thickness, length of fine wrinkles, and skin elasticity, we verified the clinical efficacy of a combined treatment of PhS1P and EGF in vivo. On the whole, our data suggest that PhS1P displays a synergistic anti-aging effect together with EGF, both in vitro and in vivo.

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