Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
International Journal of Molecular Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1107-3756 Online ISSN: 1791-244X
Journal Cover
April-2017 Volume 39 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
April-2017 Volume 39 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

Overexpression of TMPRSS4 promotes tumor proliferation and aggressiveness in breast cancer

  • Authors:
    • Xiao-Mei Li
    • Wen-Lou Liu
    • Xu Chen
    • Ya-Wen Wang
    • Duan-Bo Shi
    • Hui Zhang
    • Ran-Ran Ma
    • Hai-Ting Liu
    • Xiang-Yu Guo
    • Feng Hou
    • Ming Li
    • Peng Gao
  • View Affiliations / Copyright

    Affiliations: Department of Pathology, Qilu Hospital of Shandong University, Jinan, Shandong 250012, P.R. China, Department of Oncology, Zhongnan Hospital of Wuhan University, Wuhan, Hubei 430071, P.R. China, Department of Pathology, Affiliated Hospital of Qingdao University, Qingdao, Shandong 266000, P.R. China, Department of Pathology, Dezhou Renmin Hospital, Dezhou, Shandong 253000, P.R. China
    Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 927-935
    |
    Published online on: February 17, 2017
       https://doi.org/10.3892/ijmm.2017.2893
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Transmembrane protease serine 4 (TMPRSS4) is a novel type II transmembrane serine protease that is overexpressed in various types of human cancers and has an important function in cancer progression. However, there is a paucity of data available regarding the biological effects of TMPRSS4 on breast cancer (BC) cells and the underlying mechanisms. In this study, expression of TMPRSS4 in BC tissues was detected by immunohistochemistry. The relationship between TMPRSS4 expression and clinicopathological characteristics as well as prognosis was evaluated. The effects of TMPRSS4 on cell proliferation, migration and invasion were investigated in BC cell lines in vitro. Additionally, RT-qPCR and western blot analysis were used to determine the expressions of epithelial-mesenchymal transition (EMT) biomarkers and TMPRSS4 in BC cell lines. We found that TMPRSS4 was overexpressed in BC tissues and its expression level was closely correlated with tumor size, histological grade, lymph node metastasis, clinical stage as well as poor survival (all P<0.05) and could be recognized as an independent prognostic factor for BC patients. Overexpression of TMPRSS4 promoted the proliferation, migration and invasion of BC cells in vitro. Moreover, TMPRSS4 knockdown significantly enhanced the expression of E-cadherin and claudin-1 and inhibited the expression of vimentin and Slug, indicating suppression of EMT. Our results suggest that TMPRSS4 plays a crucial role in the progression of BC. Moreover, TMPRSS4 overexpression promoted the proliferation, invasion and migration of BC cells by possibly inducing EMT. To conclude, TMPRSS4 may be a potential therapeutic target for cancer treatment.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

View References

1 

Miao H, Hartman M, Bhoo-Pathy N, Lee SC, Taib NA, Tan EY, Chan P, Moons KG, Wong HS, Goh J, et al: Predicting survival of de novo metastatic breast cancer in Asian women: Systematic review and validation study. PLoS One. 9:e937552014. View Article : Google Scholar : PubMed/NCBI

2 

Cummings MC, Chambers R, Simpson PT and Lakhani SR: Molecular classification of breast cancer: Is it time to pack up our microscopes? Pathology. 43:1–8. 2011. View Article : Google Scholar : PubMed/NCBI

3 

Ginsburg GS and Willard HF: Genomic and personalized medicine: Foundations and applications. Transl Res. 154:277–287. 2009. View Article : Google Scholar : PubMed/NCBI

4 

Dechaphunkul A, Phukaoloun M, Kanjanapradit K, Graham K, Ghosh S, Santos C and Mackey JR: Prognostic significance of tissue inhibitor of metalloproteinase-1 in breast cancer. Int J Breast Cancer. 2012:2908542012. View Article : Google Scholar : PubMed/NCBI

5 

Geyer FC, Rodrigues DN, Weigelt B and Reis-Filho JS: Molecular classification of estrogen receptor-positive/luminal breast cancers. Adv Anat Pathol. 19:39–53. 2012. View Article : Google Scholar

6 

Duffy MJ: Proteases as prognostic markers in cancer. Clin Cancer Res. 2:613–618. 1996.PubMed/NCBI

7 

Roy R, Yang J and Moses MA: Matrix metalloproteinases as novel biomarkers and potential therapeutic targets in human cancer. J Clin Oncol. 27:5287–5297. 2009. View Article : Google Scholar : PubMed/NCBI

8 

Flores-Reséndiz D, Castellanos-Juárez E and Benítez-Bribiesca L: Proteases in cancer progression. Gac Med Mex. 145:131–142. 2009.In Spanish.

9 

Netzel-Arnett S, Hooper JD, Szabo R, Madison EL, Quigley JP, Bugge TH and Antalis TM: Membrane anchored serine proteases: A rapidly expanding group of cell surface proteolytic enzymes with potential roles in cancer. Cancer Metastasis Rev. 22:237–258. 2003. View Article : Google Scholar : PubMed/NCBI

10 

Hooper JD, Clements JA, Quigley JP and Antalis TM: Type II transmembrane serine proteases. Insights into an emerging class of cell surface proteolytic enzymes. J Biol Chem. 276:857–860. 2001. View Article : Google Scholar

11 

Choi SY, Bertram S, Glowacka I, Park YW and Pöhlmann S: Type II transmembrane serine proteases in cancer and viral infections. Trends Mol Med. 15:303–312. 2009. View Article : Google Scholar : PubMed/NCBI

12 

de Aberasturi AL and Calvo A: TMPRSS4: An emerging potential therapeutic target in cancer. Br J Cancer. 112:4–8. 2015. View Article : Google Scholar :

13 

Jung H, Lee KP, Park SJ, Park JH, Jang YS, Choi SY, Jung JG, Jo K, Park DY, Yoon JH, et al: TMPRSS4 promotes invasion, migration and metastasis of human tumor cells by facilitating an epithelial-mesenchymal transition. Oncogene. 27:2635–2647. 2008. View Article : Google Scholar

14 

Kim S, Kang HY, Nam EH, Choi MS, Zhao XF, Hong CS, Lee JW, Lee JH and Park YK: TMPRSS4 induces invasion and epithelial-mesenchymal transition through upregulation of integrin alpha5 and its signaling pathways. Carcinogenesis. 31:597–606. 2010. View Article : Google Scholar : PubMed/NCBI

15 

Wang CH, Guo ZY, Chen ZT, Zhi XT, Li DK, Dong ZR, Chen ZQ, Hu SY and Li T: TMPRSS4 facilitates epithelial-mesenchymal transition of hepatocellular carcinoma and is a predictive marker for poor prognosis of patients after curative resection. Sci Rep. 5:123662015. View Article : Google Scholar : PubMed/NCBI

16 

Edge SB, Byrd DR, Compton CC, Fritz AG, Greene FL and Trotti A: : AJCC Cancer Staging Manual. 7th edition. Springer; New York, NY: pp. 237–246. 2010

17 

Lakhani SR, Ellis IO, Schnitt SJ, Tan PH and van de Vijver MJ: WHO classification of tumours of the breast. World Health Organization classification of tumours. 4th edition. IARC Press; Lyon: 2012

18 

Wolff AC, Hammond ME, Hicks DG, Dowsett M, McShane LM, Allison KH, Allred DC, Bartlett JM, Bilous M, Fitzgibbons P, et al American Society of Clinical Oncology; College of American Pathologists: Recommendations for human epidermal growth factor receptor 2 testing in breast cancer: American Society of Clinical Oncology/College of American Pathologists clinical practice guideline update. J Clin Oncol. 31:3997–4013. 2013. View Article : Google Scholar : PubMed/NCBI

19 

Szabo R and Bugge TH: Type II transmembrane serine proteases in development and disease. Int J Biochem Cell Biol. 40:1297–1316. 2008. View Article : Google Scholar : PubMed/NCBI

20 

Antalis TM, Bugge TH and Wu Q: Membrane-anchored serine proteases in health and disease. Prog Mol Biol Transl Sci. 99:1–50. 2011. View Article : Google Scholar : PubMed/NCBI

21 

Wallrapp C, Hähnel S, Müller-Pillasch F, Burghardt B, Iwamura T, Ruthenbürger M, Lerch MM, Adler G and Gress TM: A novel transmembrane serine protease (TMPRSS3) overexpressed in pancreatic cancer. Cancer Res. 60:2602–2606. 2000.PubMed/NCBI

22 

Kebebew E, Peng M, Reiff E, Duh QY, Clark OH and McMillan A: ECM1 and TMPRSS4 are diagnostic markers of malignant thyroid neoplasms and improve the accuracy of fine needle aspiration biopsy. Ann Surg. 242:353–363. 2005.PubMed/NCBI

23 

Nguyen TH, Weber W, Havari E, Connors T, Bagley RG, McLaren R, Nambiar PR, Madden SL, Teicher BA, Roberts B, et al: Expression of TMPRSS4 in non-small cell lung cancer and its modulation by hypoxia. Int J Oncol. 41:829–838. 2012.PubMed/NCBI

24 

Larzabal L, Nguewa PA, Pio R, Blanco D, Sanchez B, Rodríguez MJ, Pajares MJ, Catena R, Montuenga LM and Calvo A: Overexpression of TMPRSS4 in non-small cell lung cancer is associated with poor prognosis in patients with squamous histology. Br J Cancer. 105:1608–1614. 2011. View Article : Google Scholar : PubMed/NCBI

25 

Huang A, Zhou H, Zhao H, Quan Y, Feng B and Zheng M: TMPRSS4 correlates with colorectal cancer pathological stage and regulates cell proliferation and self-renewal ability. Cancer Biol Ther. 15:297–304. 2014. View Article : Google Scholar :

26 

Liang B, Wu M, Bu Y, Zhao A and Xie F: Prognostic value of TMPRSS4 expression in patients with breast cancer. Med Oncol. 30:4972013. View Article : Google Scholar : PubMed/NCBI

27 

Cheng D, Kong H and Li Y: TMPRSS4 as a poor prognostic factor for triple-negative breast cancer. Int J Mol Sci. 14:14659–14668. 2013. View Article : Google Scholar : PubMed/NCBI

28 

Hamamoto J, Soejima K, Naoki K, Yasuda H, Hayashi Y, Yoda S, Nakayama S, Satomi R, Terai H, Ikemura S, et al: Methylation-induced downregulation of TFPI-2 causes TMPRSS4 overexpression and contributes to oncogenesis in a subset of non-small-cell lung carcinoma. Cancer Sci. 106:34–42. 2015. View Article : Google Scholar :

29 

Brockhausen J, Tay SS, Grzelak CA, Bertolino P, Bowen DG, d'Avigdor WM, Teoh N, Pok S, Shackel N, Gamble JR, et al: miR-181a mediates TGF-β-induced hepatocyte EMT and is dysregulated in cirrhosis and hepatocellular cancer. Liver Int. 35:240–253. 2015. View Article : Google Scholar

30 

Xu J, Li X, Yang H, Chang R, Kong C and Yang L: SIN1 promotes invasion and metastasis of hepatocellular carcinoma by facilitating epithelial-mesenchymal transition. Cancer. 119:2247–2257. 2013. View Article : Google Scholar : PubMed/NCBI

31 

Choi SY, Shin HC, Kim SY and Park YW: Role of TMPRSS4 during cancer progression. Drug News Perspect. 21:417–423. 2008.PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Li X, Liu W, Chen X, Wang Y, Shi D, Zhang H, Ma R, Liu H, Guo X, Hou F, Hou F, et al: Overexpression of TMPRSS4 promotes tumor proliferation and aggressiveness in breast cancer. Int J Mol Med 39: 927-935, 2017.
APA
Li, X., Liu, W., Chen, X., Wang, Y., Shi, D., Zhang, H. ... Gao, P. (2017). Overexpression of TMPRSS4 promotes tumor proliferation and aggressiveness in breast cancer. International Journal of Molecular Medicine, 39, 927-935. https://doi.org/10.3892/ijmm.2017.2893
MLA
Li, X., Liu, W., Chen, X., Wang, Y., Shi, D., Zhang, H., Ma, R., Liu, H., Guo, X., Hou, F., Li, M., Gao, P."Overexpression of TMPRSS4 promotes tumor proliferation and aggressiveness in breast cancer". International Journal of Molecular Medicine 39.4 (2017): 927-935.
Chicago
Li, X., Liu, W., Chen, X., Wang, Y., Shi, D., Zhang, H., Ma, R., Liu, H., Guo, X., Hou, F., Li, M., Gao, P."Overexpression of TMPRSS4 promotes tumor proliferation and aggressiveness in breast cancer". International Journal of Molecular Medicine 39, no. 4 (2017): 927-935. https://doi.org/10.3892/ijmm.2017.2893
Copy and paste a formatted citation
x
Spandidos Publications style
Li X, Liu W, Chen X, Wang Y, Shi D, Zhang H, Ma R, Liu H, Guo X, Hou F, Hou F, et al: Overexpression of TMPRSS4 promotes tumor proliferation and aggressiveness in breast cancer. Int J Mol Med 39: 927-935, 2017.
APA
Li, X., Liu, W., Chen, X., Wang, Y., Shi, D., Zhang, H. ... Gao, P. (2017). Overexpression of TMPRSS4 promotes tumor proliferation and aggressiveness in breast cancer. International Journal of Molecular Medicine, 39, 927-935. https://doi.org/10.3892/ijmm.2017.2893
MLA
Li, X., Liu, W., Chen, X., Wang, Y., Shi, D., Zhang, H., Ma, R., Liu, H., Guo, X., Hou, F., Li, M., Gao, P."Overexpression of TMPRSS4 promotes tumor proliferation and aggressiveness in breast cancer". International Journal of Molecular Medicine 39.4 (2017): 927-935.
Chicago
Li, X., Liu, W., Chen, X., Wang, Y., Shi, D., Zhang, H., Ma, R., Liu, H., Guo, X., Hou, F., Li, M., Gao, P."Overexpression of TMPRSS4 promotes tumor proliferation and aggressiveness in breast cancer". International Journal of Molecular Medicine 39, no. 4 (2017): 927-935. https://doi.org/10.3892/ijmm.2017.2893
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team