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Article Open Access

Albumin-based nanoparticles as methylprednisolone carriers for targeted delivery towards the neonatal Fc receptor in glomerular podocytes

  • Authors:
    • Lin Wu
    • Mingyu Chen
    • Huijuan Mao
    • Ningning Wang
    • Bo Zhang
    • Xiufen Zhao
    • Jun Qian
    • Changying Xing
  • View Affiliations / Copyright

    Affiliations: Department of Nephrology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029, P.R. China
    Copyright: © Wu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 851-860
    |
    Published online on: February 21, 2017
       https://doi.org/10.3892/ijmm.2017.2902
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Abstract

Glucocorticoids (GCs) are commonly used in the treatment of nephrotic syndrome. However, high doses and long periods of GC therapy can result in severe side effects. The present study aimed to selectively deliver albumin‑methylprednisolone (MP) nanoparticles towards glomerular podocytes, which highly express the specific neonatal Fc receptor (FcRn) of albumin. Bovine serum albumin (BSA) was labeled with a fluorescent dye and linked with modified MP via an amide bond. The outcome nanoparticle named BSA633‑MP showed a uniform size with a diameter of approximately 10 nm and contained 12 drug molecules on average. The nanoconjugates were found to be stable at pH 7.4 and acid‑sensitive at pH 4.0, with approximately 72% release of the MP drug after 48 h of incubation. The nanoparticle demonstrated a 36‑fold uptake in receptor‑specific cellular delivery in the FcRn‑expressing human podocytes compared to the uptake in the non-FcRn-expressing control cells. Co‑localization further confirmed that uptake of the nanoconjugates involved receptor‑mediated endocytosis followed by lysosome associated transportation. In vitro cellular experiments indicated that the BSA633‑MP ameliorated puromycin aminonucleoside‑induced podocyte apoptosis. Moreover, in vivo fluorescence molecular imaging showed that BSA633-MP was mainly accumulated in the liver and kidney after intravenous dosing for 24 h. Collectively, this study may provide an approach for the effective and safe therapy of nephrotic syndrome.
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View References

1 

Hogan J and Radhakrishnan J: The treatment of idiopathic focal segmental glomerulosclerosis in adults. Adv Chronic Kidney Dis. 21:434–441. 2014. View Article : Google Scholar : PubMed/NCBI

2 

Andolino TP and Reid Adam J: Nephrotic syndrome. Pediatr Rev. 36:117–125. 2015. View Article : Google Scholar : PubMed/NCBI

3 

Tran TH, J Hughes G, Greenfeld C and Pham JT: Overview of current and alternative therapies for idiopathic membranous nephropathy. Pharmacotherapy. 35:396–411. 2015. View Article : Google Scholar : PubMed/NCBI

4 

Rezende GM, Viana VS, Malheiros DM, Borba EF, Silva NA, Silva C, Leon EP, Noronha IL and Bonfa E: Podocyte injury in pure membranous and proliferative lupus nephritis: Distinct underlying mechanisms of proteinuria? Lupus. 23:255–262. 2014. View Article : Google Scholar

5 

Akchurin O and Reidy KJ: Genetic causes of proteinuria and nephrotic syndrome: Impact on podocyte pathobiology. Pediatr Nephrol. 30:221–233. 2015. View Article : Google Scholar

6 

Yan K, Kudo A, Hirano H, Watanabe T, Tasaka T, Kataoka S, Nakajima N, Nishibori Y, Shibata T, Kohsaka T, et al: Subcellular localization of glucocorticoid receptor protein in the human kidney glomerulus. Kidney Int. 56:65–73. 1999. View Article : Google Scholar : PubMed/NCBI

7 

Xing CY, Saleem MA, Coward RJ, Ni L, Witherden IR and Mathieson PW: Direct effects of dexamethasone on human podocytes. Kidney Int. 70:1038–1045. 2006. View Article : Google Scholar : PubMed/NCBI

8 

Ransom RF, Lam NG, Hallett MA, Atkinson SJ and Smoyer WE: Glucocorticoids protect and enhance recovery of cultured murine podocytes via actin filament stabilization. Kidney Int. 68:2473–2483. 2005. View Article : Google Scholar : PubMed/NCBI

9 

Wada T, Pippin JW, Marshall CB, Griffin SV and Shankland SJ: Dexamethasone prevents podocyte apoptosis induced by puromycin aminonucleoside: Role of P53 and Bcl-2-related family proteins. J Am Soc Nephrol. 16:2615–2625. 2005. View Article : Google Scholar : PubMed/NCBI

10 

Agrawal S, Guess AJ, Benndorf R and Smoyer WE: Comparison of direct action of thiazolidinediones and glucocorticoids on renal podocytes: Protection from injury and molecular effects. Mol Pharmacol. 80:389–399. 2011. View Article : Google Scholar : PubMed/NCBI

11 

Liu H, Gao X, Xu H, Feng C, Kuang X, Li Z and Zha X: α-Actinin-4 is involved in the process by which dexamethasone protects actin cytoskeleton stabilization from adriamycin-induced podocyte injury. Nephrology (Carlton). 17:669–675. 2012. View Article : Google Scholar

12 

Yu SY and Qi R: Role of bad in podocyte apoptosis induced by puromycin aminonucleoside. Transplant Proc. 45:569–573. 2013. View Article : Google Scholar : PubMed/NCBI

13 

Ohashi T, Uchida K, Uchida S, Sasaki S and Nitta K: Dexamethasone increases the phosphorylation of nephrin in cultured podocytes. Clin Exp Nephrol. 15:688–693. 2011. View Article : Google Scholar : PubMed/NCBI

14 

Mao Y, Triantafillou G, Hertlein E, Towns W, Stefanovski M, Mo X, Jarjoura D, Phelps M, Marcucci G, Lee LJ, et al: Milatuzumab-conjugated liposomes as targeted dexamethasone carriers for therapeutic delivery in CD74 B-cell malignancies. Clinical cancer research: Clin Cancer Res. 19:347–356. 2013. View Article : Google Scholar

15 

Graversen JH, Svendsen P, Dagnæs-Hansen F, Dal J, Anton G, Etzerodt A, Petersen MD, Christensen PA, Møller HJ and Moestrup SK: Targeting the hemoglobin scavenger receptor CD163 in macrophages highly increases the anti-inflammatory potency of dexamethasone. Mol Ther. 20:1550–1558. 2012. View Article : Google Scholar : PubMed/NCBI

16 

Asgeirsdóttir SA, Kamps JA, Bakker HI, Zwiers PJ, Heeringa P, van der Weide K, van Goor H, Petersen AH, Morselt H, Moorlag HE, et al: Site-specific inhibition of glomerulonephritis progression by targeted delivery of dexamethasone to glomerular endothelium. Mol Pharmacol. 72:121–131. 2007. View Article : Google Scholar : PubMed/NCBI

17 

Asgeirsdóttir SA, Zwiers PJ, Morselt HW, Moorlag HE, Bakker HI, Heeringa P, Kok JW, Kallenberg CG, Molema G and Kamps JA: Inhibition of proinflammatory genes in anti-GBM glomerulonephritis by targeted dexamethasone-loaded AbEsel liposomes. Am J Physiol Renal Physiol. 294:F554–F561. 2008. View Article : Google Scholar

18 

Wang Y, Tian Z, Thirumalai D and Zhang X: Neonatal Fc receptor (FcRn): A novel target for therapeutic antibodies and antibody engineering. J Drug Target. 22:269–278. 2014. View Article : Google Scholar : PubMed/NCBI

19 

Eyre J, Ioannou K, Grubb BD, Saleem MA, Mathieson PW, Brunskill NJ, Christensen EI and Topham PS: Statin-sensitive endocytosis of albumin by glomerular podocytes. Am J Physiol Renal Physiol. 292:F674–F681. 2007. View Article : Google Scholar

20 

Haymann JP, Levraud JP, Bouet S, Kappes V, Hagège J, Nguyen G, Xu Y, Rondeau E and Sraer JD: Characterization and localization of the neonatal Fc receptor in adult human kidney. J Am Soc Nephrol. 11:632–639. 2000.PubMed/NCBI

21 

Wu L, Wu J, Zhou Y, Tang X, Du Y and Hu Y: Enhanced antitumor efficacy of cisplatin by tirapazamine-transferrin conjugate. Int J Pharm. 431:190–196. 2012. View Article : Google Scholar : PubMed/NCBI

22 

Kufleitner J, Wagner S, Worek F, von Briesen H and Kreuter J: Adsorption of obidoxime onto human serum albumin nanoparticles: Drug loading, particle size and drug release. J Microencapsul. 27:506–513. 2010. View Article : Google Scholar : PubMed/NCBI

23 

Subia B and Kundu SC: Drug loading and release on tumor cells using silk fibroin-albumin nanoparticles as carriers. Nanotechnology. 24:0351032013. View Article : Google Scholar

24 

Della Porta G, Adami R, Del Gaudio P, Prota L, Aquino R and Reverchon E: Albumin-gentamicin microspheres produced by supercritical assisted atomization: Optimization of size, drug loading and release. J Pharm Sci. 99:4720–4729. 2010. View Article : Google Scholar : PubMed/NCBI

25 

Kratz F: A clinical update of using albumin as a drug vehicle - a commentary. J Control Release. 190:331–336. 2014. View Article : Google Scholar : PubMed/NCBI

26 

Rodrigues PC, Roth T, Fiebig HH, Unger C, Mülhaupt R and Kratz F: Correlation of the acid-sensitivity of polyethylene glycol daunorubicin conjugates with their in vitro antiproliferative activity. Bioorg Med Chem. 14:4110–4117. 2006. View Article : Google Scholar : PubMed/NCBI

27 

Li Y, Du Y, Liu X, Zhang Q, Jing L, Liang X, Chi C, Dai Z and Tian J: Monitoring tumor targeting and treatment effects of IRDye 800CW and GX1-conjugated polylactic acid nanoparticles encapsulating endostar on glioma by optical molecular imaging. Mol Imaging. 14:356–365. 2015.PubMed/NCBI

28 

Francisco AF, Lewis MD, Jayawardhana S, Taylor MC, Chatelain E and Kelly JM: Limited ability of posaconazole To cure both acute and chronic Trypanosoma cruzi infections revealed by highly sensitive in vivo imaging. Antimicrob Agents Chemother. 59:4653–4661. 2015. View Article : Google Scholar : PubMed/NCBI

29 

Zhang C, Liu T, Su Y, Luo S, Zhu Y, Tan X, Fan S, Zhang L, Zhou Y, Cheng T, et al: A near-infrared fluorescent heptamethine indocyanine dye with preferential tumor accumulation for in vivo imaging. Biomaterials. 31:6612–6617. 2010. View Article : Google Scholar : PubMed/NCBI

30 

Neurath MF: Molecular Endoscopy and in vivo Imaging in inflammatory bowel diseases. Dig Dis. 33(Suppl 1): 32–36. 2015. View Article : Google Scholar : PubMed/NCBI

31 

Piwnica-Worms D: From the guest editor: Illuminating cancer in vivo with molecular imaging. Cancer J. 21:150–151. 2015. View Article : Google Scholar : PubMed/NCBI

32 

Chan MM, Gray BD, Pak KY and Fong D: Non-invasive in vivo imaging of arthritis in a collagen-induced murine model with phosphatidylserine-binding near-infrared (NIR) dye. Arthritis Res Ther. 17:502015. View Article : Google Scholar : PubMed/NCBI

33 

Yuan A, Wu J, Tang X, Zhao L, Xu F and Hu Y: Application of near-infrared dyes for tumor imaging, photothermal, and photo-dynamic therapies. J Pharm Sci. 102:6–28. 2013. View Article : Google Scholar

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Copy and paste a formatted citation
Spandidos Publications style
Wu L, Chen M, Mao H, Wang N, Zhang B, Zhao X, Qian J and Xing C: Albumin-based nanoparticles as methylprednisolone carriers for targeted delivery towards the neonatal Fc receptor in glomerular podocytes. Int J Mol Med 39: 851-860, 2017.
APA
Wu, L., Chen, M., Mao, H., Wang, N., Zhang, B., Zhao, X. ... Xing, C. (2017). Albumin-based nanoparticles as methylprednisolone carriers for targeted delivery towards the neonatal Fc receptor in glomerular podocytes. International Journal of Molecular Medicine, 39, 851-860. https://doi.org/10.3892/ijmm.2017.2902
MLA
Wu, L., Chen, M., Mao, H., Wang, N., Zhang, B., Zhao, X., Qian, J., Xing, C."Albumin-based nanoparticles as methylprednisolone carriers for targeted delivery towards the neonatal Fc receptor in glomerular podocytes". International Journal of Molecular Medicine 39.4 (2017): 851-860.
Chicago
Wu, L., Chen, M., Mao, H., Wang, N., Zhang, B., Zhao, X., Qian, J., Xing, C."Albumin-based nanoparticles as methylprednisolone carriers for targeted delivery towards the neonatal Fc receptor in glomerular podocytes". International Journal of Molecular Medicine 39, no. 4 (2017): 851-860. https://doi.org/10.3892/ijmm.2017.2902
Copy and paste a formatted citation
x
Spandidos Publications style
Wu L, Chen M, Mao H, Wang N, Zhang B, Zhao X, Qian J and Xing C: Albumin-based nanoparticles as methylprednisolone carriers for targeted delivery towards the neonatal Fc receptor in glomerular podocytes. Int J Mol Med 39: 851-860, 2017.
APA
Wu, L., Chen, M., Mao, H., Wang, N., Zhang, B., Zhao, X. ... Xing, C. (2017). Albumin-based nanoparticles as methylprednisolone carriers for targeted delivery towards the neonatal Fc receptor in glomerular podocytes. International Journal of Molecular Medicine, 39, 851-860. https://doi.org/10.3892/ijmm.2017.2902
MLA
Wu, L., Chen, M., Mao, H., Wang, N., Zhang, B., Zhao, X., Qian, J., Xing, C."Albumin-based nanoparticles as methylprednisolone carriers for targeted delivery towards the neonatal Fc receptor in glomerular podocytes". International Journal of Molecular Medicine 39.4 (2017): 851-860.
Chicago
Wu, L., Chen, M., Mao, H., Wang, N., Zhang, B., Zhao, X., Qian, J., Xing, C."Albumin-based nanoparticles as methylprednisolone carriers for targeted delivery towards the neonatal Fc receptor in glomerular podocytes". International Journal of Molecular Medicine 39, no. 4 (2017): 851-860. https://doi.org/10.3892/ijmm.2017.2902
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