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MicroRNA-182 prevents vascular smooth muscle cell dedifferentiation via FGF9/PDGFRβ signaling

  • Authors:
    • Nana Dong
    • Wei Wang
    • Jinwei Tian
    • Zulong Xie
    • Bo Lv
    • Jiannan Dai
    • Rui Jiang
    • Dan Huang
    • Shaohong Fang
    • Jiangtian Tian
    • Hulun Li
    • Bo Yu
  • View Affiliations / Copyright

    Affiliations: Department of Cardiology, The Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150086, P.R. China, Key Laboratory of Myocardial Ischemia, Ministry of Education, The Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150086, P.R. China
    Copyright: © Dong et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 791-798
    |
    Published online on: February 22, 2017
       https://doi.org/10.3892/ijmm.2017.2905
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Abstract

The abnormal phenotypic transformation of vascular smooth muscle cells (SMCs) causes various proliferative vascular diseases. MicroRNAs (miRNAs or miRs) have been established to play important roles in SMC biology and phenotypic modulation. This study revealed that the expression of miR‑182 was markedly altered during rat vascular SMC phenotypic transformation in vitro. We aimed to investigate the role of miR‑182 in the vascular SMC phenotypic switch and to determine the potential molecular mechanisms involved. The expression of miR‑182 gene was significantly downregulated in cultured SMCs during dedifferentiation from a contractile to a synthetic phenotype. Conversely, the upregulation of miR‑182 increased the expression of SMC-specific contractile genes, such as α-smooth muscle actin, smooth muscle 22α and calponin. Additionally, miR‑182 overexpression potently inhibited SMC proliferation and migration under both basal conditions and under platelet-derived growth factor-BB stimulation. Furthermore, we identified fibroblast growth factor 9 (FGF9) as the target gene of miR‑182 for the phenotypic modulation of SMCs mediated through platelet-derived growth factor receptor β (PDGFRβ) signaling. These data suggest that miR‑182 may be a novel SMC phenotypic marker and a modulator that may be used to prevent SMC dedifferentiation via FGF9/PDGFRβ signaling.
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Copy and paste a formatted citation
Spandidos Publications style
Dong N, Wang W, Tian J, Xie Z, Lv B, Dai J, Jiang R, Huang D, Fang S, Tian J, Tian J, et al: MicroRNA-182 prevents vascular smooth muscle cell dedifferentiation via FGF9/PDGFRβ signaling. Int J Mol Med 39: 791-798, 2017.
APA
Dong, N., Wang, W., Tian, J., Xie, Z., Lv, B., Dai, J. ... Yu, B. (2017). MicroRNA-182 prevents vascular smooth muscle cell dedifferentiation via FGF9/PDGFRβ signaling. International Journal of Molecular Medicine, 39, 791-798. https://doi.org/10.3892/ijmm.2017.2905
MLA
Dong, N., Wang, W., Tian, J., Xie, Z., Lv, B., Dai, J., Jiang, R., Huang, D., Fang, S., Tian, J., Li, H., Yu, B."MicroRNA-182 prevents vascular smooth muscle cell dedifferentiation via FGF9/PDGFRβ signaling". International Journal of Molecular Medicine 39.4 (2017): 791-798.
Chicago
Dong, N., Wang, W., Tian, J., Xie, Z., Lv, B., Dai, J., Jiang, R., Huang, D., Fang, S., Tian, J., Li, H., Yu, B."MicroRNA-182 prevents vascular smooth muscle cell dedifferentiation via FGF9/PDGFRβ signaling". International Journal of Molecular Medicine 39, no. 4 (2017): 791-798. https://doi.org/10.3892/ijmm.2017.2905
Copy and paste a formatted citation
x
Spandidos Publications style
Dong N, Wang W, Tian J, Xie Z, Lv B, Dai J, Jiang R, Huang D, Fang S, Tian J, Tian J, et al: MicroRNA-182 prevents vascular smooth muscle cell dedifferentiation via FGF9/PDGFRβ signaling. Int J Mol Med 39: 791-798, 2017.
APA
Dong, N., Wang, W., Tian, J., Xie, Z., Lv, B., Dai, J. ... Yu, B. (2017). MicroRNA-182 prevents vascular smooth muscle cell dedifferentiation via FGF9/PDGFRβ signaling. International Journal of Molecular Medicine, 39, 791-798. https://doi.org/10.3892/ijmm.2017.2905
MLA
Dong, N., Wang, W., Tian, J., Xie, Z., Lv, B., Dai, J., Jiang, R., Huang, D., Fang, S., Tian, J., Li, H., Yu, B."MicroRNA-182 prevents vascular smooth muscle cell dedifferentiation via FGF9/PDGFRβ signaling". International Journal of Molecular Medicine 39.4 (2017): 791-798.
Chicago
Dong, N., Wang, W., Tian, J., Xie, Z., Lv, B., Dai, J., Jiang, R., Huang, D., Fang, S., Tian, J., Li, H., Yu, B."MicroRNA-182 prevents vascular smooth muscle cell dedifferentiation via FGF9/PDGFRβ signaling". International Journal of Molecular Medicine 39, no. 4 (2017): 791-798. https://doi.org/10.3892/ijmm.2017.2905
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