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International Journal of Molecular Medicine
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April-2017 Volume 39 Issue 4

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Article

Regulation of Runx2 by microRNA-9 and microRNA-10 modulates the osteogenic differentiation of mesenchymal stem cells

  • Authors:
    • Hong Luo
    • Hualin Gao
    • Fang Liu
    • Bing Qiu
  • View Affiliations / Copyright

    Affiliations: Department of Orthopaedic Surgery, Guizhou Orthopaedics Hospital, Guiyang, Guizhou 550001, P.R. China, Department of Anesthesiology, Guizhou Orthopaedics Hospital, Guiyang, Guizhou 550001, P.R. China
  • Pages: 1046-1052
    |
    Published online on: March 10, 2017
       https://doi.org/10.3892/ijmm.2017.2918
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Abstract

Currently, the use of stem cell transplantation for the treatment of osteoporosis is a popular research topic. In general, promotion of the osteogenic differentiation of stem cells is the goal of researchers. MicroRNAs (miRNAs of miRs) are critical regulatory factors of osteogenic induction, and miR‑9 and miR‑10 have been substantiated to be involved in the osteogenic process, yet the underlying mechanisms remain unclear. The present study investigated the role of miR‑9 and miR‑10 in the osteogenic differentiation of bone marrow-derived mesenchymal stem cells (BMSCs) and aimed to elucidate the mechanism of osteogenic differentiation. Using MTT assay and RT-PCR, we found that miR‑9 expression was upregulated and miR-10 expression was downregulated during osteogenic differentiation. Moreover, as shown by the results of mineralization analysis and western blot analysis, the overexpression of miR‑9 enhanced the osteogenic abilities and overexpression of miR‑9 inhibited these abilities. Furthermore, miR‑9 and miR‑10 also affected the expression of Runt‑related transcription factor 2 (Runx2) and extracellular signal-regulated kinase (ERK) pathway by western blot analysis. Based on these results, we conclude that miR‑9 and miR‑10 play key roles in regulating the differentiation of BMSCs.
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1 

Curtis EM, Moon RJ, Dennison EM, Harvey NC and Cooper C: Recent advances in the pathogenesis and treatment of osteoporosis. Clin Med (Lond). 15(Suppl 6): s92–s96. 2015. View Article : Google Scholar

2 

Piscitelli P, Iolascon G, Gimigliano F, Gimigliano A, Marinelli A, Di Nuzzo R, Colì G, Di Paola L, Gianicolo E, Chitano G, et al: Osteoporosis and cardiovascular diseases' cosegregation: Epidemiological features. Clin Cases Miner Bone Metab. 5:14–18. 2008.PubMed/NCBI

3 

Wang Y, Tao Y, Hyman ME, Li J and Chen Y: Osteoporosis in china. Osteoporos Int. 20:1651–1662. 2009. View Article : Google Scholar : PubMed/NCBI

4 

Lin X, Xiong D, Peng YQ, Sheng ZF, Wu XY, Wu XP, Wu F, Yuan LQ and Liao EY: Epidemiology and management of osteoporosis in the People's Republic of China: Current perspectives. Clin Interv Aging. 10:1017–1033. 2015.PubMed/NCBI

5 

Geusens P: New insights into treatment of osteoporosis in postmenopausal women. RMD Open. 1(Suppl 1): e0000512015. View Article : Google Scholar : PubMed/NCBI

6 

Xie C, Jin J, Lv X, Tao J, Wang R and Miao D: Anti-aging effect of transplanted amniotic membrane mesenchymal stem cells in a premature aging model of Bmi-1 deficiency. Sci Rep. 5:139752015. View Article : Google Scholar : PubMed/NCBI

7 

Ma L, Aijima R, Hoshino Y, Yamaza H, Tomoda E, Tanaka Y, Sonoda S, Song G, Zhao W, Nonaka K, et al: Transplantation of mesenchymal stem cells ameliorates secondary osteoporosis through interleukin-17-impaired functions of recipient bone marrow mesenchymal stem cells in MRL/lpr mice. Stem Cell Res Ther. 6:1042015. View Article : Google Scholar : PubMed/NCBI

8 

Pittenger MF, Mackay AM, Beck SC, Jaiswal RK, Douglas R, Mosca JD, Moorman MA, Simonetti DW, Craig S and Marshak DR: Multilineage potential of adult human mesenchymal stem cells. Science. 284:143–147. 1999. View Article : Google Scholar : PubMed/NCBI

9 

Bartel DP: MicroRNAs: Genomics, biogenesis, mechanism, and function. Cell. 116:281–297. 2004. View Article : Google Scholar : PubMed/NCBI

10 

Fang S, Deng Y, Gu P and Fan X: MicroRNAs regulate bone development and regeneration. Int J Mol Sci. 16:8227–8253. 2015. View Article : Google Scholar : PubMed/NCBI

11 

Zhang GY, Wang J, Jia YJ, Han R, Li P and Zhu DN: MicroRNA-9 promotes the neuronal differentiation of rat bone marrow mesenchymal stem cells by activating autophagy. Neural Regen Res. 10:314–320. 2015. View Article : Google Scholar : PubMed/NCBI

12 

Varendi K, Kumar A, Härma MA and Andressoo JO: miR-1, miR-10b, miR-155, and miR-191 are novel regulators of BDNF. Cell Mol Life Sci. 71:4443–4456. 2014. View Article : Google Scholar : PubMed/NCBI

13 

Shakibaei M, Shayan P, Busch F, Aldinger C, Buhrmann C, Lueders C and Mobasheri A: Resveratrol mediated modulation of Sirt-1/Runx2 promotes osteogenic differentiation of mesenchymal stem cells: Potential role of Runx2 deacetylation. PLoS One. 7:e357122012. View Article : Google Scholar : PubMed/NCBI

14 

Wu Y, Zhou J, Li Y, Zhou Y, Cui Y, Yang G and Hong Y: Rap1A regulates osteoblastic differentiation via the ERK and p38 mediated signaling. PLoS One. 10:e01437772015. View Article : Google Scholar : PubMed/NCBI

15 

Li Y and Sarkar FH: MicroRNA targeted therapeutic approach for pancreatic cancer. Int J Biol Sci. 12:326–337. 2016. View Article : Google Scholar : PubMed/NCBI

16 

Kuninty PR, Schnittert J, Storm G and Prakash J: MicroRNA targeting to modulate tumor microenvironment. Front Oncol. 6:32016. View Article : Google Scholar : PubMed/NCBI

17 

Taylor MA and Schiemann WP: Therapeutic opportunities for targeting microRNAs in cancer. Mol Cell Ther. 2:1–13. 2014. View Article : Google Scholar

18 

Ott CE, Grünhagen J, Jäger M, Horbelt D, Schwill S, Kallenbach K, Guo G, Manke T, Knaus P, Mundlos S, et al: MicroRNAs differentially expressed in postnatal aortic development downregulate elastin via 3′ UTR and coding-sequence binding sites. PLoS One. 6:e162502011. View Article : Google Scholar

19 

Yan J, Guo D, Yang S, Sun H, Wu B and Zhou D: Inhibition of miR-222-3p activity promoted osteogenic differentiation of hBMSCs by regulating Smad5-RUNX2 signal axis. Biochem Biophys Res Commun. 470:498–503. 2016. View Article : Google Scholar : PubMed/NCBI

20 

Weilner S, Skalicky S, Salzer B, Keider V, Wagner M, Hildner F, Gabriel C, Dovjak P, Pietschmann P, Grillari-Voglauer R, et al: Differentially circulating miRNAs after recent osteoporotic fractures can influence osteogenic differentiation. Bone. 79:43–51. 2015. View Article : Google Scholar : PubMed/NCBI

21 

Zhang JF, Fu WM, He ML, Wang H, Wang WM, Yu SC, Bian XW, Zhou J, Lin MC, Lu G, et al: MiR-637 maintains the balance between adipocytes and osteoblasts by directly targeting Osterix. Mol Biol Cell. 22:3955–3961. 2011. View Article : Google Scholar : PubMed/NCBI

22 

Trompeter HI, Dreesen J, Hermann E, Iwaniuk KM, Hafner M, Renwick N, Tuschl T and Wernet P: MicroRNAs miR-26a, miR-26b, and miR-29b accelerate osteogenic differentiation of unrestricted somatic stem cells from human cord blood. BMC Genomics. 14:1112013. View Article : Google Scholar : PubMed/NCBI

23 

Sun J, Fang K, Shen H and Qian Y: MicroRNA-9 is a ponderable index for the prognosis of human hepatocellular carcinoma. Int J Clin Exp Med. 8:17748–17756. 2015.

24 

Drakaki A, Hatziapostolou M, Polytarchou C, Vorvis C, Poultsides GA, Souglakos J, Georgoulias V and Iliopoulos D: Functional microRNA high throughput screening reveals miR-9 as a central regulator of liver oncogenesis by affecting the PPARA-CDH1 pathway. BMC Cancer. 15:5422015. View Article : Google Scholar : PubMed/NCBI

25 

Lund AH: miR-10 in development and cancer. Cell Death Differ. 17:209–214. 2010. View Article : Google Scholar

26 

Woltering JM and Durston AJ: MiR-10 represses HoxB1a and HoxB3a in zebrafish. PLoS One. 3:e13962008. View Article : Google Scholar : PubMed/NCBI

27 

Xu J, Li Z, Hou Y and Fang W: Potential mechanisms underlying the Runx2 induced osteogenesis of bone marrow mesenchymal stem cells. Am J Transl Res. 7:2527–2535. 2015.

28 

Choung HW, Lee DS, Lee HK, Shon WJ and Park JC: Preameloblast-derived factors mediate osteoblast differentiation of human bone marrow mesenchymal stem cells by Runx2-osterix-BSP signaling. Tissue Eng Part A. 22:93–102. 2016. View Article : Google Scholar

29 

Salasznyk RM, Klees RF, Hughlock MK and Plopper GE: ERK signaling pathways regulate the osteogenic differentiation of human mesenchymal stem cells on collagen I and vitronectin. Cell Commun Adhes. 11:137–153. 2004. View Article : Google Scholar

30 

Saito T, Nishida K, Furumatsu T, Yoshida A, Ozawa M and Ozaki T: Histone deacetylase inhibitors suppress mechanical stress-induced expression of RUNX-2 and ADAMTS-5 through the inhibition of the MAPK signaling pathway in cultured human chondrocytes. Osteoarthritis Cartilage. 21:165–174. 2013. View Article : Google Scholar

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Copy and paste a formatted citation
Spandidos Publications style
Luo H, Gao H, Liu F and Qiu B: Regulation of Runx2 by microRNA-9 and microRNA-10 modulates the osteogenic differentiation of mesenchymal stem cells. Int J Mol Med 39: 1046-1052, 2017.
APA
Luo, H., Gao, H., Liu, F., & Qiu, B. (2017). Regulation of Runx2 by microRNA-9 and microRNA-10 modulates the osteogenic differentiation of mesenchymal stem cells. International Journal of Molecular Medicine, 39, 1046-1052. https://doi.org/10.3892/ijmm.2017.2918
MLA
Luo, H., Gao, H., Liu, F., Qiu, B."Regulation of Runx2 by microRNA-9 and microRNA-10 modulates the osteogenic differentiation of mesenchymal stem cells". International Journal of Molecular Medicine 39.4 (2017): 1046-1052.
Chicago
Luo, H., Gao, H., Liu, F., Qiu, B."Regulation of Runx2 by microRNA-9 and microRNA-10 modulates the osteogenic differentiation of mesenchymal stem cells". International Journal of Molecular Medicine 39, no. 4 (2017): 1046-1052. https://doi.org/10.3892/ijmm.2017.2918
Copy and paste a formatted citation
x
Spandidos Publications style
Luo H, Gao H, Liu F and Qiu B: Regulation of Runx2 by microRNA-9 and microRNA-10 modulates the osteogenic differentiation of mesenchymal stem cells. Int J Mol Med 39: 1046-1052, 2017.
APA
Luo, H., Gao, H., Liu, F., & Qiu, B. (2017). Regulation of Runx2 by microRNA-9 and microRNA-10 modulates the osteogenic differentiation of mesenchymal stem cells. International Journal of Molecular Medicine, 39, 1046-1052. https://doi.org/10.3892/ijmm.2017.2918
MLA
Luo, H., Gao, H., Liu, F., Qiu, B."Regulation of Runx2 by microRNA-9 and microRNA-10 modulates the osteogenic differentiation of mesenchymal stem cells". International Journal of Molecular Medicine 39.4 (2017): 1046-1052.
Chicago
Luo, H., Gao, H., Liu, F., Qiu, B."Regulation of Runx2 by microRNA-9 and microRNA-10 modulates the osteogenic differentiation of mesenchymal stem cells". International Journal of Molecular Medicine 39, no. 4 (2017): 1046-1052. https://doi.org/10.3892/ijmm.2017.2918
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