Regulation of Runx2 by microRNA-9 and microRNA-10 modulates the osteogenic differentiation of mesenchymal stem cells

Luo,Hong; Gao,Hualin; Liu,Fang; Qiu,Bing

doi:10.3892/ijmm.2017.2918

Regulation of Runx2 by microRNA-9 and microRNA-10 modulates the osteogenic differentiation of mesenchymal stem cells

Authors:
- Hong Luo
- Hualin Gao
- Fang Liu
- Bing Qiu
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Affiliations: Department of Orthopaedic Surgery, Guizhou Orthopaedics Hospital, Guiyang, Guizhou 550001, P.R. China, Department of Anesthesiology, Guizhou Orthopaedics Hospital, Guiyang, Guizhou 550001, P.R. China
Published online on: March 10, 2017 https://doi.org/10.3892/ijmm.2017.2918
Pages: 1046-1052

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Abstract

Currently, the use of stem cell transplantation for the treatment of osteoporosis is a popular research topic. In general, promotion of the osteogenic differentiation of stem cells is the goal of researchers. MicroRNAs (miRNAs of miRs) are critical regulatory factors of osteogenic induction, and miR‑9 and miR‑10 have been substantiated to be involved in the osteogenic process, yet the underlying mechanisms remain unclear. The present study investigated the role of miR‑9 and miR‑10 in the osteogenic differentiation of bone marrow-derived mesenchymal stem cells (BMSCs) and aimed to elucidate the mechanism of osteogenic differentiation. Using MTT assay and RT-PCR, we found that miR‑9 expression was upregulated and miR-10 expression was downregulated during osteogenic differentiation. Moreover, as shown by the results of mineralization analysis and western blot analysis, the overexpression of miR‑9 enhanced the osteogenic abilities and overexpression of miR‑9 inhibited these abilities. Furthermore, miR‑9 and miR‑10 also affected the expression of Runt‑related transcription factor 2 (Runx2) and extracellular signal-regulated kinase (ERK) pathway by western blot analysis. Based on these results, we conclude that miR‑9 and miR‑10 play key roles in regulating the differentiation of BMSCs.

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