Open Access

Designer D-form self-assembling peptide scaffolds promote the proliferation and migration of rat bone marrow-derived mesenchymal stem cells

  • Authors:
    • Shuo Chen
    • Ao Zhou
    • Bin He
    • Weikang Zhao
    • Xiaojun Chen
    • Dianming Jiang
  • View Affiliations

  • Published online on: July 5, 2017     https://doi.org/10.3892/ijmm.2017.3056
  • Pages: 679-688
  • Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Self-assembling peptide (SAP) nanofiber hydrogel scaffolds have become increasingly important in tissue engineering due to their outstanding bioactivity and biodegradability. However, there is an initial concern on their long-term clinical use, since SAPs made of L-form amino acid sequences are sensitive to enzymatic degradation. In this study, we present a designer SAP, D-RADA16, made of all D-amino acid. We investigated the nanofiber morphology of D-RADA16, its potential for the culture of bone marrow-derived mesenchymal stem cells (BMSCs), and the proteolytic resistance of the biomaterial. The results revealed that D-RADA16 exhibited stable β-sheets and formed interwoven nanofiber scaffolds in water. D-RADA16 and L-RADA16 hydrogel scaffolds were both found to promote the proliferation and migration of rat BMSCs in the 3D cell culture microenvironment. Furthermore, the D-RADA16 scaffolds exhibited a higher proteolytic resistance against proteinase K than the L-RADA16 scaffolds. These observations indicate that D-RADA16 hydrogel scaffolds have excellent bioactivity, biocompatibility and biostability, and thus may serve as promising candidates for long-term application in vivo.
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September-2017
Volume 40 Issue 3

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Chen S, Zhou A, He B, Zhao W, Chen X and Jiang D: Designer D-form self-assembling peptide scaffolds promote the proliferation and migration of rat bone marrow-derived mesenchymal stem cells. Int J Mol Med 40: 679-688, 2017
APA
Chen, S., Zhou, A., He, B., Zhao, W., Chen, X., & Jiang, D. (2017). Designer D-form self-assembling peptide scaffolds promote the proliferation and migration of rat bone marrow-derived mesenchymal stem cells. International Journal of Molecular Medicine, 40, 679-688. https://doi.org/10.3892/ijmm.2017.3056
MLA
Chen, S., Zhou, A., He, B., Zhao, W., Chen, X., Jiang, D."Designer D-form self-assembling peptide scaffolds promote the proliferation and migration of rat bone marrow-derived mesenchymal stem cells". International Journal of Molecular Medicine 40.3 (2017): 679-688.
Chicago
Chen, S., Zhou, A., He, B., Zhao, W., Chen, X., Jiang, D."Designer D-form self-assembling peptide scaffolds promote the proliferation and migration of rat bone marrow-derived mesenchymal stem cells". International Journal of Molecular Medicine 40, no. 3 (2017): 679-688. https://doi.org/10.3892/ijmm.2017.3056