Soyasaponin Ag inhibits α‑MSH‑induced melanogenesis in B16F10 melanoma cells via the downregulation of TRP‑2

Yang,Seung Hwan; Tsatsakis,Aristides M.; Tzanakakis,George; Kim,Hong‑Suk; Le,Bao; Sifaki,Maria; Spandidos,Demetrios A.; Tsukamoto,Chigen; Golokhvast,Kirill S.; Izotov,Boris N.; Chung,Gyuhwa

doi:10.3892/ijmm.2017.3061

Soyasaponin Ag inhibits α‑MSH‑induced melanogenesis in B16F10 melanoma cells via the downregulation of TRP‑2

Authors:
- Seung Hwan Yang
- Aristides M. Tsatsakis
- George Tzanakakis
- Hong‑Suk Kim
- Bao Le
- Maria Sifaki
- Demetrios A. Spandidos
- Chigen Tsukamoto
- Kirill S. Golokhvast
- Boris N. Izotov
- Gyuhwa Chung
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Affiliations: Department of Biotechnology, Chonnam National University, Yeosu, Chonnam 59626, Republic of Korea, Laboratory of Toxicology, Medical School, University of Crete, Heraklion 71003, Greece, Laboratory of Anatomy‑Histology‑Embryology, School of Medicine, University of Crete, Heraklion 71003, Greece, Durae Corporation, Research and Development Center, Gunpo, Gyeonggi 435‑832, Republic of Korea, Laboratory of Virology, Medical School, University of Crete, Heraklion 71003, Greece, Faculty of Agriculture, Iwate University, Morioka, Iwate 020‑8550, Japan, Educational Scientific Center of Nanotechnology, Engineering School, Far Eastern Federal University, Vladivostok 690950, Russia, Department of Analytical Toxicology, Pharmaceutical Chemistry and Pharmacognosy, Sechenov University, Moscow 119991, Russia
Published online on: July 10, 2017 https://doi.org/10.3892/ijmm.2017.3061
Pages: 631-636
Copyright: © Yang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Saponins, which are glycosylated, represent a diverse group of biologically functional products in plants. In the present study, we investigated the effects of soyasaponin Ag, a secondary metabolite extracted from soybean, on α‑melanocyte-stimulating hormone (α‑MSH)‑induced melanin synthesis in B16F10 mouse melanoma cells and the underlying molecular mechanisms. To elucidate the mechanisms through which soyasaponin Ag inhibits melanin synthesis, we performed cellular tyrosinase activity assays and analyzed the expression of the melanogenesis‑related genes, tyrosinase, tyrosinase‑related protein (TRP)‑1 and TRP‑2. We demonstrated that soyasaponin Ag inhibited α‑MSH‑induced melanin synthesis in melanoma cells. Of note, soyasaponin Ag had no inhibitory effect on intracellular tyrosinase activity. However, soyasaponin Ag inhibited TRP‑2 expression in a dose‑dependent manner. Therefore, the depigmenting effect of soyasaponin Ag may be due to the inhibition of tyrosinase expression or the enhancement of tyrosinase degradation. Moreover, soyasaponin Ag did not exert any toxic on B16F10 mouse melanoma cells, suggesting that soyasaponin is a safe component for use in skin care cosmetic formulations that are used for skin whitening.

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