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Comparison of gene expression profiles between dental pulp and periodontal ligament tissues in humans

  • Authors:
    • Ai-Xiu Gong
    • Jing-Han Zhang
    • Jing Li
    • Jun Wu
    • Lin Wang
    • Deng-Shun Miao
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    Affiliations: Department of Stomatology, Children's Hospital of Nanjing Medical University, Nanjing, Jiangsu 210008, P.R. China, Department of Neonatology, Children's Hospital of Nanjing Medical University, Nanjing, Jiangsu 210008, P.R. China, State Key Laboratory of Reproductive Medicine, The Research Center for Bone and Stem Cells, Department of Anatomy, Histology and Embryology, Nanjing Medical University, Nanjing, Jiangsu 210029, P.R. China, Jiangsu Key Laboratory of Oral Diseases, Department of Orthodontics, School of Stomatology, Nanjing Medical University, Nanjing, Jiangsu 210029, P.R. China
    Copyright: © Gong et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 647-660
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    Published online on: July 12, 2017
       https://doi.org/10.3892/ijmm.2017.3065
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Abstract

There are anatomical and functional differences between human dental pulp (DP) and periodontal ligament (PDL). However, the molecular biological differences and function of these tissues are poorly understood. In the present study, we employed a cDNA microarray array to screen for differentially expressed genes (DEGs) between human DP and PDL tissues, and used the online software WebGestalt to perform the functional analysis of the DEGs. In addition, the STRING database and KEGG pathway analysis were applied for interaction network and pathway analysis of the DEGs. DP and PDL samples were obtained from permanent premolars (n=16) extracted for orthodontic purposes. The results of the microarray assay were confirmed by RT-qPCR. The DEGs were found to be significantly associated with the extracellular matrix and focal adhesion. A total of 10 genes were selected to confirm the results. The mRNA levels of integrin alpha 4 (ITGA4), integrin alpha 8 (ITGA8), neurexin 1 (NRXN1) and contactin 1 (CNTN1) were significantly higher in the DP than in the PDL tissues. However, the levels of collagen type XI alpha 1 (COL11A1), aggrecan (ACAN), collagen type VI alpha 1 (COL6A1), chondroadherin (CHAD), laminin gamma 2 (LAMC2) and laminin alpha 3 (LAMA3) were higher in the PDL than in the DP samples. The gene expression profiles provide novel insight into the characterization of DP and PDL tissues, and contribute to our understanding of the potential molecular mechanisms of dental tissue mineralization and regeneration.
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Copy and paste a formatted citation
Spandidos Publications style
Gong A, Zhang J, Li J, Wu J, Wang L and Miao D: Comparison of gene expression profiles between dental pulp and periodontal ligament tissues in humans. Int J Mol Med 40: 647-660, 2017.
APA
Gong, A., Zhang, J., Li, J., Wu, J., Wang, L., & Miao, D. (2017). Comparison of gene expression profiles between dental pulp and periodontal ligament tissues in humans. International Journal of Molecular Medicine, 40, 647-660. https://doi.org/10.3892/ijmm.2017.3065
MLA
Gong, A., Zhang, J., Li, J., Wu, J., Wang, L., Miao, D."Comparison of gene expression profiles between dental pulp and periodontal ligament tissues in humans". International Journal of Molecular Medicine 40.3 (2017): 647-660.
Chicago
Gong, A., Zhang, J., Li, J., Wu, J., Wang, L., Miao, D."Comparison of gene expression profiles between dental pulp and periodontal ligament tissues in humans". International Journal of Molecular Medicine 40, no. 3 (2017): 647-660. https://doi.org/10.3892/ijmm.2017.3065
Copy and paste a formatted citation
x
Spandidos Publications style
Gong A, Zhang J, Li J, Wu J, Wang L and Miao D: Comparison of gene expression profiles between dental pulp and periodontal ligament tissues in humans. Int J Mol Med 40: 647-660, 2017.
APA
Gong, A., Zhang, J., Li, J., Wu, J., Wang, L., & Miao, D. (2017). Comparison of gene expression profiles between dental pulp and periodontal ligament tissues in humans. International Journal of Molecular Medicine, 40, 647-660. https://doi.org/10.3892/ijmm.2017.3065
MLA
Gong, A., Zhang, J., Li, J., Wu, J., Wang, L., Miao, D."Comparison of gene expression profiles between dental pulp and periodontal ligament tissues in humans". International Journal of Molecular Medicine 40.3 (2017): 647-660.
Chicago
Gong, A., Zhang, J., Li, J., Wu, J., Wang, L., Miao, D."Comparison of gene expression profiles between dental pulp and periodontal ligament tissues in humans". International Journal of Molecular Medicine 40, no. 3 (2017): 647-660. https://doi.org/10.3892/ijmm.2017.3065
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