Open Access

Paeoniflorin exerts neuroprotective effects against glutamate‑induced PC12 cellular cytotoxicity by inhibiting apoptosis

  • Authors:
    • Ahong Chen
    • Hongyun Wang
    • Yuqin Zhang
    • Xiaoying Wang
    • Lishuang Yu
    • Wen Xu
    • Wei Xu
    • Yu Lin
  • View Affiliations

  • Published online on: July 20, 2017     https://doi.org/10.3892/ijmm.2017.3076
  • Pages: 825-833
  • Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Paeoniflorin (PF) is an active ingredient of Radix Paeoniae, which is known to exert neuroprotective effects. However, the mechanims behind the neuroprotective effects of PF are not yet fully understood. The apoptosis of neurons plays an important role in the cerebral ischemia-induced cascade response. This study aimed to investigate neuroprotective effects of PF against glutamate‑induced PC12 cellular cytotoxicity and to determine whether these effects are mediated via the inhibition of apoptosis in vitro and the activity of mitochondrial apoptosis-associated proteins in PC12 cells. Exposure of the PC12 cells to glutamate induced cell morphological changes, significantly decreased cell viability and induced apoptosis, with similar results being observed from the Hoechst 33342 staining and Annexin V/PI staining experiments. Glutamate also increased the lactate dehydrogenase release by the PC12 cells. However, treatment with PF prevented these effects. Furthermore, PF inhibited Bax and Bad expression and increased Bcl-2 and Bcl-xL expression; it also decreased the levels of downstream protein (caspase-3 and caspase-9). Collectively, our results indicate that PF protects PC12 cells against glutamate-induced neurotoxicity possibly through the inhibition of the expression of mitochondrial apoptosis-associated proteins.
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September-2017
Volume 40 Issue 3

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Chen A, Wang H, Zhang Y, Wang X, Yu L, Xu W, Xu W and Lin Y: Paeoniflorin exerts neuroprotective effects against glutamate‑induced PC12 cellular cytotoxicity by inhibiting apoptosis. Int J Mol Med 40: 825-833, 2017
APA
Chen, A., Wang, H., Zhang, Y., Wang, X., Yu, L., Xu, W. ... Lin, Y. (2017). Paeoniflorin exerts neuroprotective effects against glutamate‑induced PC12 cellular cytotoxicity by inhibiting apoptosis. International Journal of Molecular Medicine, 40, 825-833. https://doi.org/10.3892/ijmm.2017.3076
MLA
Chen, A., Wang, H., Zhang, Y., Wang, X., Yu, L., Xu, W., Xu, W., Lin, Y."Paeoniflorin exerts neuroprotective effects against glutamate‑induced PC12 cellular cytotoxicity by inhibiting apoptosis". International Journal of Molecular Medicine 40.3 (2017): 825-833.
Chicago
Chen, A., Wang, H., Zhang, Y., Wang, X., Yu, L., Xu, W., Xu, W., Lin, Y."Paeoniflorin exerts neuroprotective effects against glutamate‑induced PC12 cellular cytotoxicity by inhibiting apoptosis". International Journal of Molecular Medicine 40, no. 3 (2017): 825-833. https://doi.org/10.3892/ijmm.2017.3076