Grifola frondosa polysaccharides induce breast cancer cell apoptosis via the mitochondrial-dependent apoptotic pathway Corrigendum in /10.3892/ijmm.2022.5192

Zhang,Yizhi; Sun,Dejun; Meng,Qingjin; Guo,Wanxu; Chen,Qiuhui; Zhang,Ying

doi:10.3892/ijmm.2017.3081

Grifola frondosa polysaccharides induce breast cancer cell apoptosis via the mitochondrial-dependent apoptotic pathway

Corrigendum in: /10.3892/ijmm.2022.5192

Authors:
- Yizhi Zhang
- Dejun Sun
- Qingjin Meng
- Wanxu Guo
- Qiuhui Chen
- Ying Zhang
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Affiliations: Department of Neurology, The Second Hospital of Jilin University, Jilin University, Changchun, Jilin 130041, P.R. China, Department of Biomedicine, Institute for Regeneration Medicine, Jilin University, Changchun, Jilin 130021, P.R. China, Department of Neurology, Brain Hospital of Jilin Province, Siping, Jilin 136000, P.R. China
Published online on: July 26, 2017 https://doi.org/10.3892/ijmm.2017.3081
Pages: 1089-1095
Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Grifola frondosa, a type of food and medical fungus, has been shown to exhibit various pharmacological activities, including anticancer effects. As the most typical cancer diagnosed among female patients, breast cancer remains a huge concern threatening human health globally. In the present study, the anti-breast cancer effects of Grifola frondosa polysaccharides (GFPs) and the underlying mechanisms were investigated in MCF-7 and MDA-MB-231 cells, as well as in nude mice bearing MCF-7 tumor xenografts. GFPs exerted cytotoxic effects on the cells, as indicated by a decrease in cell viability, and an increase in the apoptototic rate, lactate dehydrogenase release and reactive oxygen species accumulation, inducing mitochondrial dysfunction. The increased expression of Bax, cleaved caspase-3 and caspase-8, and the reduced levels of B-cell lymphoma 2 (Bcl-2) and Bcl-extra large (Bcl‑xL) were observed in the cells incubated with GFPs and in the tumor tissues of the mice treated with GFPs. Moreover, the GFPs significantly suppressed the phosphorylation of AKT/glycogen synthase kinase-3β and extracellular signal-regulated kinases in a time-dependent manner. Finally, the inhibition of MCF-7 tumor xenograft growth further confirmed the anti-breast cancer effects of GFPs. All these findings revealed that GFPs induced human breast cancer cell apoptosis via the mitochondrial-dependent apoptotic pathway, and provide experimental evidence to support the use of Grifola frondosa as a potential treatment for breast cancer.

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