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Article

Triticum aestivum sprout-derived polysaccharide exerts hepatoprotective effects against ethanol-induced liver damage by enhancing the antioxidant system in mice

  • Authors:
    • Sarmila Nepali
    • Hyeon-Hui Ki
    • Ji-Hyun Lee
    • Ji-Yun Cha
    • Young-Mi Lee
    • Dae-Ki Kim
  • View Affiliations / Copyright

    Affiliations: Department of Immunology and Institute of Medical Sciences, Medical School, Chonbuk National University, Jeonju, Jeonbuk 54907, Republic of Korea, Department of Oriental Pharmacy, College of Pharmacy and Wonkwang-Oriental Medicine Research Institute, Wonkwang University, Iksan, Jeonbuk 54538, Republic of Korea
  • Pages: 1243-1252
    |
    Published online on: August 11, 2017
       https://doi.org/10.3892/ijmm.2017.3095
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Abstract

Triticum aestivum sprout-derived polysaccharide (TASP) has anti-diabetic properties, but no information is available in regards to its protective effect against ethanol-induced hepatic injury. This study aimed to investigate the mechanism behind the protective role of TASP against ethanol-induced liver injury in vivo. Male C57BL/6 mice were administered ethanol with or without TASP for 10 consecutive days by oral gavage. Silymarin was administered in the same manner as a positive control. TASP reduced ethanol-induced hepatic lipid accumulation and serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels. TASP also prevented glutathione (GSH) depletion and increased the superoxide dismutase (SOD) in liver tissue. In addition, TASP significantly inhibited ethanol-induced cytochrome P450 2E1 (CYP2E1) activation, and upregulated the expressions of nuclear factor erythroid 2-related factor 2 (Nrf2) and hemeoxygenase-1 (HO-1), and downregulated NADPH oxidase genes in ethanol fed mice. Furthermore, the upregulation of Nrf2 was found to be regulated by a phosphatidylinositol 3-kinase (PI3K)/Akt pathway. TASP also attenuated hepatic injury by modulation of caspase-3 and apoptosis-associated mitochondrial proteins including B-cell lymphoma-2 (Bcl-2) and Bcl-2-associated X (Bax) in liver tissues of mice. The study demonstrated that TASP treatment protects against ethanol-induced hepatic injury via multiple pathways by inhibiting steatosis and improving antioxidant marker levels during hepatic injury. Such properties provide a basis for therapeutic agents against alcohol-induced liver injury.
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Copy and paste a formatted citation
Spandidos Publications style
Nepali S, Ki H, Lee J, Cha J, Lee Y and Kim D: Triticum aestivum sprout-derived polysaccharide exerts hepatoprotective effects against ethanol-induced liver damage by enhancing the antioxidant system in mice. Int J Mol Med 40: 1243-1252, 2017.
APA
Nepali, S., Ki, H., Lee, J., Cha, J., Lee, Y., & Kim, D. (2017). Triticum aestivum sprout-derived polysaccharide exerts hepatoprotective effects against ethanol-induced liver damage by enhancing the antioxidant system in mice. International Journal of Molecular Medicine, 40, 1243-1252. https://doi.org/10.3892/ijmm.2017.3095
MLA
Nepali, S., Ki, H., Lee, J., Cha, J., Lee, Y., Kim, D."Triticum aestivum sprout-derived polysaccharide exerts hepatoprotective effects against ethanol-induced liver damage by enhancing the antioxidant system in mice". International Journal of Molecular Medicine 40.4 (2017): 1243-1252.
Chicago
Nepali, S., Ki, H., Lee, J., Cha, J., Lee, Y., Kim, D."Triticum aestivum sprout-derived polysaccharide exerts hepatoprotective effects against ethanol-induced liver damage by enhancing the antioxidant system in mice". International Journal of Molecular Medicine 40, no. 4 (2017): 1243-1252. https://doi.org/10.3892/ijmm.2017.3095
Copy and paste a formatted citation
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Spandidos Publications style
Nepali S, Ki H, Lee J, Cha J, Lee Y and Kim D: Triticum aestivum sprout-derived polysaccharide exerts hepatoprotective effects against ethanol-induced liver damage by enhancing the antioxidant system in mice. Int J Mol Med 40: 1243-1252, 2017.
APA
Nepali, S., Ki, H., Lee, J., Cha, J., Lee, Y., & Kim, D. (2017). Triticum aestivum sprout-derived polysaccharide exerts hepatoprotective effects against ethanol-induced liver damage by enhancing the antioxidant system in mice. International Journal of Molecular Medicine, 40, 1243-1252. https://doi.org/10.3892/ijmm.2017.3095
MLA
Nepali, S., Ki, H., Lee, J., Cha, J., Lee, Y., Kim, D."Triticum aestivum sprout-derived polysaccharide exerts hepatoprotective effects against ethanol-induced liver damage by enhancing the antioxidant system in mice". International Journal of Molecular Medicine 40.4 (2017): 1243-1252.
Chicago
Nepali, S., Ki, H., Lee, J., Cha, J., Lee, Y., Kim, D."Triticum aestivum sprout-derived polysaccharide exerts hepatoprotective effects against ethanol-induced liver damage by enhancing the antioxidant system in mice". International Journal of Molecular Medicine 40, no. 4 (2017): 1243-1252. https://doi.org/10.3892/ijmm.2017.3095
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