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Integrating microRNA and messenger RNA expression profiles in a rat model of deep vein thrombosis

  • Authors:
    • Qian-Qian Jin
    • Jun-Hong Sun
    • Qiu-Xiang Du
    • Xiao-Jun Lu
    • Xi-Yan Zhu
    • Hao-Liang Fan
    • Christian Hölscher
    • Ying-Yuan Wang
  • View Affiliations / Copyright

    Affiliations: Department of Forensic Pathology, Shanxi Medical University, Taiyuan, Shanxi 030001, P.R. China, Biochemical and Life Sciences, Lancaster University, Lancaster, Lancashire LA1 4YQ, UK
    Copyright: © Jin et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1019-1028
    |
    Published online on: August 23, 2017
       https://doi.org/10.3892/ijmm.2017.3105
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Abstract

Deep vein thrombosis (DVT) is a disease involving multiple genes and systems. MicroRNAs (miRNAs) represent a class of non-coding small RNAs that post-transcriptionally suppress their target genes. The expression patterns of miRNA and messenger RNA (mRNA) in DVT remain poorly characterized. The aim of the present study was to evaluate miRNA and mRNA expression profiles in a stasis-induced DVT rat model. Male SD rats were randomly divided into three groups as follows: DVT, sham and control. The inferior vena cava (IVC) of rats was ligated to construct stasis-induced DVT models. Rats were sacrificed three days after ligation, and morphological changes in the vein tissues were observed by hematoxylin and eosin and Masson staining. The miRNA and mRNA expression profiles were evaluated by microarrays, followed by bioinformatics analysis. The microarray analysis identified 22 miRNAs and 487 mRNAs that were significantly differentially expressed between the experimental and control groups, and between the experimental and sham groups, but not between the control and sham groups (P≤0.05; ≥2.0‑fold change). By subsequent bioinformatics analysis, a 19 miRNA-98 mRNAs network was constructed in the stasis-induced DVT rat model. Notably, the majority of these miRNAs and mRNAs are reported to be expressed by endothelial cells (ECs) and are associated with the function of ECs. The results provide evidence indicating that the regulatory association of miRNA and mRNA points to key roles played by ECs in thrombosis. These findings advance our understanding of the molecular regulatory mechanisms underlying the pathophysiology of DVT.
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Copy and paste a formatted citation
Spandidos Publications style
Jin Q, Sun J, Du Q, Lu X, Zhu X, Fan H, Hölscher C and Wang Y: Integrating microRNA and messenger RNA expression profiles in a rat model of deep vein thrombosis. Int J Mol Med 40: 1019-1028, 2017.
APA
Jin, Q., Sun, J., Du, Q., Lu, X., Zhu, X., Fan, H. ... Wang, Y. (2017). Integrating microRNA and messenger RNA expression profiles in a rat model of deep vein thrombosis. International Journal of Molecular Medicine, 40, 1019-1028. https://doi.org/10.3892/ijmm.2017.3105
MLA
Jin, Q., Sun, J., Du, Q., Lu, X., Zhu, X., Fan, H., Hölscher, C., Wang, Y."Integrating microRNA and messenger RNA expression profiles in a rat model of deep vein thrombosis". International Journal of Molecular Medicine 40.4 (2017): 1019-1028.
Chicago
Jin, Q., Sun, J., Du, Q., Lu, X., Zhu, X., Fan, H., Hölscher, C., Wang, Y."Integrating microRNA and messenger RNA expression profiles in a rat model of deep vein thrombosis". International Journal of Molecular Medicine 40, no. 4 (2017): 1019-1028. https://doi.org/10.3892/ijmm.2017.3105
Copy and paste a formatted citation
x
Spandidos Publications style
Jin Q, Sun J, Du Q, Lu X, Zhu X, Fan H, Hölscher C and Wang Y: Integrating microRNA and messenger RNA expression profiles in a rat model of deep vein thrombosis. Int J Mol Med 40: 1019-1028, 2017.
APA
Jin, Q., Sun, J., Du, Q., Lu, X., Zhu, X., Fan, H. ... Wang, Y. (2017). Integrating microRNA and messenger RNA expression profiles in a rat model of deep vein thrombosis. International Journal of Molecular Medicine, 40, 1019-1028. https://doi.org/10.3892/ijmm.2017.3105
MLA
Jin, Q., Sun, J., Du, Q., Lu, X., Zhu, X., Fan, H., Hölscher, C., Wang, Y."Integrating microRNA and messenger RNA expression profiles in a rat model of deep vein thrombosis". International Journal of Molecular Medicine 40.4 (2017): 1019-1028.
Chicago
Jin, Q., Sun, J., Du, Q., Lu, X., Zhu, X., Fan, H., Hölscher, C., Wang, Y."Integrating microRNA and messenger RNA expression profiles in a rat model of deep vein thrombosis". International Journal of Molecular Medicine 40, no. 4 (2017): 1019-1028. https://doi.org/10.3892/ijmm.2017.3105
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