Particulate matter 2.5 regulates lipid synthesis and inflammatory cytokine production in human SZ95 sebocytes

Liu,Qin; Wu,Jianbo; Song,Jiquan; Liang,Pin; Zheng,Kaiping; Xiao,Guifeng; Liu,Lanting; Zouboulis,Christos  C.; Lei,Tiechi

doi:10.3892/ijmm.2017.3109

Particulate matter 2.5 regulates lipid synthesis and inflammatory cytokine production in human SZ95 sebocytes

Authors:
- Qin Liu
- Jianbo Wu
- Jiquan Song
- Pin Liang
- Kaiping Zheng
- Guifeng Xiao
- Lanting Liu
- Christos C. Zouboulis
- Tiechi Lei
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Affiliations: Department of Dermatology, Renmin Hospital of Wuhan University, Wuchang, Wuhan, Hubei 430060, P.R. China, Department of Dermatology, Zhongnan Hospital of Wuhan University, Wuchang, Wuhan, Hubei 430071, P.R. China, Department of Dermatology, Venereology, Allergology and Immunology, Dessau Medical Center, Theodore Fontane Medical University of Brandenburg, Dessau D-06847, Germany
Published online on: August 25, 2017 https://doi.org/10.3892/ijmm.2017.3109
Pages: 1029-1036
Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

A large body of evidence indicates that particulate matter (PM)2.5 is associated with various negative effects on human health. However, the impact and molecular mechanism of PM2.5 on the skin have not been elucidated. Therefore, the present study aimed to investigate the effects of two types of PM2.5 [water-soluble extracts (W-PM2.5) and non-water-soluble extracts (NW-PM2.5)] on cell proliferation, cell cycle progression, lipid synthesis, and inflammatory cytokine production of human SZ95 sebocytes. The results demonstrated that NW-PM2.5 and W-PM2.5 exposure dose-dependently inhibited SZ95 sebocyte proliferation by inducing G1 cell arrest. Furthermore, NW-PM2.5 and W-PM2.5 significantly reduced sebaceous lipid synthesis and markedly promoted the production of inflammatory cytokines, including interleukin-1α (IL-1α), IL-6 and IL-8 in SZ95 sebocytes. Additionally, the expression of aryl hydrocarbon (Ah) receptor (AhR), AhR nuclear translocator protein (ARNT), as well as cytochrome P450 1A1 were significantly increased following PM2.5 exposure. Thus, these findings indicate that PM2.5 exerts inhibitory effects on cell proliferation and lipid synthesis, and stimulatory effects on inflammatory cytokine production and AhR signaling activation in human SZ95 sebocytes.

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1	Kim KE, Cho D and Park HJ: Air pollution and skin diseases: Adverse effects of airborne particulate matter on various skin diseases. Life Sci. 152:126–134. 2016. View Article : Google Scholar : PubMed/NCBI
2	Krutmann J, Liu W, Li L, Pan X, Crawford M, Sore G and Seite S: Pollution and skin: From epidemiological and mechanistic studies to clinical implications. J Dermatol Sci. 76:163–168. 2014. View Article : Google Scholar : PubMed/NCBI
3	Ju Q, Zouboulis CC and Xia L: Environmental pollution and acne: Chloracne. Dermatoendocrinol. 1:125–128. 2009. View Article : Google Scholar
4	Ju Q and Zouboulis CC: Endocrine-disrupting chemicals and skin manifestations. Rev Endocr Metab Disord. 17:449–457. 2016. View Article : Google Scholar : PubMed/NCBI
5	Ju Q, Fimmel S, Hinz N, Stahlmann R, Xia L and Zouboulis CC: 2,3,7,8-Tetrachlorodibenzo-p-dioxin alters sebaceous gland cell differentiation in vitro. Exp Dermatol. 20:320–325. 2011. View Article : Google Scholar : PubMed/NCBI
6	Hu T, Pan Z, Yu Q, Mo X, Song N, Yan M, Zouboulis CC, Xia L and Ju Q: Benzo(a)pyrene induces interleukin (IL)-6 production and reduces lipid synthesis in human SZ95 sebocytes via the aryl hydrocarbon receptor signaling pathway. Environ Toxicol Pharmacol. 43:54–60. 2016. View Article : Google Scholar : PubMed/NCBI
7	Ju Q, Yiang K, Zouboulis CC, Ring J and Chen W: Chloracne: From clinic to research. Zhonghua Pifuke Yixue Zazhi. 30:2–6. 2012.
8	Díaz RV and Rosa Dominguez E: Health risk by inhalation of PM2.5 in the metropolitan zone of the City of Mexico. Ecotoxicol Environ Saf. 72:866–871. 2009. View Article : Google Scholar
9	Dockery DW: Health effects of particulate air pollution. Ann Epidemiol. 19:257–263. 2009. View Article : Google Scholar : PubMed/NCBI
10	Weichenthal SA, Godri-Pollitt K and Villeneuve PJ: PM2.5, oxidant defence and cardiorespiratory health: A review. Environ Health. 12:402013. View Article : Google Scholar : PubMed/NCBI
11	Li Q, Kang Z, Jiang S, Zhao J, Yan S, Xu F and Xu J: Effects of ambient fine particles PM2.5 on human HaCaT cells. Int J Environ Res Public Health. 14:E722017. View Article : Google Scholar
12	Sugihara K, Kitamura S, Yamada T, Ohta S, Yamashita K, Yasuda M and Fujii-Kuriyama Y: Aryl hydrocarbon receptor (AhR)-mediated induction of xanthine oxidase/xanthine dehydrogenase activity by 2,3,7,8-tetrachlorodibenzo-p-dioxin. Biochem Biophys Res Commun. 281:1093–1099. 2001. View Article : Google Scholar : PubMed/NCBI
13	Hong CH, Lee CH, Yu HS and Huang SK: Benzopyrene, a major polyaromatic hydrocarbon in smoke fume, mobilizes Langerhans cells and polarizes Th2/17 responses in epicutaneous protein sensitization through the aryl hydrocarbon receptor. Int Immunopharmacol. 36:111–117. 2016. View Article : Google Scholar : PubMed/NCBI
14	Jux B, Kadow S and Esser C: Langerhans cell maturation and contact hypersensitivity are impaired in aryl hydrocarbon receptor-null mice. J Immunol. 182:6709–6717. 2009. View Article : Google Scholar : PubMed/NCBI
15	Cho YC, Zheng W and Jefcoate CR: Disruption of cell-cell contact maximally but transiently activates AhR-mediated transcription in 10T1/2 fibroblasts. Toxicol Appl Pharmacol. 199:220–238. 2004. View Article : Google Scholar : PubMed/NCBI
16	Hu T, Wang D, Yu Q, Li L, Mo X, Pan Z, Zouboulis CC, Peng L, Xia L and Ju Q: Aryl hydrocarbon receptor negatively regulates lipid synthesis and involves in cell differentiation of SZ95 sebocytes in vitro. Chem Biol Interact. 258:52–58. 2016. View Article : Google Scholar : PubMed/NCBI
17	Ju Q, Yu Q, Song N, Tan Y, Xia L and Zouboulis CC: Expression of aryl hydrocarbon receptor in human epidermis, hair follicles and sebaceous glands and its significance. Zhonghua Pifuke Zazhi. 44:761–764. 2011.
18	Esser C, Bargen I, Weighardt H, Haarmann-Stemmann T and Krutmann J: Functions of the aryl hydrocarbon receptor in the skin. Semin Immunopathol. 35:677–691. 2013. View Article : Google Scholar : PubMed/NCBI
19	Yu Q, Hu T, Mo X, Zhang C, Xia L, Zouboulis CC and Ju Q: Effect of tetrachlorodibenzo-p-dioxin on the expression of cytochrome P4501A1 in human SZ95 sebocytes and its significance. Zhonghua Pifuke Zazhi. 46:557–560. 2013.
20	Kalmes M, Hennen J, Clemens J and Blömeke B: Impact of aryl hydrocarbon receptor (AhR) knockdown on cell cycle progression in human HaCaT keratinocytes. Biol Chem. 392:643–651. 2011. View Article : Google Scholar : PubMed/NCBI
21	Tauchi M, Hida A, Negishi T, Katsuoka F, Noda S, Mimura J, Hosoya T, Yanaka A, Aburatani H, Fujii-Kuriyama Y, et al: Constitutive expression of aryl hydrocarbon receptor in keratinocytes causes inflammatory skin lesions. Mol Cell Biol. 25:9360–9368. 2005. View Article : Google Scholar : PubMed/NCBI
22	Kakimoto K, Nagayoshi H, Konishi Y, Kajimura K, Ohura T, Nakano T, Hata M, Furuuchi M, Tang N, Hayakawa K, et al: Size distribution of chlorinated polycyclic aromatic hydrocarbons in atmospheric particles. Arch Environ Contam Toxicol. 72:58–64. 2017. View Article : Google Scholar
23	Jeong SC, Song MK, Cho Y, Lee E and Ryu JC: Integrative analysis of mRNA and microRNA expression of a human alveolar epithelial cell(A549) exposed to water and organic-soluble extract from particulate matter (PM)2.5. Environ Toxicol. 32:302–310. 2017. View Article : Google Scholar
24	Yang B, Chen D, Zhao H and Xiao C: The effects for PM2.5 exposure on non-small-cell lung cancer induced motility and proliferation. Springerplus. 5:20592016. View Article : Google Scholar : PubMed/NCBI
25	Zhou Z, Liu Y, Duan F, Qin M, Wu F, Sheng W, Yang L, Liu J and He K: Transcriptomic analyses of the biological effects of airborne PM2.5 exposure on human bronchial epithelial cells. PLoS One. 10:e01382672015. View Article : Google Scholar : PubMed/NCBI
26	Huang Q, Zhang J, Peng S, Tian M, Chen J and Shen H: Effects of water soluble PM2.5 extracts exposure on human lung epithelial cells (A549): A proteomic study. J Appl Toxicol. 34:675–687. 2014. View Article : Google Scholar
27	Zhang J, Ghio AJ, Gao M, Wei K, Rosen GD and Upadhyay D: Ambient particulate matter induces alveolar epithelial cell cycle arrest: Role of G cyclins. FEBS Lett. 581:5315–5320. 2007. View Article : Google Scholar : PubMed/NCBI
28	Longhin E, Holme JA, Gutzkow KB, Arlt VM, Kucab JE, Camatini M and Gualtieri M: Cell cycle alterations induced by urban PM2.5 in bronchial epithelial cells: Characterization of the process and possible mechanisms involved. Part Fibre Toxicol. 10:632013. View Article : Google Scholar : PubMed/NCBI
29	Chen W, Obermayer-Pietsch B, Hong J-B, Melnik BC, Yamasaki O, Dessinioti C, Ju Q, Liakou AI, Al-Khuzaei S, Katsambas A, et al: Acne-associated syndromes: Models for better understanding of acne pathogenesis. J Eur Acad Dermatol Venereol. 25:637–646. 2011. View Article : Google Scholar : PubMed/NCBI
30	Zouboulis CC, Picardo M, Ju Q, Kurokawa I, Törőcsik D, Bíró T and Schneider MR: Beyond acne: Current aspects of sebaceous gland biology and function. Rev Endocr Metab Disord. 17:319–334. 2016. View Article : Google Scholar : PubMed/NCBI
31	Zouboulis CC, Jourdan E and Picardo M: Acne is an inflammatory disease and alterations of sebum composition initiate acne lesions. J Eur Acad Dermatol Venereol. 28:527–532. 2014. View Article : Google Scholar
32	Ganceviciene R, Graziene V, Fimmel S and Zouboulis CC: Involvement of the corticotropin-releasing hormone system in the pathogenesis of acne vulgaris. Br J Dermatol. 160:345–352. 2009. View Article : Google Scholar
33	Zhang H, Yao Y, Chen Y, Yue C, Chen J, Tong J, Jiang Y and Chen T: Crosstalk between AhR and wnt/β-catenin signal pathways in the cardiac developmental toxicity of PM2.5 in zebrafish embryos. Toxicology. 355–356:31–38. 2016. View Article : Google Scholar
34	Borgie M, Ledoux F, Verdin A, Cazier F, Greige H, Shirali P, Courcot D and Dagher Z: Genotoxic and epigenotoxic effects of fine particulate matter from rural and urban sites in Lebanon on human bronchial epithelial cells. Environ Res. 136:352–362. 2015. View Article : Google Scholar
35	Líbalová H, Krčková S, Uhlířová K, Milcová A, Schmuczerová J, Ciganek M, Kléma J, Machala M, Šrám RJ and Topinka J: Genotoxicity but not the AhR-mediated activity of PAHs is inhibited by other components of complex mixtures of ambient air pollutants. Toxicol Lett. 225:350–357. 2014. View Article : Google Scholar : PubMed/NCBI