Open Access

Analysis of dermal fibroblasts isolated from neonatal and child cleft lip and adult skin: Developmental implications on reconstructive surgery

  • Authors:
    • Veronika Živicová
    • Lukáš Lacina
    • Rosana Mateu
    • Karel Smetana
    • Radana Kavková
    • Eliška Drobná Krejčí
    • Miloš Grim
    • Alena Kvasilová
    • Jiří Borský
    • Hynek Strnad
    • Miluše Hradilová
    • Jana Šáchová
    • Michal Kolář
    • Barbora Dvořánková
  • View Affiliations

  • Published online on: September 7, 2017     https://doi.org/10.3892/ijmm.2017.3128
  • Pages: 1323-1334
  • Copyright: © Živicová et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

The nonsyndromic cleft is one of the most frequent congenital defects in humans. Clinical data demonstrated improved and almost scarless neonatal healing of reparative surgery. Based on our previous results on crosstalk between neonatal fibroblasts and adult keratinocytes, the present study focused on characterization of fibroblasts prepared from cleft lip tissue samples of neonates and older children, and compared them with samples isolated from normal adult skin (face and breast) and scars. Although subtle variances in expression profiles of children and neonates were observed, the two groups differed significantly from adult cells. Compared with adult cells, differences were observed in nestin and smooth muscle actin (SMA) expression at the protein and transcript level. Furthermore, fibroblast to myofibroblast differentiation drives effective wound healing and is largely regulated by the cytokine, transforming growth factor-β1 (TGF-β1). Dysregulation of the TGF-β signalling pathway, including low expression of the TGF-β receptor II, may contribute to reducing scarring in neonates. Fibroblasts of facial origin also exhibited age independent differences from the cells prepared from the breast, reflecting the origin of the facial cells from neural crest-based ectomesenchyme.
View Figures
View References

Related Articles

Journal Cover

November-2017
Volume 40 Issue 5

Print ISSN: 1107-3756
Online ISSN:1791-244X

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Živicová V, Lacina L, Mateu R, Smetana K, Kavková R, Drobná Krejčí E, Grim M, Kvasilová A, Borský J, Strnad H, Strnad H, et al: Analysis of dermal fibroblasts isolated from neonatal and child cleft lip and adult skin: Developmental implications on reconstructive surgery. Int J Mol Med 40: 1323-1334, 2017
APA
Živicová, V., Lacina, L., Mateu, R., Smetana, K., Kavková, R., Drobná Krejčí, E. ... Dvořánková, B. (2017). Analysis of dermal fibroblasts isolated from neonatal and child cleft lip and adult skin: Developmental implications on reconstructive surgery. International Journal of Molecular Medicine, 40, 1323-1334. https://doi.org/10.3892/ijmm.2017.3128
MLA
Živicová, V., Lacina, L., Mateu, R., Smetana, K., Kavková, R., Drobná Krejčí, E., Grim, M., Kvasilová, A., Borský, J., Strnad, H., Hradilová, M., Šáchová, J., Kolář, M., Dvořánková, B."Analysis of dermal fibroblasts isolated from neonatal and child cleft lip and adult skin: Developmental implications on reconstructive surgery". International Journal of Molecular Medicine 40.5 (2017): 1323-1334.
Chicago
Živicová, V., Lacina, L., Mateu, R., Smetana, K., Kavková, R., Drobná Krejčí, E., Grim, M., Kvasilová, A., Borský, J., Strnad, H., Hradilová, M., Šáchová, J., Kolář, M., Dvořánková, B."Analysis of dermal fibroblasts isolated from neonatal and child cleft lip and adult skin: Developmental implications on reconstructive surgery". International Journal of Molecular Medicine 40, no. 5 (2017): 1323-1334. https://doi.org/10.3892/ijmm.2017.3128