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Article

Structure-activity relationship of three synthesized benzimidazole-based oligosaccharides in human platelet activation

Corrigendum in: /10.3892/ijmm.2019.4423
  • Authors:
    • Yi Chang
    • Wen-Hsien Hsu
    • Wen-Bin Yang
    • Thanasekaran Jayakumar
    • Tzu-Yin Lee
    • Joen-Rong Sheu
    • Wan-Jung Lu
    • Jiun-Yi Li
  • View Affiliations / Copyright

    Affiliations: Department of Anesthesiology, Shin Kong Wu Ho-Su Memorial Hospital, Taipei 111, Taiwan, R.O.C., School of Medicine, Fu-Jen Catholic University, Xin Zhuang, New Taipei City 242, Taiwan, R.O.C., Genomics Research Center, Academia Sinica, Taipei 115, Taiwan, R.O.C., Department of Pharmacology and Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei 110, Taiwan, R.O.C.
  • Pages: 1520-1528
    |
    Published online on: September 13, 2017
       https://doi.org/10.3892/ijmm.2017.3133
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Abstract

Antiplatelet agents have considerable benefits in the treatment of thromboembolic diseases; however, these agents still have substantial limitations due to their severe side-effects. In this study, the antiplatelet activity of three newly synthesized saccharide based benzimidazole derivatives, M3BIM, Malto-BIM and Melibio-BIM, in collagen and thrombin-stimulated human platelets in vitro was examined. Among the compounds tested, only compound M3BIM exerted concentration (20-60 µM)-dependent inhibitory effects against collagen (1 µg/ml) and thrombin (0.01 U/ml)-induced washed human platelet aggregation. Moreover, at a concentration of 60 µM, M3BIM distinctly abolished collagen-induced adenosine triphosphate (ATP) release and intracellular Ca2+ mobilization. Additionally, this compound attenuated the collagen-induced phosphorylation of p47, a marker of the activation of protein kinase C (PKC) and p38 mitogen-activated protein kinase (MAPK). However, Malto-BIM and Melibio-BIM were not effective in this regard. Moreover, the toxic effects of these compounds were evaluated using zebrafish embryo toxicity (ZET) assay, and the results revealed that all three compounds had no comparative cytotoxicity within the range of 25-200 µM. Overall, the results of this study provide evidence for the inhibitory effects of M3BIM on collagen-induced platelet aggregation in vitro compared to other imidazole derivatives. The presence of 1-imidazolyl moiety at one end with a longer chain length (three sugar moieties) may be mainly responsible for the observed effects of M3BIM. These results suggest that compound M3BIM may be used as a potential candidate for the treatment of aberrant platelet activation-related diseases as it inhibits the activation of p47 and p38 MAPK, and reduces ATP release and Ca2+ mobilization.
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Copy and paste a formatted citation
Spandidos Publications style
Chang Y, Hsu W, Yang W, Jayakumar T, Lee T, Sheu J, Lu W and Li J: Structure-activity relationship of three synthesized benzimidazole-based oligosaccharides in human platelet activation Corrigendum in /10.3892/ijmm.2019.4423. Int J Mol Med 40: 1520-1528, 2017.
APA
Chang, Y., Hsu, W., Yang, W., Jayakumar, T., Lee, T., Sheu, J. ... Li, J. (2017). Structure-activity relationship of three synthesized benzimidazole-based oligosaccharides in human platelet activation Corrigendum in /10.3892/ijmm.2019.4423. International Journal of Molecular Medicine, 40, 1520-1528. https://doi.org/10.3892/ijmm.2017.3133
MLA
Chang, Y., Hsu, W., Yang, W., Jayakumar, T., Lee, T., Sheu, J., Lu, W., Li, J."Structure-activity relationship of three synthesized benzimidazole-based oligosaccharides in human platelet activation Corrigendum in /10.3892/ijmm.2019.4423". International Journal of Molecular Medicine 40.5 (2017): 1520-1528.
Chicago
Chang, Y., Hsu, W., Yang, W., Jayakumar, T., Lee, T., Sheu, J., Lu, W., Li, J."Structure-activity relationship of three synthesized benzimidazole-based oligosaccharides in human platelet activation Corrigendum in /10.3892/ijmm.2019.4423". International Journal of Molecular Medicine 40, no. 5 (2017): 1520-1528. https://doi.org/10.3892/ijmm.2017.3133
Copy and paste a formatted citation
x
Spandidos Publications style
Chang Y, Hsu W, Yang W, Jayakumar T, Lee T, Sheu J, Lu W and Li J: Structure-activity relationship of three synthesized benzimidazole-based oligosaccharides in human platelet activation Corrigendum in /10.3892/ijmm.2019.4423. Int J Mol Med 40: 1520-1528, 2017.
APA
Chang, Y., Hsu, W., Yang, W., Jayakumar, T., Lee, T., Sheu, J. ... Li, J. (2017). Structure-activity relationship of three synthesized benzimidazole-based oligosaccharides in human platelet activation Corrigendum in /10.3892/ijmm.2019.4423. International Journal of Molecular Medicine, 40, 1520-1528. https://doi.org/10.3892/ijmm.2017.3133
MLA
Chang, Y., Hsu, W., Yang, W., Jayakumar, T., Lee, T., Sheu, J., Lu, W., Li, J."Structure-activity relationship of three synthesized benzimidazole-based oligosaccharides in human platelet activation Corrigendum in /10.3892/ijmm.2019.4423". International Journal of Molecular Medicine 40.5 (2017): 1520-1528.
Chicago
Chang, Y., Hsu, W., Yang, W., Jayakumar, T., Lee, T., Sheu, J., Lu, W., Li, J."Structure-activity relationship of three synthesized benzimidazole-based oligosaccharides in human platelet activation Corrigendum in /10.3892/ijmm.2019.4423". International Journal of Molecular Medicine 40, no. 5 (2017): 1520-1528. https://doi.org/10.3892/ijmm.2017.3133
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