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Phosphorylation of carboxypeptidase B1 protein regulates β-cell proliferation

  • Authors:
    • Seong-Lan Yu
    • Seungyun Han
    • Hong Rye Kim
    • Jong Woo Park
    • Dong Il Jin
    • Jaeku Kang
  • View Affiliations / Copyright

    Affiliations: Department of Pharmacology, College of Medicine, Konyang University, Daejeon 35365, Republic of Korea, Department of Anatomy, College of Medicine, Konyang University, Daejeon 35365, Republic of Korea, Department of Animal Science and Biotechnology, Chungnam National University, Daejeon 34134, Republic of Korea, Department of Pharmacology, SungKyunKwan University, Suwon 16419, Republic of Korea
    Copyright: © Yu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1397-1404
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    Published online on: September 19, 2017
       https://doi.org/10.3892/ijmm.2017.3141
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Abstract

A reduction in pancreatic islet β-cells leads to the onset of diabetes. Hence, the identification of the mechanisms inducing β-cell proliferation is important for developing a treatment course against the disease. It has been well established that post-translational modifications (PTMs) of proteins affect their functionality. In addition, PTMs have been suggested to play important roles in organ regeneration. Therefore, in this study, we investigated PTMs associated with pancreatic regeneration using two-dimensional electrophoresis. Four carboxypeptidase B1 (CPB1) proteins were identified at different isoelectric points, with the same molecular weight. The motif of CPB1 PTMs was identified by mass spectrophotometry, and the downregulation of CPB1 phosphorylation in pancreatectomy was confirmed. The dephosphorylation of CPB1 induced β-cell proliferation. We thus surmise that the altered PTM of CPB1 is associated with pancreatic regeneration.
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Copy and paste a formatted citation
Spandidos Publications style
Yu S, Han S, Kim H, Park J, Jin D and Kang J: Phosphorylation of carboxypeptidase B1 protein regulates β-cell proliferation. Int J Mol Med 40: 1397-1404, 2017.
APA
Yu, S., Han, S., Kim, H., Park, J., Jin, D., & Kang, J. (2017). Phosphorylation of carboxypeptidase B1 protein regulates β-cell proliferation. International Journal of Molecular Medicine, 40, 1397-1404. https://doi.org/10.3892/ijmm.2017.3141
MLA
Yu, S., Han, S., Kim, H., Park, J., Jin, D., Kang, J."Phosphorylation of carboxypeptidase B1 protein regulates β-cell proliferation". International Journal of Molecular Medicine 40.5 (2017): 1397-1404.
Chicago
Yu, S., Han, S., Kim, H., Park, J., Jin, D., Kang, J."Phosphorylation of carboxypeptidase B1 protein regulates β-cell proliferation". International Journal of Molecular Medicine 40, no. 5 (2017): 1397-1404. https://doi.org/10.3892/ijmm.2017.3141
Copy and paste a formatted citation
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Spandidos Publications style
Yu S, Han S, Kim H, Park J, Jin D and Kang J: Phosphorylation of carboxypeptidase B1 protein regulates β-cell proliferation. Int J Mol Med 40: 1397-1404, 2017.
APA
Yu, S., Han, S., Kim, H., Park, J., Jin, D., & Kang, J. (2017). Phosphorylation of carboxypeptidase B1 protein regulates β-cell proliferation. International Journal of Molecular Medicine, 40, 1397-1404. https://doi.org/10.3892/ijmm.2017.3141
MLA
Yu, S., Han, S., Kim, H., Park, J., Jin, D., Kang, J."Phosphorylation of carboxypeptidase B1 protein regulates β-cell proliferation". International Journal of Molecular Medicine 40.5 (2017): 1397-1404.
Chicago
Yu, S., Han, S., Kim, H., Park, J., Jin, D., Kang, J."Phosphorylation of carboxypeptidase B1 protein regulates β-cell proliferation". International Journal of Molecular Medicine 40, no. 5 (2017): 1397-1404. https://doi.org/10.3892/ijmm.2017.3141
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