Open Access

Phosphorylation of carboxypeptidase B1 protein regulates β-cell proliferation

  • Authors:
    • Seong-Lan Yu
    • Seungyun Han
    • Hong Rye Kim
    • Jong Woo Park
    • Dong Il Jin
    • Jaeku Kang
  • View Affiliations

  • Published online on: September 19, 2017     https://doi.org/10.3892/ijmm.2017.3141
  • Pages: 1397-1404
  • Copyright: © Yu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

A reduction in pancreatic islet β-cells leads to the onset of diabetes. Hence, the identification of the mechanisms inducing β-cell proliferation is important for developing a treatment course against the disease. It has been well established that post-translational modifications (PTMs) of proteins affect their functionality. In addition, PTMs have been suggested to play important roles in organ regeneration. Therefore, in this study, we investigated PTMs associated with pancreatic regeneration using two-dimensional electrophoresis. Four carboxypeptidase B1 (CPB1) proteins were identified at different isoelectric points, with the same molecular weight. The motif of CPB1 PTMs was identified by mass spectrophotometry, and the downregulation of CPB1 phosphorylation in pancreatectomy was confirmed. The dephosphorylation of CPB1 induced β-cell proliferation. We thus surmise that the altered PTM of CPB1 is associated with pancreatic regeneration.
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November-2017
Volume 40 Issue 5

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Yu S, Han S, Kim H, Park J, Jin D and Kang J: Phosphorylation of carboxypeptidase B1 protein regulates β-cell proliferation. Int J Mol Med 40: 1397-1404, 2017
APA
Yu, S., Han, S., Kim, H., Park, J., Jin, D., & Kang, J. (2017). Phosphorylation of carboxypeptidase B1 protein regulates β-cell proliferation. International Journal of Molecular Medicine, 40, 1397-1404. https://doi.org/10.3892/ijmm.2017.3141
MLA
Yu, S., Han, S., Kim, H., Park, J., Jin, D., Kang, J."Phosphorylation of carboxypeptidase B1 protein regulates β-cell proliferation". International Journal of Molecular Medicine 40.5 (2017): 1397-1404.
Chicago
Yu, S., Han, S., Kim, H., Park, J., Jin, D., Kang, J."Phosphorylation of carboxypeptidase B1 protein regulates β-cell proliferation". International Journal of Molecular Medicine 40, no. 5 (2017): 1397-1404. https://doi.org/10.3892/ijmm.2017.3141