Saponin-enriched extract of Asparagus cochinchinensis alleviates airway inflammation and remodeling in ovalbumin-induced asthma model

Sung,Ji-Eun; Lee,Hyun-Ah; Kim,Ji-Eun; Yun,Woo-Bin; An,Beum-Soo; Yang,Seung-Yun; Kim,Dong-Seob; Lee,Chung-Yeoul; Lee,Hee-Seob; Bae,Chang-Joon; Hwang,Dae-Youn

doi:10.3892/ijmm.2017.3147

Saponin-enriched extract of Asparagus cochinchinensis alleviates airway inflammation and remodeling in ovalbumin-induced asthma model

Authors:
- Ji-Eun Sung
- Hyun-Ah Lee
- Ji-Eun Kim
- Woo-Bin Yun
- Beum-Soo An
- Seung-Yun Yang
- Dong-Seob Kim
- Chung-Yeoul Lee
- Hee-Seob Lee
- Chang-Joon Bae
- Dae-Youn Hwang
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Affiliations: Department of Biomaterials Science, College of Natural Resources and Life Science/Life and Industry Convergence Research Institute, Pusan National University, Miryang 50463, Republic of Korea, Department of Food Science and Technlogy, College of Natural Resources and Life Science/Life and Industry Convergence Research Institute, Pusan National University, Miryang 50463, Republic of Korea, Gangrim Organics, Miryang 50455, Republic of Korea, Department of Food Science and Nutrition, College of Human Ecology, Pusan National University, Busan 46241, Republic of Korea, Biologics Division, Ministry of Food and Drug Safety, Cheongju 28159, Republic of Korea
Published online on: September 21, 2017 https://doi.org/10.3892/ijmm.2017.3147
Pages: 1365-1376
Copyright: © Sung et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Asthma is a chronic inflammatory disease characterized by T-lymphocyte and eosinophil infiltration, mucus overproduction and airway hyper-responsiveness. The present study examined the therapeutic effects and action mechanism of a saponin-enriched extract of Asparagus cochinchinensis (SEAC) on airway inflammation and remodeling in an ovalbumin (OVA)-induced asthma model. To accomplish this, alterations of the nitric oxide (NO) level, inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) expression levels, as well as variations in immune cell numbers, immunoglobulin E (IgE) concentration, histopathological structure and inflammatory cytokine levels were measured in lipopolysaccharide (LPS)-activated RAW264.7 cells or an OVA-induced mouse model of asthma treated with SEAC. The concentration of NO and mRNA levels of COX-2 and iNOS were significantly decreased in the SEAC + LPS-treated RAW264.7 cells compared with the vehicle + LPS-treated RAW264.7 cells. Additionally, in the OVA-induced asthma model, the number of immune cells in the bronchoalveolar lavage fluid, the concentration of OVA-specific IgE, the infiltration of inflammatory cells, the bronchial thickness and the levels of the inflammatory mediators interleukin-4 (IL-4), IL-13 and COX-2 were significantly lower in the OVA + SEAC‑treated group compared with the OVA + vehicle‑treated group. In addition, a significant reduction in goblet cell hyperplasia, peribronchiolar collagen layer thickness and VEGF expression for airway remodeling was detected in the OVA + SEAC‑treated group compared with the OVA + vehicle‑treated group. These findings indicate that SEAC is a suppressor of airway inflammation and remodeling, and may therefore be useful as an anti-inflammatory drug for the treatment of asthma.

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