Generation of human-induced pluripotent stem cells from burn patient-derived skin fibroblasts using a non-integrative method

Fu,Shangfeng; Ding,Jianwu; Liu,Dewu; Huang,Heping; Li,Min; Liu,Yang; Tu,Longxiang; Liu,Deming

doi:10.3892/ijmm.2017.3206

Generation of human-induced pluripotent stem cells from burn patient-derived skin fibroblasts using a non-integrative method

Authors:
- Shangfeng Fu
- Jianwu Ding
- Dewu Liu
- Heping Huang
- Min Li
- Yang Liu
- Longxiang Tu
- Deming Liu
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Affiliations: Burns Institute, The First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, P.R. China, Department of Oncology, The Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, P.R. China, Medical College of Nanchang University, Nanchang, Jiangxi 330006, P.R. China
Published online on: October 23, 2017 https://doi.org/10.3892/ijmm.2017.3206
Pages: 87-94
Copyright: © Fu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Patient specific induced pluripotent stem cells (iPSCs) have been recognized as a possible source of cells for skin tissue engineering. They have the potential to greatly benefit patients with large areas of burned skin or skin defects. However, the integration virus-based reprogramming method is associated with a high risk of genetic mutation and mouse embryonic fibroblast feeder-cells may be a pollutant. In the present study, human skin fibroblasts (HSFs) were successfully harvested from patients with burns and patient-specific iPSCs were generated using a non-integration method with a feeder-free approach. The octamer-binding transcription factor 4 (OCT4), sex-determining region Y box 2 (SOX2) and NANOG transcription factors were delivered using Sendai virus vectors. iPSCs exhibited representative human embryonic stem cell-like morphology and proliferation characteristics. They also expressed pluripotent markers, including OCT4, NANOG, SOX2, TRA181, stage-specific embryonic antigen 4 and TRA-160, and exhibited a normal karyotype. Teratoma and embryoid body formation revealed that iPSCs were able to differentiate into cells of all three germ layers in vitro and in vivo. The results of the present study demonstrate that HSFs derived from patients with burns, may be reprogrammed into stem cells with pluripotency, which provides a basis for cell‑based skin tissue engineering in the future.

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