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AMPK activation serves a critical role in mitochondria quality control via modulating mitophagy in the heart under chronic hypoxia

  • Authors:
    • Huagang Zhang
    • Bo Liu
    • Tianbo Li
    • Yun Zhu
    • Guiping Luo
    • Yunhan Jiang
    • Fuqin Tang
    • Zhao Jian
    • Yingbin Xiao
  • View Affiliations / Copyright

    Affiliations: Department of Cardiovascular Surgery, Xinqiao Hospital, The Third Military Medical University, Chongqing 400037, P.R. China
    Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 69-76
    |
    Published online on: October 26, 2017
       https://doi.org/10.3892/ijmm.2017.3213
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Abstract

Mitochondrial biogenesis is one of the generally accepted regulatory mechanisms in the heart under chronic hypoxia. The precise quantity and quality control of mitochondria is critical for the survival and function of cardiomyocytes. Mitochondrial autophagy, also known as mitophagy, which selectively eliminates dysfunctional and unwanted mitochondria, is the most important type of mitochondrial quality control. However, the detailed molecular mechanisms of mitophagy in cardiomyocytes have been largely undefined. The present study investigated the role of adenosine 5'‑monophosphate‑activated protein kinase (AMPK) in mitophagy regulation in cardiomyocytes under chronic hypoxia. H9c2 cells were cultured under hypoxic conditions (1% O2) for different time periods. Mitochondrial biogenesis was confirmed and hypoxia was found to induce the collapse of mitochondrial membrane potential (ΛΨm) and increase the number of dysfunctional mitochondria. As expected, mitochondrial autophagy was increased significantly in cardiomyocytes exposed to hypoxic conditions for 48 h. AMPK was activated under hypoxia. Notably, when the activation of AMPK was enhanced by the AMPK agonist AICAR, mitochondrial autophagy was increased accordingly. By contrast, when AMPK activation was blocked, mitochondrial autophagy was decreased and cardiomyocyte apoptosis was increased. In conclusion, in the present study, mitophagy was activated and played a crucial role in cardioprotection under chronic hypoxia. AMPK was involved in mitophagy regulation, thereby providing a potential therapeutic target for heart diseases associated with chronic hypoxia.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang H, Liu B, Li T, Zhu Y, Luo G, Jiang Y, Tang F, Jian Z and Xiao Y: AMPK activation serves a critical role in mitochondria quality control via modulating mitophagy in the heart under chronic hypoxia. Int J Mol Med 41: 69-76, 2018.
APA
Zhang, H., Liu, B., Li, T., Zhu, Y., Luo, G., Jiang, Y. ... Xiao, Y. (2018). AMPK activation serves a critical role in mitochondria quality control via modulating mitophagy in the heart under chronic hypoxia. International Journal of Molecular Medicine, 41, 69-76. https://doi.org/10.3892/ijmm.2017.3213
MLA
Zhang, H., Liu, B., Li, T., Zhu, Y., Luo, G., Jiang, Y., Tang, F., Jian, Z., Xiao, Y."AMPK activation serves a critical role in mitochondria quality control via modulating mitophagy in the heart under chronic hypoxia". International Journal of Molecular Medicine 41.1 (2018): 69-76.
Chicago
Zhang, H., Liu, B., Li, T., Zhu, Y., Luo, G., Jiang, Y., Tang, F., Jian, Z., Xiao, Y."AMPK activation serves a critical role in mitochondria quality control via modulating mitophagy in the heart under chronic hypoxia". International Journal of Molecular Medicine 41, no. 1 (2018): 69-76. https://doi.org/10.3892/ijmm.2017.3213
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang H, Liu B, Li T, Zhu Y, Luo G, Jiang Y, Tang F, Jian Z and Xiao Y: AMPK activation serves a critical role in mitochondria quality control via modulating mitophagy in the heart under chronic hypoxia. Int J Mol Med 41: 69-76, 2018.
APA
Zhang, H., Liu, B., Li, T., Zhu, Y., Luo, G., Jiang, Y. ... Xiao, Y. (2018). AMPK activation serves a critical role in mitochondria quality control via modulating mitophagy in the heart under chronic hypoxia. International Journal of Molecular Medicine, 41, 69-76. https://doi.org/10.3892/ijmm.2017.3213
MLA
Zhang, H., Liu, B., Li, T., Zhu, Y., Luo, G., Jiang, Y., Tang, F., Jian, Z., Xiao, Y."AMPK activation serves a critical role in mitochondria quality control via modulating mitophagy in the heart under chronic hypoxia". International Journal of Molecular Medicine 41.1 (2018): 69-76.
Chicago
Zhang, H., Liu, B., Li, T., Zhu, Y., Luo, G., Jiang, Y., Tang, F., Jian, Z., Xiao, Y."AMPK activation serves a critical role in mitochondria quality control via modulating mitophagy in the heart under chronic hypoxia". International Journal of Molecular Medicine 41, no. 1 (2018): 69-76. https://doi.org/10.3892/ijmm.2017.3213
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