Anti-inflammatory effects of a methanolic extract of Castanea seguinii Dode in LPS-induced RAW264.7 macrophage cells

Lim,Yourim; Park,Ji-Won; Kwon,Ok-Kyoung; Lee,Jae-Won; Lee,Han-Sol; Lee,Sangwoo; Choi,Sangho; Li,Wanyi; Jin,Hang; Han,Sang-Bae; Ahn,Kyung-Seop

doi:10.3892/ijmm.2017.3223

Anti-inflammatory effects of a methanolic extract of Castanea seguinii Dode in LPS-induced RAW264.7 macrophage cells

Authors:
- Yourim Lim
- Ji-Won Park
- Ok-Kyoung Kwon
- Jae-Won Lee
- Han-Sol Lee
- Sangwoo Lee
- Sangho Choi
- Wanyi Li
- Hang Jin
- Sang-Bae Han
- Kyung-Seop Ahn
View Affiliations

Affiliations: Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology, Cheonju-si, Chungcheongbuk-do 28116, Republic of Korea, International Biological Material Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 34141, Republic of Korea, Institute of Medicinal Plants, Yunnan Academy of Agricultural Sciences, Kunming, Yunnan 650200, P.R. China, College of Pharmacy, Chungbuk National University, Cheongju-si, Chungcheongbuk-do 28160, Republic of Korea
Published online on: October 27, 2017 https://doi.org/10.3892/ijmm.2017.3223
Pages: 391-398

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

Castanea extracts are known to have antioxidant properties and are used as a traditional medicine in China and Asia. However, the biological activity of Castanea seguinii Dode has remained to be fully elucidated. The present study investigated the anti-inflammatory effects of a Castanea seguinii Dode methanolic extract (CSME) on lipopolysaccharide-induced RAW264.7 macrophage cells. CSME inhibited the production of nitric oxide (NO) and the expression of inducible NO synthase. It also suppressed the production of the pro-inflammatory cytokines inteleukin-6 and tumor necrosis factor-α, as well as chemokine monocyte chemoattractant protein 1. In addition, CSME inhibited nuclear factor-κB (NF-κB) and mitogen-activated protein kinase (MAPK) signaling, while also downregulating transcription factor activator protein-1. Furthermore, CSME increased heme oxygenase 1 through the upregulation of NF (erythroid-derived 2)-like-2 (Nrf-2), which directly or indirectly affects inflammation. It also increased the phosphorylation of 5'-adenosine monophosphate-activated protein kinase (AMPK). In conclusion, CSME was demonstrated to exert its anti-inflammatory activities through the inhibition of the NF-κB and the MAPK signaling pathways, as well as the activation of Nrf-2 and AMPK. These results indicated that CSME may be a promising for development as a commercial anti-inflammatory medicine.

View Figures

View References

1	Gordon S: Alternative activation of macrophages. Nat Rev Immunol. 3:23–35. 2003. View Article : Google Scholar : PubMed/NCBI
2	Beutler B: Inferences, questions and possibilities in Toll-like receptor signalling. Nature. 430:257–263. 2004. View Article : Google Scholar : PubMed/NCBI
3	Yamamoto M, Sato S, Hemmi H, Uematsu S, Hoshino K, Kaisho T, Takeuchi O, Takeda K and Akira S: TRAM is specifically involved in the Toll-like receptor 4-mediated MyD88-independent signaling pathway. Nat Immunol. 4:1144–1150. 2003. View Article : Google Scholar : PubMed/NCBI
4	Hong JM, Kwon OK, Shin IS, Jeon CM, Shin NR, Lee J, Park SH, Bach TT, Hai V, Oh SR, et al: Anti-inflammatory effects of methanol extract of Canarium lyi C.D. Dai & Yakovlev in RAW 264.7 macrophages and a murine model of lipopolysaccharide-induced lung injury. Int J Mol Med. 35:1403–1410. 2015. View Article : Google Scholar : PubMed/NCBI
5	Islam T, Breton C, Salam MT, McConnell R, Wenten M, Gauderman WJ, Conti D, Van Den Berg D, Peters JM and Gilliland FD: Role of inducible nitric oxide synthase in asthma risk and lung function growth during adolescence. Thorax. 65:139–145. 2010. View Article : Google Scholar
6	Lu Y, Suh SJ, Kwak CH, Kwon KM, Seo CS, Li Y, Jin Y, Li X, Hwang SL, Kwon O, et al: Saucerneol F, a new lignan, inhibits iNOS expression via MAPKs, NF-κB and AP-1 inactivation in LPS-induced RAW264.7 cells. Int Immunopharmacol. 12:175–181. 2012. View Article : Google Scholar
7	Kim J, Cha YN and Surh YJ: A protective role of nuclear factor-erythroid 2-related factor-2 (Nrf2) in inflammatory disorders. Mutat Res. 690:12–23. 2010. View Article : Google Scholar
8	Kobayashi EH, Suzuki T, Funayama R, Nagashima T, Hayashi M, Sekine H, Tanaka N, Moriguchi T, Motohashi H, Nakayama K, et al: Nrf2 suppresses macrophage inflammatory response by blocking proinflammatory cytokine transcription. Nat Commun. 7:116242016. View Article : Google Scholar : PubMed/NCBI
9	Cai ZY, Sheng ZX and Yao H: Pachymic acid ameliorates sepsis-induced acute kidney injury by suppressing inflammation and activating the Nrf2/HO-1 pathway in rats. Eur Rev Med Pharmacol Sci. 21:1924–1931. 2017.PubMed/NCBI
10	Lee SH, Sohn DH, Jin XY, Kim SW, Choi SC and Seo GS: 2′,4′,6′-tris(methoxymethoxy) chalcone protects against trinitrobenzene sulfonic acid-induced colitis and blocks tumor necrosis factor-alpha-induced intestinal epithelial inflammation via heme oxygenase 1-dependent and independent pathways. Biochem Pharmacol. 74:870–880. 2007. View Article : Google Scholar : PubMed/NCBI
11	Salminen A, Hyttinen JM and Kaarniranta K: AMP-activated protein kinase inhibits NF-κB signaling and inflammation: Impact on healthspan and lifespan. J Mol Med (Berl). 89:667–676. 2011. View Article : Google Scholar
12	Mo C, Wang L, Zhang J, Numazawa S, Tang H, Tang X, Han X, Li J, Yang M, Wang Z, et al: The crosstalk between Nrf2 and AMPK signal pathways is important for the anti-inflammatory effect of berberine in LPS-stimulated macrophages and endotoxin-shocked mice. Antioxid Redox Signal. 20:574–588. 2014. View Article : Google Scholar :
13	Kolayli S, Can Z, Yildiz O, Sahin H and Karaoglu SA: A comparative study of the antihyaluronidase, antiurease, antioxidant, antimicrobial and physicochemical properties of different unifloral degrees of chestnut (Castanea sativa Mill.) honeys. J Enzyme Inhib Med Chem. 31(Suppl 3): 96–104. 2016. View Article : Google Scholar : PubMed/NCBI
14	Zlatanov MD, Antova GA, Angelova-Romova MJ and Teneva OT: Lipid composition of Castanea sativa Mill. and Aesculus hippocastanum fruit oils. J Sci Food Agric. 93:661–666. 2013. View Article : Google Scholar
15	Ahn K: The worldwide trend of using botanical drugs and strategies for developing global drugs. BMB Rep. 50:111–116. 2017. View Article : Google Scholar :
16	Chen IT, Hsu PH, Hsu WC, Chen NJ and Tseng PH: Polyubiquitination of transforming growth factor β-activated Kinase 1 (TAK1) at lysine 562 residue regulates TLR4-mediated JNK and p38 MAPK activation. Sci Rep. 5:123002015. View Article : Google Scholar
17	Kueanjinda P, Roytrakul S and Palaga T: A novel role of numb as a regulator of pro-inflammatory cytokine production in macrophages in response to Toll-like receptor 4. Sci Rep. 5:127842015. View Article : Google Scholar : PubMed/NCBI
18	Ajibade AA, Wang HY and Wang RF: Cell type-specific function of TAK1 in innate immune signaling. Trends Immunol. 34:307–316. 2013. View Article : Google Scholar : PubMed/NCBI
19	Park JW, Kwon OK, Yuniato P, Marwoto B, Lee J, Oh SR, Kim JH and Ahn KS: Amelioration of an LPS-induced inflammatory response using a methanolic extract of Lagerstroemia ovalifolia to suppress the activation of NF-κB in RAW264.7 macrophages. Int J Mol Med. 38:482–490. 2016. View Article : Google Scholar : PubMed/NCBI
20	Arthur JS and Ley SC: Mitogen-activated protein kinases in innate immunity. Nat Rev Immunol. 13:679–692. 2013. View Article : Google Scholar : PubMed/NCBI
21	Chang L and Karin M: Mammalian MAP kinase signalling cascades. Nature. 410:37–40. 2001. View Article : Google Scholar : PubMed/NCBI
22	Nateri AS, Spencer-Dene B and Behrens A: Interaction of phosphorylated c-Jun with TCF4 regulates intestinal cancer development. Nature. 437:281–285. 2005. View Article : Google Scholar : PubMed/NCBI
23	Whitmarsh AJ and Davis RJ: Transcription factor AP-1 regulation by mitogen-activated protein kinase signal transduction pathways. J Mol Med (Berl). 74:589–607. 1996. View Article : Google Scholar
24	Guo RF and Ward PA: Role of oxidants in lung injury during sepsis. Antioxid Redox Signal. 9:1991–2002. 2007. View Article : Google Scholar : PubMed/NCBI
25	Tamion F, Richard V, Renet S and Thuillez C: Intestinal preconditioning prevents inflammatory response by modulating heme oxygenase-1 expression in endotoxic shock model. Am J Physiol Gastrointest Liver Physiol. 293:G1308–G1314. 2007. View Article : Google Scholar : PubMed/NCBI
26	Sag D, Carling D, Stout RD and Suttles J: Adenosine 5′-mono-phosphate-activated protein kinase promotes macrophage polarization to an anti-inflammatory functional phenotype. J Immunol. 181:8633–8641. 2008. View Article : Google Scholar : PubMed/NCBI
27	Salminen A and Kaarniranta K: AMP-activated protein kinase (AMPK) controls the aging process via an integrated signaling network. Ageing Res Rev. 11:230–241. 2012. View Article : Google Scholar
28	Liu X, Wang N, Fan S, Zheng X, Yang Y, Zhu Y, Lu Y, Chen Q, Zhou H and Zheng J: The citrus flavonoid naringenin confers protection in a murine endotoxaemia model through AMPK-ATF3-dependent negative regulation of the TLR4 signalling pathway. Sci Rep. 6:397352016. View Article : Google Scholar : PubMed/NCBI
29	Xue B, Yang Z, Wang X and Shi H: Omega-3 polyunsaturated fatty acids antagonize macrophage inflammation via activation of AMPK/SIRT1 pathway. PLoS One. 7:e459902012. View Article : Google Scholar : PubMed/NCBI