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International Journal of Molecular Medicine
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Article

Anti-inflammatory effects of a methanolic extract of Castanea seguinii Dode in LPS-induced RAW264.7 macrophage cells

  • Authors:
    • Yourim Lim
    • Ji-Won Park
    • Ok-Kyoung Kwon
    • Jae-Won Lee
    • Han-Sol Lee
    • Sangwoo Lee
    • Sangho Choi
    • Wanyi Li
    • Hang Jin
    • Sang-Bae Han
    • Kyung-Seop Ahn
  • View Affiliations / Copyright

    Affiliations: Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology, Cheonju-si, Chungcheongbuk-do 28116, Republic of Korea, International Biological Material Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 34141, Republic of Korea, Institute of Medicinal Plants, Yunnan Academy of Agricultural Sciences, Kunming, Yunnan 650200, P.R. China, College of Pharmacy, Chungbuk National University, Cheongju-si, Chungcheongbuk-do 28160, Republic of Korea
  • Pages: 391-398
    |
    Published online on: October 27, 2017
       https://doi.org/10.3892/ijmm.2017.3223
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Abstract

Castanea extracts are known to have antioxidant properties and are used as a traditional medicine in China and Asia. However, the biological activity of Castanea seguinii Dode has remained to be fully elucidated. The present study investigated the anti-inflammatory effects of a Castanea seguinii Dode methanolic extract (CSME) on lipopolysaccharide-induced RAW264.7 macrophage cells. CSME inhibited the production of nitric oxide (NO) and the expression of inducible NO synthase. It also suppressed the production of the pro-inflammatory cytokines inteleukin-6 and tumor necrosis factor-α, as well as chemokine monocyte chemoattractant protein 1. In addition, CSME inhibited nuclear factor-κB (NF-κB) and mitogen-activated protein kinase (MAPK) signaling, while also downregulating transcription factor activator protein-1. Furthermore, CSME increased heme oxygenase 1 through the upregulation of NF (erythroid-derived 2)-like-2 (Nrf-2), which directly or indirectly affects inflammation. It also increased the phosphorylation of 5'-adenosine monophosphate-activated protein kinase (AMPK). In conclusion, CSME was demonstrated to exert its anti-inflammatory activities through the inhibition of the NF-κB and the MAPK signaling pathways, as well as the activation of Nrf-2 and AMPK. These results indicated that CSME may be a promising for development as a commercial anti-inflammatory medicine.
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Copy and paste a formatted citation
Spandidos Publications style
Lim Y, Park J, Kwon O, Lee J, Lee H, Lee S, Choi S, Li W, Jin H, Han S, Han S, et al: Anti-inflammatory effects of a methanolic extract of Castanea seguinii Dode in LPS-induced RAW264.7 macrophage cells. Int J Mol Med 41: 391-398, 2018.
APA
Lim, Y., Park, J., Kwon, O., Lee, J., Lee, H., Lee, S. ... Ahn, K. (2018). Anti-inflammatory effects of a methanolic extract of Castanea seguinii Dode in LPS-induced RAW264.7 macrophage cells. International Journal of Molecular Medicine, 41, 391-398. https://doi.org/10.3892/ijmm.2017.3223
MLA
Lim, Y., Park, J., Kwon, O., Lee, J., Lee, H., Lee, S., Choi, S., Li, W., Jin, H., Han, S., Ahn, K."Anti-inflammatory effects of a methanolic extract of Castanea seguinii Dode in LPS-induced RAW264.7 macrophage cells". International Journal of Molecular Medicine 41.1 (2018): 391-398.
Chicago
Lim, Y., Park, J., Kwon, O., Lee, J., Lee, H., Lee, S., Choi, S., Li, W., Jin, H., Han, S., Ahn, K."Anti-inflammatory effects of a methanolic extract of Castanea seguinii Dode in LPS-induced RAW264.7 macrophage cells". International Journal of Molecular Medicine 41, no. 1 (2018): 391-398. https://doi.org/10.3892/ijmm.2017.3223
Copy and paste a formatted citation
x
Spandidos Publications style
Lim Y, Park J, Kwon O, Lee J, Lee H, Lee S, Choi S, Li W, Jin H, Han S, Han S, et al: Anti-inflammatory effects of a methanolic extract of Castanea seguinii Dode in LPS-induced RAW264.7 macrophage cells. Int J Mol Med 41: 391-398, 2018.
APA
Lim, Y., Park, J., Kwon, O., Lee, J., Lee, H., Lee, S. ... Ahn, K. (2018). Anti-inflammatory effects of a methanolic extract of Castanea seguinii Dode in LPS-induced RAW264.7 macrophage cells. International Journal of Molecular Medicine, 41, 391-398. https://doi.org/10.3892/ijmm.2017.3223
MLA
Lim, Y., Park, J., Kwon, O., Lee, J., Lee, H., Lee, S., Choi, S., Li, W., Jin, H., Han, S., Ahn, K."Anti-inflammatory effects of a methanolic extract of Castanea seguinii Dode in LPS-induced RAW264.7 macrophage cells". International Journal of Molecular Medicine 41.1 (2018): 391-398.
Chicago
Lim, Y., Park, J., Kwon, O., Lee, J., Lee, H., Lee, S., Choi, S., Li, W., Jin, H., Han, S., Ahn, K."Anti-inflammatory effects of a methanolic extract of Castanea seguinii Dode in LPS-induced RAW264.7 macrophage cells". International Journal of Molecular Medicine 41, no. 1 (2018): 391-398. https://doi.org/10.3892/ijmm.2017.3223
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