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Transforming growth factor β1 promotes migration and invasion in HepG2 cells: Epithelial‑to‑mesenchymal transition via JAK/STAT3 signaling

  • Authors:
    • Xiao‑Long Lin
    • Mihua Liu
    • Yuanbo Liu
    • Huijun Hu
    • Yongquan  Pan
    • Weiwen Zou
    • Xiaojuan Fan
    • Xuemei Hu
  • View Affiliations / Copyright

    Affiliations: Department of Pathology, Huizhou Third People's Hospital, Guangzhou Medical University, Huizhou, Guangdong 516002, P.R. China, Department of Clinical Laboratory, Affiliated Nanhua Hospital, University of South China, Hengyang, Hunan 421001, P.R. China, Department of Neurology, The Sixth People's Hospital of Huizhou, Huizhou, Guangdong 516211, P.R. China
    Copyright: © Lin et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 129-136
    |
    Published online on: November 1, 2017
       https://doi.org/10.3892/ijmm.2017.3228
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Abstract

Transforming growth factor β1 (TGFβ1) is a cytokine with multiple functions. TGFβ1 significantly induces migration and invasion of liver cancer cells. However, the molecular mechanisms underlying this effect remain unclear. Epithelial‑to‑mesenchymal transition (EMT) is crucial for the development of invasion and metastasis in human cancers. The aim of the present study was to determine whether TGFβ1‑induced EMT promoted migration and invasion in HepG2 cells. The underlying mechanism and the effect of EMT on HepG2 cells were also investigated. The results demonstrated that TGFβ1 may induce EMT to promote migration and invasion of HepG2 cells, and this effect depends on activation of the Janus kinase/signal transducer and activator of transcription 3 (JAK/STAT3) signaling pathway. JAK/STAT3 signaling is involved in human malignancies, including lung cancer, and is implicated in cell transformation, tumorigenicity, EMT and metastasis. In the present study, TGFβ1 also activated JAK/STAT3 signaling in HepG2 cells and promoted Twist expression, but these events were abolished by treatment with the STAT3 inhibitor AG490. Additionally, Twist siRNA blocked TGFβ1‑induced EMT. Thus, TGFβ1 was shown to induce EMT, thereby promoting the migration and invasion of HepG2 cells via JAK/STAT3/Twist signaling.
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Copy and paste a formatted citation
Spandidos Publications style
Lin XL, Liu M, Liu Y, Hu H, Pan Y, Zou W, Fan X and Hu X: Transforming growth factor β1 promotes migration and invasion in HepG2 cells: Epithelial‑to‑mesenchymal transition via JAK/STAT3 signaling. Int J Mol Med 41: 129-136, 2018.
APA
Lin, X., Liu, M., Liu, Y., Hu, H., Pan, Y., Zou, W. ... Hu, X. (2018). Transforming growth factor β1 promotes migration and invasion in HepG2 cells: Epithelial‑to‑mesenchymal transition via JAK/STAT3 signaling. International Journal of Molecular Medicine, 41, 129-136. https://doi.org/10.3892/ijmm.2017.3228
MLA
Lin, X., Liu, M., Liu, Y., Hu, H., Pan, Y., Zou, W., Fan, X., Hu, X."Transforming growth factor β1 promotes migration and invasion in HepG2 cells: Epithelial‑to‑mesenchymal transition via JAK/STAT3 signaling". International Journal of Molecular Medicine 41.1 (2018): 129-136.
Chicago
Lin, X., Liu, M., Liu, Y., Hu, H., Pan, Y., Zou, W., Fan, X., Hu, X."Transforming growth factor β1 promotes migration and invasion in HepG2 cells: Epithelial‑to‑mesenchymal transition via JAK/STAT3 signaling". International Journal of Molecular Medicine 41, no. 1 (2018): 129-136. https://doi.org/10.3892/ijmm.2017.3228
Copy and paste a formatted citation
x
Spandidos Publications style
Lin XL, Liu M, Liu Y, Hu H, Pan Y, Zou W, Fan X and Hu X: Transforming growth factor β1 promotes migration and invasion in HepG2 cells: Epithelial‑to‑mesenchymal transition via JAK/STAT3 signaling. Int J Mol Med 41: 129-136, 2018.
APA
Lin, X., Liu, M., Liu, Y., Hu, H., Pan, Y., Zou, W. ... Hu, X. (2018). Transforming growth factor β1 promotes migration and invasion in HepG2 cells: Epithelial‑to‑mesenchymal transition via JAK/STAT3 signaling. International Journal of Molecular Medicine, 41, 129-136. https://doi.org/10.3892/ijmm.2017.3228
MLA
Lin, X., Liu, M., Liu, Y., Hu, H., Pan, Y., Zou, W., Fan, X., Hu, X."Transforming growth factor β1 promotes migration and invasion in HepG2 cells: Epithelial‑to‑mesenchymal transition via JAK/STAT3 signaling". International Journal of Molecular Medicine 41.1 (2018): 129-136.
Chicago
Lin, X., Liu, M., Liu, Y., Hu, H., Pan, Y., Zou, W., Fan, X., Hu, X."Transforming growth factor β1 promotes migration and invasion in HepG2 cells: Epithelial‑to‑mesenchymal transition via JAK/STAT3 signaling". International Journal of Molecular Medicine 41, no. 1 (2018): 129-136. https://doi.org/10.3892/ijmm.2017.3228
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