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Article Open Access

Broadleaf Mahonia attenuates granulomatous lobular mastitis‑associated inflammation by inhibiting CCL‑5 expression in macrophages

  • Authors:
    • Zhiyu Wang
    • Neng Wang
    • Xiaoyan Liu
    • Qi Wang
    • Biao Xu
    • Pengxi Liu
    • Huayu Zhu
    • Jianping Chen
    • Honglin Situ
    • Yi Lin
  • View Affiliations / Copyright

    Affiliations: Department of Mammary Disease, Guangdong Provincial Hospital of Chinese Medicine, Guangzhou, Guangdong 510006, P.R. China, Department of Breast Oncology, Sun Yat‑Sen Univeristy Cancer Center, State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Guangzhou, Guangdong Guangzhou, Guangdong 510060, P.R. China, Institute of Clinical Pharmacology, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong 510006, P.R. China, School of Chinese Medicine, The University of Hong Kong, Hong Kong, SAR 00852, P.R. China
    Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 340-352
    |
    Published online on: November 9, 2017
       https://doi.org/10.3892/ijmm.2017.3246
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Abstract

Granulomatous lobular mastitis (GLM) is a type of chronic mammary inflammation with unclear etiology. Currently systematic corticosteroids and methitrexate are considered as the main drugs for GLM treatment, but a high toxicity and risk of recurrence greatly limit their application. It is therefore an urgent requirement that safe and efficient natural drugs are found to improve the GLM prognosis. Broadleaf Mahonia (BM) is a traditional Chinese herb that is believed to have anti‑inflammatory properties according to ancient records of traditional Chinese medicine. The present study investigated this belief and demonstrated that BM significantly inhibited the expression of interleukin‑1β (IL‑1β), IL‑6, cyclooxygenase‑2 and inducible nitric oxide synthase in RAW264.7 cells, but had little influence on the cell viability, cell cycle and apoptosis. Meanwhile, the lipopolysaccharide‑induced elevation of reactive oxygen species and nitric oxide was also blocked following BM treatment, accompanied with decreased activity of nuclear factor‑κB and MAPK signaling. A cytokine array further validated that BM exhibited significant inhibitory effects on several chemoattractants, including chemokine (C‑C motif) ligand (CCL)‑2, CCL‑3, CCL‑5 and secreted tumor necrosis factor receptor 1, among which CCL‑5 exhibited the highest inhibition ratio in cell and clinical GLM specimens. Collectively, the results show that BM is a novel effective anti‑inflammatory herb in vitro and ex vivo, and that CCL‑5 may be closely associated with GLM pathogenesis.
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Copy and paste a formatted citation
Spandidos Publications style
Wang Z, Wang N, Liu X, Wang Q, Xu B, Liu P, Zhu H, Chen J, Situ H, Lin Y, Lin Y, et al: Broadleaf Mahonia attenuates granulomatous lobular mastitis‑associated inflammation by inhibiting CCL‑5 expression in macrophages. Int J Mol Med 41: 340-352, 2018.
APA
Wang, Z., Wang, N., Liu, X., Wang, Q., Xu, B., Liu, P. ... Lin, Y. (2018). Broadleaf Mahonia attenuates granulomatous lobular mastitis‑associated inflammation by inhibiting CCL‑5 expression in macrophages. International Journal of Molecular Medicine, 41, 340-352. https://doi.org/10.3892/ijmm.2017.3246
MLA
Wang, Z., Wang, N., Liu, X., Wang, Q., Xu, B., Liu, P., Zhu, H., Chen, J., Situ, H., Lin, Y."Broadleaf Mahonia attenuates granulomatous lobular mastitis‑associated inflammation by inhibiting CCL‑5 expression in macrophages". International Journal of Molecular Medicine 41.1 (2018): 340-352.
Chicago
Wang, Z., Wang, N., Liu, X., Wang, Q., Xu, B., Liu, P., Zhu, H., Chen, J., Situ, H., Lin, Y."Broadleaf Mahonia attenuates granulomatous lobular mastitis‑associated inflammation by inhibiting CCL‑5 expression in macrophages". International Journal of Molecular Medicine 41, no. 1 (2018): 340-352. https://doi.org/10.3892/ijmm.2017.3246
Copy and paste a formatted citation
x
Spandidos Publications style
Wang Z, Wang N, Liu X, Wang Q, Xu B, Liu P, Zhu H, Chen J, Situ H, Lin Y, Lin Y, et al: Broadleaf Mahonia attenuates granulomatous lobular mastitis‑associated inflammation by inhibiting CCL‑5 expression in macrophages. Int J Mol Med 41: 340-352, 2018.
APA
Wang, Z., Wang, N., Liu, X., Wang, Q., Xu, B., Liu, P. ... Lin, Y. (2018). Broadleaf Mahonia attenuates granulomatous lobular mastitis‑associated inflammation by inhibiting CCL‑5 expression in macrophages. International Journal of Molecular Medicine, 41, 340-352. https://doi.org/10.3892/ijmm.2017.3246
MLA
Wang, Z., Wang, N., Liu, X., Wang, Q., Xu, B., Liu, P., Zhu, H., Chen, J., Situ, H., Lin, Y."Broadleaf Mahonia attenuates granulomatous lobular mastitis‑associated inflammation by inhibiting CCL‑5 expression in macrophages". International Journal of Molecular Medicine 41.1 (2018): 340-352.
Chicago
Wang, Z., Wang, N., Liu, X., Wang, Q., Xu, B., Liu, P., Zhu, H., Chen, J., Situ, H., Lin, Y."Broadleaf Mahonia attenuates granulomatous lobular mastitis‑associated inflammation by inhibiting CCL‑5 expression in macrophages". International Journal of Molecular Medicine 41, no. 1 (2018): 340-352. https://doi.org/10.3892/ijmm.2017.3246
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