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Clinical significance of interleukin‑6 and inducible nitric oxide synthase in ketamine‑induced cystitis

  • Authors:
    • Chi‑Jung Huang
    • Fa‑Kung Lee
    • Shao‑Kuan Chen
    • Chih‑Cheng Chien
    • Sheng‑Tang Wu
    • Yen‑Chieh Wang
  • View Affiliations / Copyright

    Affiliations: Department of Medical Research, Cathay General Hospital, Taipei 10630, Taiwan, R.O.C., School of Medicine, College of Medicine, Fu Jen Catholic University, New Taipei 24205, Taiwan, R.O.C., Division of Urology, Department of Surgery, Tri‑Service General Hospital, National Defense Medical Center, Taipei 11490, Taiwan, R.O.C.
    Copyright: © Huang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 836-844
    |
    Published online on: November 17, 2017
       https://doi.org/10.3892/ijmm.2017.3264
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Abstract

Ketamine is an ionotropic glutamatergic N‑methyl‑D‑aspartate receptor antagonist, which is widely used among recreational drug abusers. Ketamine abusers exhibit substantially reduced bladder capacity, which can lead to urinary frequency. The molecular pathogenesis of ketamine‑induced cystitis has been scarcely reported. Given previous clinical findings, it may be hypothesized that pathological alterations in smooth muscle cells (SMCs) of the urinary bladder serve a crucial role in the mechanism underlying cystitis. In the present study, two lineages of SMCs, one from differentiated foreskin‑derived fibroblast‑like stromal cells and the other from cultured normal aortic SMCs, were used to study ketamine‑induced molecular alterations. Polymerase chain reaction was used to study the effects of ketamine on oxidative stress. The effects of adjuvant chemotherapy with cyclophosphamide (CTX) were also investigated. The results indicated that the expression levels of interleukin‑6 and inducible nitric oxide synthase (iNOS) were decreased, whereas collagen expression and deposition were increased in ketamine‑treated SMCs. Conversely, treatment with CTX restored the expression of iNOS, which may prevent or limit oxidative damage. In conclusion, the present study demonstrated that ketamine may induce several molecular alterations in SMCs and these changes may be associated with the clinical symptoms observed in ketamine abusers. In addition, the specific chemotherapeutic agent CTX may reverse these ketamine‑induced aberrations.
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Copy and paste a formatted citation
Spandidos Publications style
Huang CJ, Lee FK, Chen SK, Chien CC, Wu ST and Wang YC: Clinical significance of interleukin‑6 and inducible nitric oxide synthase in ketamine‑induced cystitis. Int J Mol Med 41: 836-844, 2018.
APA
Huang, C., Lee, F., Chen, S., Chien, C., Wu, S., & Wang, Y. (2018). Clinical significance of interleukin‑6 and inducible nitric oxide synthase in ketamine‑induced cystitis. International Journal of Molecular Medicine, 41, 836-844. https://doi.org/10.3892/ijmm.2017.3264
MLA
Huang, C., Lee, F., Chen, S., Chien, C., Wu, S., Wang, Y."Clinical significance of interleukin‑6 and inducible nitric oxide synthase in ketamine‑induced cystitis". International Journal of Molecular Medicine 41.2 (2018): 836-844.
Chicago
Huang, C., Lee, F., Chen, S., Chien, C., Wu, S., Wang, Y."Clinical significance of interleukin‑6 and inducible nitric oxide synthase in ketamine‑induced cystitis". International Journal of Molecular Medicine 41, no. 2 (2018): 836-844. https://doi.org/10.3892/ijmm.2017.3264
Copy and paste a formatted citation
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Spandidos Publications style
Huang CJ, Lee FK, Chen SK, Chien CC, Wu ST and Wang YC: Clinical significance of interleukin‑6 and inducible nitric oxide synthase in ketamine‑induced cystitis. Int J Mol Med 41: 836-844, 2018.
APA
Huang, C., Lee, F., Chen, S., Chien, C., Wu, S., & Wang, Y. (2018). Clinical significance of interleukin‑6 and inducible nitric oxide synthase in ketamine‑induced cystitis. International Journal of Molecular Medicine, 41, 836-844. https://doi.org/10.3892/ijmm.2017.3264
MLA
Huang, C., Lee, F., Chen, S., Chien, C., Wu, S., Wang, Y."Clinical significance of interleukin‑6 and inducible nitric oxide synthase in ketamine‑induced cystitis". International Journal of Molecular Medicine 41.2 (2018): 836-844.
Chicago
Huang, C., Lee, F., Chen, S., Chien, C., Wu, S., Wang, Y."Clinical significance of interleukin‑6 and inducible nitric oxide synthase in ketamine‑induced cystitis". International Journal of Molecular Medicine 41, no. 2 (2018): 836-844. https://doi.org/10.3892/ijmm.2017.3264
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