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Article

Role of the c-Jun N-terminal kinase signaling pathway in the activation of trypsinogen in rat pancreatic acinar cells

  • Authors:
    • Zhengpeng Yang
    • Weiguang Yang
    • Ming Lu
    • Zhituo Li
    • Xin Qiao
    • Bei Sun
    • Weihui Zhang
    • Dongbo Xue
  • View Affiliations / Copyright

    Affiliations: Department of General Surgery, The First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150001, P.R. China, Department of Surgery, David Geffen School of Medicine, University of Califonia at Los Angeles, Los Angeles, CA 90095, USA
  • Pages: 1119-1126
    |
    Published online on: November 17, 2017
       https://doi.org/10.3892/ijmm.2017.3266
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Abstract

Bile acid causes trypsinogen activation in pancreatic acinar cells through a complex process. Additional research is required to further elucidate which signaling pathways affect trypsinogen activation when activated. the changes in the whole‑genome expression profile of AR42J cells under the effect of taurolithocholic acid 3‑sulfate (TLC‑S) were investigated. Furthermore, gene groups that may play a regulatory role were analyzed using the modular approach of biological networks. The aim of the present study was to improve our understanding of the changes in TLC‑S‑stimulated AR42J cells through a genetic functional modular analysis. whole‑genome expression profile chip arrays were applied to detect genes that were differentially expressed in pancreatic acinar AR42J cells treated with TLC‑S for 20 min. Based on the human protein reference database, a protein‑protein interaction network was obtained, which was then processed by CFinder software to derive 14 modules. Among these 14 modules, the gene ontology biological processes enrichment analysis identified two as modules of interest. Kyoto encyclopedia of genes and genomes map analysis revealed that MAP2K4, MAPK8 and FLNA are part of the c-Jun N-terminal kinase (JNK) pathway. The JNK signaling pathway is involved in regulating trypsinogen activation in rat pancreatic AR42J cells. Next, a regulatory network of seven kinase inhibitors was constructed. SP600125 is an ATP‑competitive, efficient, selective and reversible inhibitor of JNK. the results were verified by four sets of experiments and demonstrated that trypsinogen activation is mediated by the JNK signaling pathway in the pathogenesis of acute pancreatitis (AP). The present study provided a useful reference for better understanding the pathogenesis of AP and identifying new targets to regulate trypsinogen activation, in addition to providing valuable information for the treatment of AP.
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Copy and paste a formatted citation
Spandidos Publications style
Yang Z, Yang W, Lu M, Li Z, Qiao X, Sun B, Zhang W and Xue D: Role of the c-Jun N-terminal kinase signaling pathway in the activation of trypsinogen in rat pancreatic acinar cells. Int J Mol Med 41: 1119-1126, 2018.
APA
Yang, Z., Yang, W., Lu, M., Li, Z., Qiao, X., Sun, B. ... Xue, D. (2018). Role of the c-Jun N-terminal kinase signaling pathway in the activation of trypsinogen in rat pancreatic acinar cells. International Journal of Molecular Medicine, 41, 1119-1126. https://doi.org/10.3892/ijmm.2017.3266
MLA
Yang, Z., Yang, W., Lu, M., Li, Z., Qiao, X., Sun, B., Zhang, W., Xue, D."Role of the c-Jun N-terminal kinase signaling pathway in the activation of trypsinogen in rat pancreatic acinar cells". International Journal of Molecular Medicine 41.2 (2018): 1119-1126.
Chicago
Yang, Z., Yang, W., Lu, M., Li, Z., Qiao, X., Sun, B., Zhang, W., Xue, D."Role of the c-Jun N-terminal kinase signaling pathway in the activation of trypsinogen in rat pancreatic acinar cells". International Journal of Molecular Medicine 41, no. 2 (2018): 1119-1126. https://doi.org/10.3892/ijmm.2017.3266
Copy and paste a formatted citation
x
Spandidos Publications style
Yang Z, Yang W, Lu M, Li Z, Qiao X, Sun B, Zhang W and Xue D: Role of the c-Jun N-terminal kinase signaling pathway in the activation of trypsinogen in rat pancreatic acinar cells. Int J Mol Med 41: 1119-1126, 2018.
APA
Yang, Z., Yang, W., Lu, M., Li, Z., Qiao, X., Sun, B. ... Xue, D. (2018). Role of the c-Jun N-terminal kinase signaling pathway in the activation of trypsinogen in rat pancreatic acinar cells. International Journal of Molecular Medicine, 41, 1119-1126. https://doi.org/10.3892/ijmm.2017.3266
MLA
Yang, Z., Yang, W., Lu, M., Li, Z., Qiao, X., Sun, B., Zhang, W., Xue, D."Role of the c-Jun N-terminal kinase signaling pathway in the activation of trypsinogen in rat pancreatic acinar cells". International Journal of Molecular Medicine 41.2 (2018): 1119-1126.
Chicago
Yang, Z., Yang, W., Lu, M., Li, Z., Qiao, X., Sun, B., Zhang, W., Xue, D."Role of the c-Jun N-terminal kinase signaling pathway in the activation of trypsinogen in rat pancreatic acinar cells". International Journal of Molecular Medicine 41, no. 2 (2018): 1119-1126. https://doi.org/10.3892/ijmm.2017.3266
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