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Article

Stromal cell‑derived factor‑1 induces matrix metalloproteinase expression in human endplate chondrocytes, cartilage endplate degradation in explant culture, and the amelioration of nucleus pulposus degeneration in vivo

  • Authors:
    • Hua Zhang
    • Ting Zhu
    • Li Zhang
    • Qionghua Wu
  • View Affiliations / Copyright

    Affiliations: Department of Orthopaedic Surgery, The Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang 310009, P.R. China, Department of Clinical Laboratory, Hangzhou Red Cross Hospital, Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310000, P.R. China
  • Pages: 969-976
    |
    Published online on: November 22, 2017
       https://doi.org/10.3892/ijmm.2017.3278
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Abstract

Intervertebral disc (IVD) degeneration is a strong etiological factor in chronic lower back pain. Stem cell migration toward the site of IVD degeneration for regeneration is restricted by avascularity and distance. Our previous study indicated that the expression of stromal cell‑derived factor‑1 (SDF‑1) and its receptor, C-X-C chemokine receptor type 4 (CXCR4) was upregulated in degenerated cartilage endplate (CEP) and nucleus pulposus (NP). In the present study, SDF‑1 increased CXCR4 mRNA and protein expression in human endplate chondrocytes in a dose‑dependent manner. The results of reverse transcription-quantitative polymerase chain reaction, western blotting and zymography indicated that SDF‑1 increased matrix metalloproteinase (MMP)‑1, ‑3 and ‑13 mRNA and protein expression in human endplate chondrocytes in a dose‑dependent manner. The results of zymography suggested that SDF‑1 also increased MMP‑2 and ‑9 protein expression in a dose‑dependent manner. The CXCR4‑specific chemical inhibitor AMD3100 significantly decreased the levels of MMP‑1, ‑2, ‑3, ‑9 and ‑13 expression. In a human cartilage explant culture model, SDF‑1 accelerated the degradation of extracellular matrix (ECM), and AMD3100 decreased cartilage cleavage. However, in a rat tail disc degeneration model, the injection of SDF‑1 into the NP resulted in the retention of dense areas of proteoglycan matrix and enhanced NP regeneration. These results suggest that SDF‑1, as an inflammatory cytokine, induces MMP expression in human endplate chondrocytes and that ECM remodeling in the CEP may be a favorable factor of endogenous stem cell homing into the NP for regeneration in vivo.
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1 

Chan WC, Sze KL, Samartzis D, Leung VY and Chan D: Structure and biology of the intervertebral disk in health and disease. Orthop Clin North Am. 42:447–464. 2011. View Article : Google Scholar : PubMed/NCBI

2 

Li Z, Peroglio M, Alini M and Grad S: Potential and limitations of intervertebral disc endogenous repair. Curr Stem Cell Res Ther. 10:329–338. 2015. View Article : Google Scholar : PubMed/NCBI

3 

Roughley PJ: Biology of intervertebral disc aging and degeneration: Involvement of the extracellular matrix. Spine. 29:2691–2699. 2004. View Article : Google Scholar : PubMed/NCBI

4 

Goupille P, Jayson MI, Valat JP and Freemont AJ: Matrix metalloproteinases: The clue to intervertebral disc degeneration? Spine. 23:1612–1626. 1998. View Article : Google Scholar : PubMed/NCBI

5 

Pockert AJ, Richardson SM, Le Maitre CL, Lyon M, Deakin JA, Buttle DJ, Freemont AJ and Hoyland JA: Modified expression of the ADAMTS enzymes and tissue inhibitor of metalloproteinases 3 during human intervertebral disc degeneration. Arthritis Rheum. 60:482–491. 2009. View Article : Google Scholar : PubMed/NCBI

6 

Zhang H, Zhang L, Chen L, Li W, Li F and Chen Q: Stromal cell-derived factor-1 and its receptor CXCR4 are upregulated expression in degenerated intervertebral discs. Int J Med Sci. 11:240–245. 2014. View Article : Google Scholar : PubMed/NCBI

7 

Kanbe K, Takagishi K and Chen Q: Stimulation of matrix metalloprotease 3 release from human chondrocytes by the interaction of stromal cell-derived factor 1 and CXC chemokine receptor 4. Arthritis Rheum. 46:130–137. 2002. View Article : Google Scholar : PubMed/NCBI

8 

Chiu YC, Yang RS, Hsieh KH, Fong YC, Way TD, Lee TS, Wu HC, Fu WM and Tang CH: Stromal cell-derived factor-1 induces matrix metalloprotease-13 expression in human chondrocytes. Mol Pharmacol. 72:695–703. 2007. View Article : Google Scholar : PubMed/NCBI

9 

Kanbe K, Takemura T, Takeuchi K, Chen Q, Takagishi K and Inoue K: Synovectomy reduces stromal-cell-derived factor-1 (SDF-1) which is involved in the destruction of cartilage in osteoarthritis and rheumatoid arthritis. J Bone Joint Surg Br. 86:296–300. 2004. View Article : Google Scholar : PubMed/NCBI

10 

Seta N and Kuwana M: Human circulating monocytes as multi-potential progenitors. Keio J Med. 56:41–47. 2007. View Article : Google Scholar : PubMed/NCBI

11 

Krause DS: Plasticity of marrow-derived stem cells. Gene Ther. 9:754–758. 2002. View Article : Google Scholar : PubMed/NCBI

12 

Sakai D, Nishimura K, Tanaka M, Nakajima D, Grad S, Alini M, Kawada H, Ando K and Mochida J: Migration of bone marrow-derived cells for endogenous repair in a new tail-looping disc degeneration model in the mouse: A pilot study. Spine J. 15:1356–1365. 2015. View Article : Google Scholar

13 

Illien-Jünger S, Pattappa G, Peroglio M, Benneker LM, Stoddart MJ, Sakai D, Mochida J, Grad S and Alini M: Homing of mesenchymal stem cells in induced degenerative intervertebral discs in a whole organ culture system. Spine. 37:1865–1873. 2012. View Article : Google Scholar : PubMed/NCBI

14 

Pereira CL, Gonçalves RM, Peroglio M, Pattappa G, D'Este M, Eglin D, Barbosa MA, Alini M and Grad S: The effect of hyaluronan-based delivery of stromal cell-derived factor-1 on the recruitment of MSCs in degenerating intervertebral discs. Biomaterials. 35:8144–8153. 2014. View Article : Google Scholar : PubMed/NCBI

15 

Pfirrmann CW, Metzdorf A, Zanetti M, Hodler J and Boos N: Magnetic resonance classification of lumbar intervertebral disc degeneration. Spine. 26:1873–1878. 2001. View Article : Google Scholar : PubMed/NCBI

16 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta Delta C(T)) Method. Methods. 25:402–408. 2001. View Article : Google Scholar

17 

Chen P, Zhu S, Wang Y, Mu Q, Wu Y, Xia Q, Zhang X, Sun H, Tao J, Hu H, et al: The amelioration of cartilage degeneration by ADAMTS-5 inhibitor delivered in a hyaluronic acid hydrogel. Biomaterials. 35:2827–2836. 2014. View Article : Google Scholar : PubMed/NCBI

18 

Guide for the Care and Use of Laboratory Animals. (NIH Publication No. 85-23). Revised 1985.

19 

Mao HJ, Chen QX, Han B, Li FC, Feng J, Shi ZL, Lin M and Wang J: The effect of injection volume on disc degeneration in a rat tail model. Spine. 36:E1062–E1069. 2011. View Article : Google Scholar : PubMed/NCBI

20 

Wuelling M and Vortkamp A: Cartilage explant cultures. Methods Mol Biol. 1130:89–97. 2014. View Article : Google Scholar : PubMed/NCBI

21 

Minina E, Wenzel HM, Kreschel C, Karp S, Gaffield W, McMahon AP and Vortkamp A: BMP and Ihh/PTHrP signaling interact to coordinate chondrocyte proliferation and differentiation. Development. 128:4523–4534. 2001.PubMed/NCBI

22 

Song RH, Tortorella MD, Malfait AM, Alston JT, Yang Z, Arner EC and Griggs DW: Aggrecan degradation in human articular cartilage explants is mediated by both ADAMTS-4 and ADAMTS-5. Arthritis Rheum. 56:575–585. 2007. View Article : Google Scholar : PubMed/NCBI

23 

Grunewald M, Avraham I, Dor Y, Bachar-Lustig E, Itin A, Jung S, Chimenti S, Landsman L, Abramovitch R and Keshet E: VEGF-induced adult neovascularization: Recruitment, retention, and role of accessory cells. Cell. 124:175–189. 2006. View Article : Google Scholar : PubMed/NCBI

24 

Son BR, Marquez-Curtis LA, Kucia M, Wysoczynski M, Turner AR, Ratajczak J, Ratajczak MZ and Janowska-Wieczorek A: Migration of bone marrow and cord blood mesenchymal stem cells in vitro is regulated by stromal-derived factor-1-CXCR4 and hepatocyte growth factor-c-met axes and involves matrix metalloproteinases. Stem Cells. 24:1254–1264. 2006. View Article : Google Scholar : PubMed/NCBI

25 

Wynn RF, Hart CA, Corradi-Perini C, O'Neill L, Evans CA, Wraith JE, Fairbairn LJ and Bellantuono I: A small proportion of mesenchymal stem cells strongly expresses functionally active CXCR4 receptor capable of promoting migration to bone marrow. Blood. 104:2643–2645. 2004. View Article : Google Scholar : PubMed/NCBI

26 

Barkho BZ, Munoz AE, Li X, Li L, Cunningham LA and Zhao X: Endogenous matrix metalloproteinase (MMP)-3 and MMP-9 promote the differentiation and migration of adult neural progenitor cells in response to chemokines. Stem Cells. 26:3139–3149. 2008. View Article : Google Scholar : PubMed/NCBI

27 

Claes L, Recknagel S and Ignatius A: Fracture healing under healthy and inflammatory conditions. Nat Rev Rheumatol. 8:133–143. 2012. View Article : Google Scholar : PubMed/NCBI

28 

Mountziaris PM, Spicer PP, Kasper FK and Mikos AG: Harnessing and modulating inflammation in strategies for bone regeneration. Tissue Eng Part B Rev. 17:393–402. 2011. View Article : Google Scholar : PubMed/NCBI

29 

Boccafoschi F, Mosca C and Cannas M: Cardiovascular biomaterials: When the inflammatory response helps to efficiently restore tissue functionality? J Tissue Eng Regen Med. 8:253–267. 2014. View Article : Google Scholar

30 

Molinos M, Almeida CR, Caldeira J, Cunha C, Gonçalves RM and Barbosa MA: Inflammation in intervertebral disc degeneration and regeneration. J R Soc Interface. 12:201411912015. View Article : Google Scholar : PubMed/NCBI

31 

Zhang F, Leong W, Su K, Fang Y and Wang DA: A transduced living hyaline cartilage graft releasing transgenic stromal cell-derived factor-1 inducing endogenous stem cell homing in vivo. Tissue Eng Part A. 19:1091–1099. 2013. View Article : Google Scholar :

32 

Yu Y, Brouillette MJ, Seol D, Zheng H, Buckwalter JA and Martin JA: Functional full-thickness articular cartilage repair by rhSDF-1α loaded fibrin/HA hydrogel network via chondrogenic progenitor cells homing. Arthritis Rheumatol. 67:1274–1285. 2015. View Article : Google Scholar : PubMed/NCBI

33 

Chen P, Tao J, Zhu S, Cai Y, Mao Q, Yu D, Dai J and Ouyang H: Radially oriented collagen scaffold with SDF-1 promotes osteochondral repair by facilitating cell homing. Biomaterials. 39:114–123. 2015. View Article : Google Scholar

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Copy and paste a formatted citation
Spandidos Publications style
Zhang H, Zhu T, Zhang L and Wu Q: Stromal cell‑derived factor‑1 induces matrix metalloproteinase expression in human endplate chondrocytes, cartilage endplate degradation in explant culture, and the amelioration of nucleus pulposus degeneration in vivo. Int J Mol Med 41: 969-976, 2018.
APA
Zhang, H., Zhu, T., Zhang, L., & Wu, Q. (2018). Stromal cell‑derived factor‑1 induces matrix metalloproteinase expression in human endplate chondrocytes, cartilage endplate degradation in explant culture, and the amelioration of nucleus pulposus degeneration in vivo. International Journal of Molecular Medicine, 41, 969-976. https://doi.org/10.3892/ijmm.2017.3278
MLA
Zhang, H., Zhu, T., Zhang, L., Wu, Q."Stromal cell‑derived factor‑1 induces matrix metalloproteinase expression in human endplate chondrocytes, cartilage endplate degradation in explant culture, and the amelioration of nucleus pulposus degeneration in vivo". International Journal of Molecular Medicine 41.2 (2018): 969-976.
Chicago
Zhang, H., Zhu, T., Zhang, L., Wu, Q."Stromal cell‑derived factor‑1 induces matrix metalloproteinase expression in human endplate chondrocytes, cartilage endplate degradation in explant culture, and the amelioration of nucleus pulposus degeneration in vivo". International Journal of Molecular Medicine 41, no. 2 (2018): 969-976. https://doi.org/10.3892/ijmm.2017.3278
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang H, Zhu T, Zhang L and Wu Q: Stromal cell‑derived factor‑1 induces matrix metalloproteinase expression in human endplate chondrocytes, cartilage endplate degradation in explant culture, and the amelioration of nucleus pulposus degeneration in vivo. Int J Mol Med 41: 969-976, 2018.
APA
Zhang, H., Zhu, T., Zhang, L., & Wu, Q. (2018). Stromal cell‑derived factor‑1 induces matrix metalloproteinase expression in human endplate chondrocytes, cartilage endplate degradation in explant culture, and the amelioration of nucleus pulposus degeneration in vivo. International Journal of Molecular Medicine, 41, 969-976. https://doi.org/10.3892/ijmm.2017.3278
MLA
Zhang, H., Zhu, T., Zhang, L., Wu, Q."Stromal cell‑derived factor‑1 induces matrix metalloproteinase expression in human endplate chondrocytes, cartilage endplate degradation in explant culture, and the amelioration of nucleus pulposus degeneration in vivo". International Journal of Molecular Medicine 41.2 (2018): 969-976.
Chicago
Zhang, H., Zhu, T., Zhang, L., Wu, Q."Stromal cell‑derived factor‑1 induces matrix metalloproteinase expression in human endplate chondrocytes, cartilage endplate degradation in explant culture, and the amelioration of nucleus pulposus degeneration in vivo". International Journal of Molecular Medicine 41, no. 2 (2018): 969-976. https://doi.org/10.3892/ijmm.2017.3278
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