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Pentosan polysulfate ameliorates apoptosis and inflammation by suppressing activation of the p38 MAPK pathway in high glucose‑treated HK‑2 cells

  • Authors:
    • Ping Chen
    • Yang Yuan
    • Tianying Zhang
    • Bo Xu
    • Qing Gao
    • Tianjun Guan
  • View Affiliations / Copyright

    Affiliations: Department of Nephrology, Ningbo First Hospital, Ningbo, Zhejiang 315010, P.R. China, Department of Nephrology, Zhongshan Hospital, Xiamen University, Xiamen, Fujian 361004, P.R. China
    Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 908-914
    |
    Published online on: November 27, 2017
       https://doi.org/10.3892/ijmm.2017.3290
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Abstract

The apoptosis of tubular epithelial cells in diabetic nephropathy (DN) is commonly observed in human renal biopsies. Inflammation plays a key role in DN, and pentosan polysulfate (PPS) has been shown to largely attenuate the inflammation of nephropathy in aging diabetic mice. p38 mitogen‑activated protein kinase (p38 MAPK) plays a crucial role in tissue inflammation and cell apoptosis, and it is activated by hyperglycemia. In the present study, high glucose (HG)‑treated human renal proximal tubular epithelial cells (HK‑2) were used to examine the protective effects of PPS against HG‑stimulated apoptosis and inflammation. The results of the study revealed that PPS markedly suppressed the HG‑induced reduction in cell viability. Incubation of HK‑2 cells with HG activated the p38 MAPK pathway and, subsequently, as confirmed by western blot analysis and flow cytometry, increased cell apoptosis, which was blocked by PPS. In addition, PPS treatment significantly inhibited HG‑stimulated p38 MAPK and nuclear factor‑κB activation, and reduced the production of pro‑inflammatory cytokines, such as tumor necrosis factor‑α, interleukin (IL)‑1β and IL‑6. In conclusion, PPS ameliorates p38 MAPK‑mediated renal cell apoptosis and inflammation. The anti‑apoptotic actions and anti‑inflammatory effects of PPS prompt further investigation of this compound as a promising therapeutic agent against DN.
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Copy and paste a formatted citation
Spandidos Publications style
Chen P, Yuan Y, Zhang T, Xu B, Gao Q and Guan T: Pentosan polysulfate ameliorates apoptosis and inflammation by suppressing activation of the p38 MAPK pathway in high glucose‑treated HK‑2 cells. Int J Mol Med 41: 908-914, 2018.
APA
Chen, P., Yuan, Y., Zhang, T., Xu, B., Gao, Q., & Guan, T. (2018). Pentosan polysulfate ameliorates apoptosis and inflammation by suppressing activation of the p38 MAPK pathway in high glucose‑treated HK‑2 cells. International Journal of Molecular Medicine, 41, 908-914. https://doi.org/10.3892/ijmm.2017.3290
MLA
Chen, P., Yuan, Y., Zhang, T., Xu, B., Gao, Q., Guan, T."Pentosan polysulfate ameliorates apoptosis and inflammation by suppressing activation of the p38 MAPK pathway in high glucose‑treated HK‑2 cells". International Journal of Molecular Medicine 41.2 (2018): 908-914.
Chicago
Chen, P., Yuan, Y., Zhang, T., Xu, B., Gao, Q., Guan, T."Pentosan polysulfate ameliorates apoptosis and inflammation by suppressing activation of the p38 MAPK pathway in high glucose‑treated HK‑2 cells". International Journal of Molecular Medicine 41, no. 2 (2018): 908-914. https://doi.org/10.3892/ijmm.2017.3290
Copy and paste a formatted citation
x
Spandidos Publications style
Chen P, Yuan Y, Zhang T, Xu B, Gao Q and Guan T: Pentosan polysulfate ameliorates apoptosis and inflammation by suppressing activation of the p38 MAPK pathway in high glucose‑treated HK‑2 cells. Int J Mol Med 41: 908-914, 2018.
APA
Chen, P., Yuan, Y., Zhang, T., Xu, B., Gao, Q., & Guan, T. (2018). Pentosan polysulfate ameliorates apoptosis and inflammation by suppressing activation of the p38 MAPK pathway in high glucose‑treated HK‑2 cells. International Journal of Molecular Medicine, 41, 908-914. https://doi.org/10.3892/ijmm.2017.3290
MLA
Chen, P., Yuan, Y., Zhang, T., Xu, B., Gao, Q., Guan, T."Pentosan polysulfate ameliorates apoptosis and inflammation by suppressing activation of the p38 MAPK pathway in high glucose‑treated HK‑2 cells". International Journal of Molecular Medicine 41.2 (2018): 908-914.
Chicago
Chen, P., Yuan, Y., Zhang, T., Xu, B., Gao, Q., Guan, T."Pentosan polysulfate ameliorates apoptosis and inflammation by suppressing activation of the p38 MAPK pathway in high glucose‑treated HK‑2 cells". International Journal of Molecular Medicine 41, no. 2 (2018): 908-914. https://doi.org/10.3892/ijmm.2017.3290
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