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Epigallocatechin-3-gallate inhibits inflammation and epithelial‑mesenchymal transition through the PI3K/AKT pathway via upregulation of PTEN in asthma

  • Authors:
    • Nan Yang
    • Han Zhang
    • Xuxu Cai
    • Yunxiao Shang
  • View Affiliations / Copyright

    Affiliations: Department of Pediatrics, Shengjing Hospital of China Medical University, Shenyang, Liaoning 110004, P.R. China
    Copyright: © Yang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 818-828
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    Published online on: November 28, 2017
       https://doi.org/10.3892/ijmm.2017.3292
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Abstract

Asthma is a chronic disease associated with hyperresponsiveness, obstruction and remodeling of the airways. Epithelial-mesenchymal transition (EMT) has an important role in these alterations and may account for the accumulation of subepithelial mesenchymal cells, thus contributing to airway hyperresponsiveness and remodeling. Epigallo­catechin‑3‑gallate (EGCG), which is a type of polyphenol, is the most potent ingredient in green tea, and exhibits antibacterial, antiviral, antioxidative, anticancer and chemopreventive activities. Recently, numerous studies have investigated the protective effects of EGCG against asthma and other lung diseases. In the present study, the role of EGCG in ovalbumin (OVA)‑challenged asthmatic mice was determined. In addition, the inhibitory effects of EGCG against transforming growth factor (TGF)‑β1‑induced EMT and migration of 16HBE cells, and the underlying mechanisms of the phosphatidylinositol 3‑kinase/protein kinase B (PI3K/Akt) signaling pathway, were investigated by immunofluorescence, Transwell, wound healing assay and western blot analysis, respectively. The results indicated that EGCG may suppress inflammation and inflammatory cell infiltration into the lungs of OVA‑challenged asthmatic mice, and may also inhibit EMT via the PI3K/Akt signaling pathway through upregulating the expression of phosphatase and tensin homolog (PTEN) in vivo and in vitro. The present study also revealed the anti‑migratory effects of EGCG in TGF‑β1‑induced 16HBE cells, thus suggesting it may reduce airway remodeling. The present study provides a novel insight into understanding the protective effects of EGCG on airway remodeling in asthma, and indicates that EGCG may be useful as an adjuvant therapy for bronchial asthma.
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Copy and paste a formatted citation
Spandidos Publications style
Yang N, Zhang H, Cai X and Shang Y: Epigallocatechin-3-gallate inhibits inflammation and epithelial‑mesenchymal transition through the PI3K/AKT pathway via upregulation of PTEN in asthma. Int J Mol Med 41: 818-828, 2018.
APA
Yang, N., Zhang, H., Cai, X., & Shang, Y. (2018). Epigallocatechin-3-gallate inhibits inflammation and epithelial‑mesenchymal transition through the PI3K/AKT pathway via upregulation of PTEN in asthma. International Journal of Molecular Medicine, 41, 818-828. https://doi.org/10.3892/ijmm.2017.3292
MLA
Yang, N., Zhang, H., Cai, X., Shang, Y."Epigallocatechin-3-gallate inhibits inflammation and epithelial‑mesenchymal transition through the PI3K/AKT pathway via upregulation of PTEN in asthma". International Journal of Molecular Medicine 41.2 (2018): 818-828.
Chicago
Yang, N., Zhang, H., Cai, X., Shang, Y."Epigallocatechin-3-gallate inhibits inflammation and epithelial‑mesenchymal transition through the PI3K/AKT pathway via upregulation of PTEN in asthma". International Journal of Molecular Medicine 41, no. 2 (2018): 818-828. https://doi.org/10.3892/ijmm.2017.3292
Copy and paste a formatted citation
x
Spandidos Publications style
Yang N, Zhang H, Cai X and Shang Y: Epigallocatechin-3-gallate inhibits inflammation and epithelial‑mesenchymal transition through the PI3K/AKT pathway via upregulation of PTEN in asthma. Int J Mol Med 41: 818-828, 2018.
APA
Yang, N., Zhang, H., Cai, X., & Shang, Y. (2018). Epigallocatechin-3-gallate inhibits inflammation and epithelial‑mesenchymal transition through the PI3K/AKT pathway via upregulation of PTEN in asthma. International Journal of Molecular Medicine, 41, 818-828. https://doi.org/10.3892/ijmm.2017.3292
MLA
Yang, N., Zhang, H., Cai, X., Shang, Y."Epigallocatechin-3-gallate inhibits inflammation and epithelial‑mesenchymal transition through the PI3K/AKT pathway via upregulation of PTEN in asthma". International Journal of Molecular Medicine 41.2 (2018): 818-828.
Chicago
Yang, N., Zhang, H., Cai, X., Shang, Y."Epigallocatechin-3-gallate inhibits inflammation and epithelial‑mesenchymal transition through the PI3K/AKT pathway via upregulation of PTEN in asthma". International Journal of Molecular Medicine 41, no. 2 (2018): 818-828. https://doi.org/10.3892/ijmm.2017.3292
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