Effective component of Salvia miltiorrhiza in promoting cardiomyogenic differentiation of human placenta‑derived mesenchymal stem cells

  • Authors:
    • Kun Li
    • Jieqiong Song
    • Qian Zhao
    • Bo Wang
    • Yunli Zhang
    • Xuezhe Wang
    • Tiantian Tang
    • Shizheng Li
  • View Affiliations

  • Published online on: November 28, 2017     https://doi.org/10.3892/ijmm.2017.3293
  • Pages: 962-968
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Our previous study indicated that Salvia miltiorrhiza (SM) induced human placenta‑derived mesenchymal stem cells (hPDMSCs) to differentiate into cardiomyocytes, however, the effective component of SM in promoting cardiomyogenic differentiation remained to be elucidated. In the present study, the most commonly examined components of SM, including danshensu, salvianolic acid B, protocatechuic aldehyde, tanshinone I (TS I), TS IIA and cryptotanshinone, were used to determine the effective components of SM in promoting cardiomyogenic differentiation. The above components of SM slowed cell growth rate and altered cell morphology with a spindle or irregular shape to different degrees. The cells treated with the above components of SM showed increasing of cardiac protein expression to differing degrees, including GATA‑binding protein 4, atrial natriuretic factor, α‑sarcomeric actin and cardiac troponin‑I. Among the components of SM, TS IIA induced the most marked effects. In addition, the above components of SM increased the expression of phosphorylated glycogen synthase kinase‑3β, but decreased the expression of β‑catenin to different degrees, with TS IIA also having the most marked effects. In conclusion, the results of the present study suggested that TS IIA was the most effective active component of SM in inducing hPDMSCs to differentiate into cardiomyocytes, and that Wnt/β‑catenin signaling was important in the process of TS IIA promoting cardiomyogenic differentiation.
View Figures
View References

Related Articles

Journal Cover

February-2018
Volume 41 Issue 2

Print ISSN: 1107-3756
Online ISSN:1791-244X

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Li K, Song J, Zhao Q, Wang B, Zhang Y, Wang X, Tang T and Li S: Effective component of Salvia miltiorrhiza in promoting cardiomyogenic differentiation of human placenta‑derived mesenchymal stem cells. Int J Mol Med 41: 962-968, 2018
APA
Li, K., Song, J., Zhao, Q., Wang, B., Zhang, Y., Wang, X. ... Li, S. (2018). Effective component of Salvia miltiorrhiza in promoting cardiomyogenic differentiation of human placenta‑derived mesenchymal stem cells. International Journal of Molecular Medicine, 41, 962-968. https://doi.org/10.3892/ijmm.2017.3293
MLA
Li, K., Song, J., Zhao, Q., Wang, B., Zhang, Y., Wang, X., Tang, T., Li, S."Effective component of Salvia miltiorrhiza in promoting cardiomyogenic differentiation of human placenta‑derived mesenchymal stem cells". International Journal of Molecular Medicine 41.2 (2018): 962-968.
Chicago
Li, K., Song, J., Zhao, Q., Wang, B., Zhang, Y., Wang, X., Tang, T., Li, S."Effective component of Salvia miltiorrhiza in promoting cardiomyogenic differentiation of human placenta‑derived mesenchymal stem cells". International Journal of Molecular Medicine 41, no. 2 (2018): 962-968. https://doi.org/10.3892/ijmm.2017.3293