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Comparative analysis of human embryonic stem cell‑derived neural stem cells as an in vitro human model

  • Authors:
    • Jung‑Hwa Oh
    • Cho‑Rok Jung
    • Mi‑Ok Lee
    • Janghwan Kim
    • Mi‑Young Son
  • View Affiliations / Copyright

    Affiliations: Korea Institute of Toxicology, Daejeon 34114, Republic of Korea, Stem Cell Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 34141, Republic of Korea, Immunotherapy Convergence Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 34141, Republic of Korea
    Copyright: © Oh et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 783-790
    |
    Published online on: November 30, 2017
       https://doi.org/10.3892/ijmm.2017.3298
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Abstract

Alternative cell models of human neural stem cells (hNSCs) have been developed and used for investigations ranging from in vitro experiments to in vivo clinical studies. However, a cell model capable of mimicking the ʻnormalʼ state of hNSCs is mandatory in order to extrapolate the results of these studies to humans. In the present study, to select a more suitable hNSC model for developing human‑based experimental platforms, two representative hNSC types were compared, namely human embryonic stem cell (hESC)‑derived hNSCs and ReNcell CX cells, which are well‑characterized immortalized hNSC lines. The hNSCs, differentiated from hESCs via human neuroectodermal sphere (hNES) formation, recapitulated the molecular and cellular phenotypes of hNSCs, including NSC marker expression and terminal neuronal differentiation potential. Comparative analyses of the transcriptome profiles of the hESC‑derived hNESs and ReNcell CX hNSCs showed that the differentiated hNESs were analogous to the ReNcell CX cells, as demonstrated by principal component analysis and hierarchical sample clustering. The hNSC‑specific transcriptome was presented, comprising commonly expressed transcripts between hNESs derived from hESCs and ReNcell CX cells. To elucidate the molecular mechanisms associated with the hNSC identity, the hNSC‑specific transcriptome was analyzed using pathway and functional annotation clustering analyses. The results suggested that hESC‑derived hNESs, an expandable and accessible cell source, may be used as a relevant hNSC model in a wide range of neurological investigations.
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Copy and paste a formatted citation
Spandidos Publications style
Oh JH, Jung CR, Lee MO, Kim J and Son MY: Comparative analysis of human embryonic stem cell‑derived neural stem cells as an in vitro human model. Int J Mol Med 41: 783-790, 2018.
APA
Oh, J., Jung, C., Lee, M., Kim, J., & Son, M. (2018). Comparative analysis of human embryonic stem cell‑derived neural stem cells as an in vitro human model. International Journal of Molecular Medicine, 41, 783-790. https://doi.org/10.3892/ijmm.2017.3298
MLA
Oh, J., Jung, C., Lee, M., Kim, J., Son, M."Comparative analysis of human embryonic stem cell‑derived neural stem cells as an in vitro human model". International Journal of Molecular Medicine 41.2 (2018): 783-790.
Chicago
Oh, J., Jung, C., Lee, M., Kim, J., Son, M."Comparative analysis of human embryonic stem cell‑derived neural stem cells as an in vitro human model". International Journal of Molecular Medicine 41, no. 2 (2018): 783-790. https://doi.org/10.3892/ijmm.2017.3298
Copy and paste a formatted citation
x
Spandidos Publications style
Oh JH, Jung CR, Lee MO, Kim J and Son MY: Comparative analysis of human embryonic stem cell‑derived neural stem cells as an in vitro human model. Int J Mol Med 41: 783-790, 2018.
APA
Oh, J., Jung, C., Lee, M., Kim, J., & Son, M. (2018). Comparative analysis of human embryonic stem cell‑derived neural stem cells as an in vitro human model. International Journal of Molecular Medicine, 41, 783-790. https://doi.org/10.3892/ijmm.2017.3298
MLA
Oh, J., Jung, C., Lee, M., Kim, J., Son, M."Comparative analysis of human embryonic stem cell‑derived neural stem cells as an in vitro human model". International Journal of Molecular Medicine 41.2 (2018): 783-790.
Chicago
Oh, J., Jung, C., Lee, M., Kim, J., Son, M."Comparative analysis of human embryonic stem cell‑derived neural stem cells as an in vitro human model". International Journal of Molecular Medicine 41, no. 2 (2018): 783-790. https://doi.org/10.3892/ijmm.2017.3298
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