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Naringenin induces laxative effects by upregulating the expression levels of c-Kit and SCF, as well as those of aquaporin 3 in mice with loperamide-induced constipation

  • Authors:
    • Jianqiao Yin
    • Yichao Liang
    • Dalu Wang
    • Zhaopeng Yan
    • Hongzhuan Yin
    • Di Wu
    • Qi Su
  • View Affiliations / Copyright

    Affiliations: Department of General Surgery, Shengjing Hospital of China Medical University, Shenyang, Liaoning 110004, P.R. China
    Copyright: © Yin et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 649-658
    |
    Published online on: December 1, 2017
       https://doi.org/10.3892/ijmm.2017.3301
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Abstract

Constipation is a common affliction which causes discomfort and affects the quality of life of affected individuals. Naringenin (NAR), a natural flavonoid widely found in citrus fruits and tomatoes, has been reported to exhibit various pharmacological effects, such as anti-inflammatory, anti-atherogenic, anti-mutagenic, hepatoprotective and anticancer effects. Increasing evidence has indicated that NAR has potential for use in the treatment of constipation. Thus, the aim of this study was to evaluate the laxative effects of NAR in mice with loperamide-induced (Lop-induced) constipation. The data indicated that NAR relieved Lop-induced constipation in mice based on the changes of fecal parameters (numbers, weight and water content), the intestinal charcoal transit ratio and the histological alteration. ELISA revealed that NAR regulated the production levels of gastrointestinal metabolic components, such as motilin (MTL), gastrin (Gas), endothelin (ET), substance P (SP), acetylcholinesterase (AChE) and vasoactive intestinal peptide (VIP) in serum. The expression levels of enteric nerve-related factors, glial cell line-derived neurotrophic factor (GDNF), transient receptor potential vanilloid 1 (TRPV1), nitric oxide synthase (NOS), c-Kit, stem cell factor (SCF) and aquaporin 3 (AQP3) were examined by western blot analysis and RT-PCR analysis. The results of this study suggest that NAR relieves Lop-induced constipation by increasing the levels of interstitial cells of Cajal markers (c-Kit and SCF), as well as AQP3. Thus, NAR may be effective as a candidate in patients suffering from lifestyle-induced constipation.
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Copy and paste a formatted citation
Spandidos Publications style
Yin J, Liang Y, Wang D, Yan Z, Yin H, Wu D and Su Q: Naringenin induces laxative effects by upregulating the expression levels of c-Kit and SCF, as well as those of aquaporin 3 in mice with loperamide-induced constipation. Int J Mol Med 41: 649-658, 2018.
APA
Yin, J., Liang, Y., Wang, D., Yan, Z., Yin, H., Wu, D., & Su, Q. (2018). Naringenin induces laxative effects by upregulating the expression levels of c-Kit and SCF, as well as those of aquaporin 3 in mice with loperamide-induced constipation. International Journal of Molecular Medicine, 41, 649-658. https://doi.org/10.3892/ijmm.2017.3301
MLA
Yin, J., Liang, Y., Wang, D., Yan, Z., Yin, H., Wu, D., Su, Q."Naringenin induces laxative effects by upregulating the expression levels of c-Kit and SCF, as well as those of aquaporin 3 in mice with loperamide-induced constipation". International Journal of Molecular Medicine 41.2 (2018): 649-658.
Chicago
Yin, J., Liang, Y., Wang, D., Yan, Z., Yin, H., Wu, D., Su, Q."Naringenin induces laxative effects by upregulating the expression levels of c-Kit and SCF, as well as those of aquaporin 3 in mice with loperamide-induced constipation". International Journal of Molecular Medicine 41, no. 2 (2018): 649-658. https://doi.org/10.3892/ijmm.2017.3301
Copy and paste a formatted citation
x
Spandidos Publications style
Yin J, Liang Y, Wang D, Yan Z, Yin H, Wu D and Su Q: Naringenin induces laxative effects by upregulating the expression levels of c-Kit and SCF, as well as those of aquaporin 3 in mice with loperamide-induced constipation. Int J Mol Med 41: 649-658, 2018.
APA
Yin, J., Liang, Y., Wang, D., Yan, Z., Yin, H., Wu, D., & Su, Q. (2018). Naringenin induces laxative effects by upregulating the expression levels of c-Kit and SCF, as well as those of aquaporin 3 in mice with loperamide-induced constipation. International Journal of Molecular Medicine, 41, 649-658. https://doi.org/10.3892/ijmm.2017.3301
MLA
Yin, J., Liang, Y., Wang, D., Yan, Z., Yin, H., Wu, D., Su, Q."Naringenin induces laxative effects by upregulating the expression levels of c-Kit and SCF, as well as those of aquaporin 3 in mice with loperamide-induced constipation". International Journal of Molecular Medicine 41.2 (2018): 649-658.
Chicago
Yin, J., Liang, Y., Wang, D., Yan, Z., Yin, H., Wu, D., Su, Q."Naringenin induces laxative effects by upregulating the expression levels of c-Kit and SCF, as well as those of aquaporin 3 in mice with loperamide-induced constipation". International Journal of Molecular Medicine 41, no. 2 (2018): 649-658. https://doi.org/10.3892/ijmm.2017.3301
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