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Article

Mechanism of endogenous digitalis-like factor‑induced vascular endothelial cell damage in patients with severe preeclampsia

  • Authors:
    • Mei Peng
    • Mengyuan Yang
    • Yiling Ding
    • Ling Yu
    • Yali Deng
    • Weisi Lai
    • Yun Hu
  • View Affiliations / Copyright

    Affiliations: Department of Gynaecology and Obstetrics, The Second Xiangya Hospital of Central South University, Changsha, Hunan 410011, P.R. China
  • Pages: 985-994
    |
    Published online on: December 11, 2017
       https://doi.org/10.3892/ijmm.2017.3316
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Abstract

Although endogenous digitalis‑like factor (EDLF) is associated with the development of various physical disorders, the role in preeclampsia remains unclear. This study investigated the effects of EDLF on vascular endothelial cell damage in patients with preeclampsia and the potential mechanisms. From July 2014 to July 2015, 120 singleton pregnancy cases underwent a prenatal examination, inpatient delivery and had normal blood pressure were included in the study, either as patients with severe preeclampsia or the control patients. Serum EDLF levels were compared in these two groups, and an in vitro hypoxic trophocyte‑induced vascular endothelial cell damage model was established to explore the changes in hypoxic trophocyte EDLF level and the subsequent effects on human umbilical vein endothelial cells (HUVECs). Nuclear factor‑κB (NF‑κB) p65 gene expression was silenced in hypoxic trophocytes, and EDLF levels and HUVEC damage were subsequently assessed. Serum EDLF levels were significantly higher in the severe preeclampsia cases than in the controls at the same gestational week (P<0.001). EDLF levels in hypoxic trophocytes increased with the increasing co‑culture duration. Damage to the biofunctions of HUVECs co‑cultured with hypoxic trophocytes also increased with co‑culture duration. However, silencing of NF‑κB p65 in the hypoxic trophocytes reduced the EDLF levels. Annexin A2 was highly expressed in HUVECs, and no biofunctions were significantly damaged (P<0.05) compared with the group without receiving NF‑κB p65 silencing. Serum EDLF levels were significantly higher in patients with severe preeclampsia compared with the controls. The results of the current study indicate that NF‑κB p65 has a role in regulating EDLF production in hypoxic trophocytes.
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Copy and paste a formatted citation
Spandidos Publications style
Peng M, Yang M, Ding Y, Yu L, Deng Y, Lai W and Hu Y: Mechanism of endogenous digitalis-like factor‑induced vascular endothelial cell damage in patients with severe preeclampsia. Int J Mol Med 41: 985-994, 2018.
APA
Peng, M., Yang, M., Ding, Y., Yu, L., Deng, Y., Lai, W., & Hu, Y. (2018). Mechanism of endogenous digitalis-like factor‑induced vascular endothelial cell damage in patients with severe preeclampsia. International Journal of Molecular Medicine, 41, 985-994. https://doi.org/10.3892/ijmm.2017.3316
MLA
Peng, M., Yang, M., Ding, Y., Yu, L., Deng, Y., Lai, W., Hu, Y."Mechanism of endogenous digitalis-like factor‑induced vascular endothelial cell damage in patients with severe preeclampsia". International Journal of Molecular Medicine 41.2 (2018): 985-994.
Chicago
Peng, M., Yang, M., Ding, Y., Yu, L., Deng, Y., Lai, W., Hu, Y."Mechanism of endogenous digitalis-like factor‑induced vascular endothelial cell damage in patients with severe preeclampsia". International Journal of Molecular Medicine 41, no. 2 (2018): 985-994. https://doi.org/10.3892/ijmm.2017.3316
Copy and paste a formatted citation
x
Spandidos Publications style
Peng M, Yang M, Ding Y, Yu L, Deng Y, Lai W and Hu Y: Mechanism of endogenous digitalis-like factor‑induced vascular endothelial cell damage in patients with severe preeclampsia. Int J Mol Med 41: 985-994, 2018.
APA
Peng, M., Yang, M., Ding, Y., Yu, L., Deng, Y., Lai, W., & Hu, Y. (2018). Mechanism of endogenous digitalis-like factor‑induced vascular endothelial cell damage in patients with severe preeclampsia. International Journal of Molecular Medicine, 41, 985-994. https://doi.org/10.3892/ijmm.2017.3316
MLA
Peng, M., Yang, M., Ding, Y., Yu, L., Deng, Y., Lai, W., Hu, Y."Mechanism of endogenous digitalis-like factor‑induced vascular endothelial cell damage in patients with severe preeclampsia". International Journal of Molecular Medicine 41.2 (2018): 985-994.
Chicago
Peng, M., Yang, M., Ding, Y., Yu, L., Deng, Y., Lai, W., Hu, Y."Mechanism of endogenous digitalis-like factor‑induced vascular endothelial cell damage in patients with severe preeclampsia". International Journal of Molecular Medicine 41, no. 2 (2018): 985-994. https://doi.org/10.3892/ijmm.2017.3316
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