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Article Open Access

shRNA-induced silencing of Ras-related C3 botulinum toxin substrate 1 inhibits the proliferation of colon cancer cells through upregulation of BAD and downregulation of cyclin D1

  • Authors:
    • You‑Sheng Huang
    • Na Jie
    • Yi‑Xin Zhang
    • Ke‑Jian Zou
    • Yang Weng
  • View Affiliations / Copyright

    Affiliations: Department of Pathology, The First Affiliated Hospital of Hainan Medical University, Hainan Medical University, Haikou, Hainan 571101, P.R. China, Department of Gastrointestinal Surgery, Hainan Provincial People's Hospital, Haikou, Hainan 570311, P.R. China
    Copyright: © Huang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1397-1408
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    Published online on: December 22, 2017
       https://doi.org/10.3892/ijmm.2017.3345
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Abstract

Ras-related C3 botulinum toxin substrate 1 (RAC1) is a member of the Rho family of small GTPases. Recent studies have reported that RAC1 serves an important role in colon cancer cell proliferation. The present study aimed to investigate the effects of RAC1 knockdown on cell proliferation, cell cycle progression and apoptosis of colon cancer cells. Lentivirus‑mediated short hairpin RNA (shRNA) was used to knockdown RAC1 expression in colon cancer cell lines, and cell proliferation, apoptosis, cell cycle progression were evaluated by MTT assays and flow cytometry. The differences in mRNAs expression were identified between RAC1-knockdown cells and control cells using a mRNA microarray, following which quantitative PCR (qPCR) and western blot were employed to confirm the results of the mRNA microarray. The proliferative ability of colon cancer cells was significantly decreased following RAC1 knockdown, and RAC1 knockdown increased the apoptotic rate and enhanced cell cycle arrest at G1 phase in colon cancer cells. In addition, >1,200 known genes were demonstrated to be involved in RAC1‑associated tumorigenic functions in SW620 colon cancer cells, as determined by gene chip analysis; these genes were associated with cell proliferation, cell cycle, apoptosis and metastasis. Furthermore, western blot analysis indicated that cyclin D1 was downregulated, whereas B‑cell lymphoma 2‑associated agonist of cell death (BAD) was upregulated following RAC1 knockdown in colon cancer cells. In conclusion, RAC1 silencing may suppress the proliferation of colon cancer cells by inducing apoptosis and cell cycle arrest. In addition, a large number of genes were revealed to be involved in the process, including BAD, which was upregulated and cyclin D1, which was downregulated. Further studies on these differentially expressed genes may provide a better understanding of the potential roles of RAC1 in colon carcinogenesis.
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Copy and paste a formatted citation
Spandidos Publications style
Huang YS, Jie N, Zhang YX, Zou KJ and Weng Y: shRNA-induced silencing of Ras-related C3 botulinum toxin substrate 1 inhibits the proliferation of colon cancer cells through upregulation of BAD and downregulation of cyclin D1. Int J Mol Med 41: 1397-1408, 2018.
APA
Huang, Y., Jie, N., Zhang, Y., Zou, K., & Weng, Y. (2018). shRNA-induced silencing of Ras-related C3 botulinum toxin substrate 1 inhibits the proliferation of colon cancer cells through upregulation of BAD and downregulation of cyclin D1. International Journal of Molecular Medicine, 41, 1397-1408. https://doi.org/10.3892/ijmm.2017.3345
MLA
Huang, Y., Jie, N., Zhang, Y., Zou, K., Weng, Y."shRNA-induced silencing of Ras-related C3 botulinum toxin substrate 1 inhibits the proliferation of colon cancer cells through upregulation of BAD and downregulation of cyclin D1". International Journal of Molecular Medicine 41.3 (2018): 1397-1408.
Chicago
Huang, Y., Jie, N., Zhang, Y., Zou, K., Weng, Y."shRNA-induced silencing of Ras-related C3 botulinum toxin substrate 1 inhibits the proliferation of colon cancer cells through upregulation of BAD and downregulation of cyclin D1". International Journal of Molecular Medicine 41, no. 3 (2018): 1397-1408. https://doi.org/10.3892/ijmm.2017.3345
Copy and paste a formatted citation
x
Spandidos Publications style
Huang YS, Jie N, Zhang YX, Zou KJ and Weng Y: shRNA-induced silencing of Ras-related C3 botulinum toxin substrate 1 inhibits the proliferation of colon cancer cells through upregulation of BAD and downregulation of cyclin D1. Int J Mol Med 41: 1397-1408, 2018.
APA
Huang, Y., Jie, N., Zhang, Y., Zou, K., & Weng, Y. (2018). shRNA-induced silencing of Ras-related C3 botulinum toxin substrate 1 inhibits the proliferation of colon cancer cells through upregulation of BAD and downregulation of cyclin D1. International Journal of Molecular Medicine, 41, 1397-1408. https://doi.org/10.3892/ijmm.2017.3345
MLA
Huang, Y., Jie, N., Zhang, Y., Zou, K., Weng, Y."shRNA-induced silencing of Ras-related C3 botulinum toxin substrate 1 inhibits the proliferation of colon cancer cells through upregulation of BAD and downregulation of cyclin D1". International Journal of Molecular Medicine 41.3 (2018): 1397-1408.
Chicago
Huang, Y., Jie, N., Zhang, Y., Zou, K., Weng, Y."shRNA-induced silencing of Ras-related C3 botulinum toxin substrate 1 inhibits the proliferation of colon cancer cells through upregulation of BAD and downregulation of cyclin D1". International Journal of Molecular Medicine 41, no. 3 (2018): 1397-1408. https://doi.org/10.3892/ijmm.2017.3345
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