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International Journal of Molecular Medicine
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miR-133b reverses cisplatin resistance by targeting GSTP1 in cisplatin-resistant lung cancer cells

  • Authors:
    • Chen Lin
    • Liyi Xie
    • Yan Lu
    • Zhihuang Hu
    • Jianhua Chang
  • View Affiliations / Copyright

    Affiliations: Department of Oncology, Shanghai Medical College, Fudan University, Shanghai 200032, P.R. China
    Copyright: © Lin et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2050-2058
    |
    Published online on: January 11, 2018
       https://doi.org/10.3892/ijmm.2018.3382
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Abstract

MicroRNAs play a critical role in chemoresistance and are implicated in various biological and pathological processes of cells. The objective of the present study was to explore the role of miR‑133b and its mechanism in the regulation of cisplatin resistance and tumor progression in cisplatin‑resistant non‑small cell lung cancer (NSCLC) cells. Reverse transcription‑quantitative polymerase chain reaction and western blot assays of the cisplatin‑resistant cell lines A549/DPP and H1299/DDP displayed the reduced expression of miR‑133b and increased expression of glutathione-S-transferase P1 (GSTP1) in the resistant cells compared with the respective parental cell lines A549 and H1299. Cell Counting kit‑8, flow cytometry, colony formation and Transwell migration assays indicated that the overexpression of miR‑133b increased the chemosensitivity to cisplatin and attenuated the proliferation and migration capacities of the cisplatin‑resistant NSCLC cell lines in vitro. A dual‑luciferase reporter assay demonstrated that miR‑133b negatively regulated the expression of GSTP1 by targeting its 3'‑untranslated region. In addition, the knockdown of GSTP1 by transfection with small interfering RNA exerted similar effects on cell chemosensitivity, proliferation and migration as did ectopic miR‑133b expression, in addition to the upregulation of Bax and downregulation of Bcl‑2, survivin and matrix metalloproteinase expression. In conclusion, the present study findings provide the insights that miR‑133b reduces cisplatin resistance and its overexpression contributes to the suppression of the malignant growth and aggressiveness of cisplatin‑resistant NSCLC cells by targeting GSTP1. This could potentially be exploited as a novel therapeutic strategy for the reversal of cisplatin resistance.
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Copy and paste a formatted citation
Spandidos Publications style
Lin C, Xie L, Lu Y, Hu Z and Chang J: miR-133b reverses cisplatin resistance by targeting GSTP1 in cisplatin-resistant lung cancer cells. Int J Mol Med 41: 2050-2058, 2018.
APA
Lin, C., Xie, L., Lu, Y., Hu, Z., & Chang, J. (2018). miR-133b reverses cisplatin resistance by targeting GSTP1 in cisplatin-resistant lung cancer cells. International Journal of Molecular Medicine, 41, 2050-2058. https://doi.org/10.3892/ijmm.2018.3382
MLA
Lin, C., Xie, L., Lu, Y., Hu, Z., Chang, J."miR-133b reverses cisplatin resistance by targeting GSTP1 in cisplatin-resistant lung cancer cells". International Journal of Molecular Medicine 41.4 (2018): 2050-2058.
Chicago
Lin, C., Xie, L., Lu, Y., Hu, Z., Chang, J."miR-133b reverses cisplatin resistance by targeting GSTP1 in cisplatin-resistant lung cancer cells". International Journal of Molecular Medicine 41, no. 4 (2018): 2050-2058. https://doi.org/10.3892/ijmm.2018.3382
Copy and paste a formatted citation
x
Spandidos Publications style
Lin C, Xie L, Lu Y, Hu Z and Chang J: miR-133b reverses cisplatin resistance by targeting GSTP1 in cisplatin-resistant lung cancer cells. Int J Mol Med 41: 2050-2058, 2018.
APA
Lin, C., Xie, L., Lu, Y., Hu, Z., & Chang, J. (2018). miR-133b reverses cisplatin resistance by targeting GSTP1 in cisplatin-resistant lung cancer cells. International Journal of Molecular Medicine, 41, 2050-2058. https://doi.org/10.3892/ijmm.2018.3382
MLA
Lin, C., Xie, L., Lu, Y., Hu, Z., Chang, J."miR-133b reverses cisplatin resistance by targeting GSTP1 in cisplatin-resistant lung cancer cells". International Journal of Molecular Medicine 41.4 (2018): 2050-2058.
Chicago
Lin, C., Xie, L., Lu, Y., Hu, Z., Chang, J."miR-133b reverses cisplatin resistance by targeting GSTP1 in cisplatin-resistant lung cancer cells". International Journal of Molecular Medicine 41, no. 4 (2018): 2050-2058. https://doi.org/10.3892/ijmm.2018.3382
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