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Article

NOX4/ROS mediate ethanol‑induced apoptosis via MAPK signal pathway in L‑02 cells

  • Authors:
    • Cheng‑Fang Yang
    • Yu‑Juan Zhong
    • Zuheng Ma
    • Li Li
    • Lin Shi
    • Li Chen
    • Chen Li
    • Dan Wu
    • Qi Chen
    • Yong‑Wen Li
  • View Affiliations / Copyright

    Affiliations: Department of Pharmacology, College of Pharmacy, Guilin Medical University, Guilin, Guangxi 541004, P.R. China, Department of Molecular Medicine and Surgery, Karolinska Institutet, Stockholm SE‑171 76, Sweden
  • Pages: 2306-2316
    |
    Published online on: January 16, 2018
       https://doi.org/10.3892/ijmm.2018.3390
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Abstract

The aim of the present study was to assess the molecular mechanism of ethanol‑induced oxidative stress‑mediated apoptosis in L‑02 liver cells in order to elucidate novel pathways associated with alcoholic liver disease. L‑02 cells were treated with 400 mM ethanol with or without inhibitors. The cell viability was measured by an MTT assay. Cell apoptosis was assessed by flow cytometry and a single‑stranded DNA (ssDNA) assay. Intracellular reactive oxygen species (ROS) production of L‑02 cells was determined using the 2',7'‑dichlorofluorescein‑diacetate dye. The protein expression of c‑Jun N‑terminal kinase (JNK), phosphorylated (p)‑JNK, P38, p‑P38, NADPH oxidase (NOX)1, NOX4, p22phox, B‑cell lymphoma 2 (Bcl‑2) and Bcl‑2‑associated X protein were measured by western blot analysis. The mRNA expression of NOX1, NOX4 and p22phox was measured by reverse transcription polymerase chain reaction analysis. The results indicated that after treatment with various concentrations of ethanol for the indicated durations, L‑02 cells were displayed a significant decrease in cell viability in a dose‑and time‑dependent manner. Ethanol‑induced apoptosis and cell death of L‑02 cells was accompanied by the generation of ROS, elevated expression of NOX, as well as phosphorylation of JNK and P‑38. In addition, increased expression of Bcl‑2 was induced by 400 mM ethanol. Furthermore, treatment with NOX inhibitor attenuated the ethanol‑induced a decrease in cell viability, and an increase in apoptosis and Bcl‑2 expression. In conclusion, ethanol induced apoptosis in the L‑02 hepatocyte cell line via generation of ROS and elevated expression of NOX4. This indicated that activation of JNK and p38 in the mitogen‑activated protein kinase pathway promotes apoptosis in L‑02 cells.
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View References

1 

Duncan C: Rethinking excessive habits and addictive behaviors. Alcohol Alcohol. 52:128–129. 2017. View Article : Google Scholar

2 

Owens RE, Snyder HS, Twilla JD and Satapathy SK: Pharmacologic treatment of alcoholic hepatitis: Examining outcomes based on disease severity stratification. J Clin Exp Hepatol. 6:275–281. 2016. View Article : Google Scholar : PubMed/NCBI

3 

Kim JW, Yang H, Kim HW, Kim HP and Sung SH: Lignans from Opuntia ficusindica seeds protect rat primary hepatocytes and HepG2 cells against ethanol-induced oxidative stress. Biosci Biotechnol Biochem. 81:181–183. 2017. View Article : Google Scholar

4 

Sun Q, Zhang W, Zhong W, Sun X and Zhou Z: Pharmacological inhibition of NOX4 ameliorates alcohol-induced liver injury in mice through improving oxidative stress and mitochondrial function. Biochim Biophys Acta. 1861:2912–2921. 2017. View Article : Google Scholar :

5 

Zhang F, Wang X, Qiu X, Wang J, Fang H, Wang Z, Sun Y and Xia Z: The protective effect of esculentoside a on experimental acute liver injury in mice. PLoS One. 9:e1131072014. View Article : Google Scholar : PubMed/NCBI

6 

Sinha K, Das J, Pal PB and Sil PC: Oxidative stress: The mitochondria-dependent and mitochondria-independent pathways of apoptosis. Arch Toxicol. 87:1157–1180. 2013. View Article : Google Scholar : PubMed/NCBI

7 

Kim EK and Choi EJ: Compromised MAPK signaling in human diseases: An update. Arch Toxicol. 89:867–882. 2015. View Article : Google Scholar : PubMed/NCBI

8 

Wang Y, Wang R, Wang Y, Peng R, Wu Y and Yuan Y: Ginkgo biloba extract mitigates liver fibrosis and apoptosis by regulating p38 MAPK, NF-κB/IκBα, and Bcl-2/Bax signaling. Drug Des Devel Ther. 9:6303–6317. 2015.

9 

Crosas-Molist E and Fabregat I: Role of NADPH oxidases in the redox biology of liver fibrosis. Redox Biol. 6:106–111. 2015. View Article : Google Scholar : PubMed/NCBI

10 

Ma JQ, Ding J, Zhang L and Liu CM: Hepatoprotective properties of sesamin against CCl4 induced oxidative stress-mediatedapoptosisin mice via JNK pathway. Food Chem Toxicol. 64:41–48. 2014. View Article : Google Scholar

11 

Morio Y, Tsuji M, Inagaki M, Nakagawa M, Asaka Y, Oyamada H, Furuya K and Oguchi K: Ethanol-induced apoptosis in human liver adenocarcinoma cells (SK-Hep1): Fas- and mitochondria-mediated pathways and interaction with MAPK signaling system. Toxicol In Vitro. 27:1820–1829. 2013. View Article : Google Scholar : PubMed/NCBI

12 

Guo X, Cui R, Zhao J, Mo R, Peng L and Yan M: Corosolic acid protects hepatocytes against ethanol-induced damage by modulating mitogen-activated protein kinases and activating autophagy. Eur J Pharmacol. 791:578–588. 2016. View Article : Google Scholar : PubMed/NCBI

13 

Liang S, Su WW, Wang YG, Peng W, Nie YC and Li PB: Effect of quercetin 7-rhamnoside on glycochenodeoxycholic acid-induced L-02 human normal liver cell apoptosis. Int J Mol Med. 32:323–330. 2013. View Article : Google Scholar : PubMed/NCBI

14 

Liu LQ, Fan ZQ, Tang YF and Ke ZJ: The resveratrol attenuates ethanol-induced hepatocyte apoptosis via inhibiting ER-related caspase-12 activation and PDE activity in vitro. Alcohol Clin Exp Res. 38:683–693. 2014. View Article : Google Scholar

15 

Andreu-Fernández V, Sancho M, Genovés A, Lucendo E, Todt F, Lauterwasser J, Funk K, Jahreis G, Pérez-Payá E, Mingarro I, et al: Bax transmembrane domain interacts with prosurvival Bcl-2 proteins in biological membranes. Proc Natl Acad Sci USA. 114:310–315. 2017. View Article : Google Scholar :

16 

Farshori NN, Al-Sheddi ES, Al-Oqail MM, Hassan WH, Al-Khedhairy AA, Musarrat J and Siddiqui MA: Hepatoprotective potential of Lavandula coronopifolia extracts against ethanol induced oxidative stress-mediated cytotoxicity in HepG2 cells. Toxicol Ind Health. 31:727–737. 2015. View Article : Google Scholar

17 

Liang S, Kisseleva T and Brenner DA: The role of NADPH oxidases (NOXs) in liver fibrosis and the activation of myofibro-blasts. Front Physiol. 7:172016. View Article : Google Scholar

18 

Jiang JX and Török NJ: NADPH oxidases in chronic liver diseases. Adv Hepatol. 2014:pii 7429312014. View Article : Google Scholar

19 

Jiao Y, Ji L, Kuang Y and Yang Q: Cytotoxic effect of oxaloacetate on HepG2-human hepatic carcinoma cells via apoptosis and ROS accumulation. Neoplasma. 64:192–198. 2017. View Article : Google Scholar : PubMed/NCBI

20 

Bost ER, Frye GS, Ahn B and Ferreira LF: Diaphragm dysfunction caused by sphingomyelinase requires the p47(phox) subunit of NADPH oxidase. Respir Physiol Neurobiol. 205:47–52. 2015. View Article : Google Scholar

21 

Li YY, Shi ZM, Yu XY, Feng P and Wang XJ: Urotensin II-induced insulin resistance is mediated by NADPH oxidase-derived reactive oxygen species in HepG2 cells. World J Gastroenterol. 22:5769–5779. 2016. View Article : Google Scholar : PubMed/NCBI

22 

Liu J, Wei X, Wu Y, Wang Y, Qiu Y, Shi J, Zhou H, Lu Z, Shao M, Yu L and Tong L: Giganteaside D induces ROS-mediated apoptosis in human hepatocellular carcinoma cells through the MAPK pathway. Cell Oncol (Dordr). 39:333–342. 2016. View Article : Google Scholar

23 

He Y, Yang J, Li H, Shao H, Wei C, Wang Y, Li M and Xu C: Exogenous spermine ameliorates high glucose-induced cardiomyocytic apoptosis via decreasing reactive oxygen species accumulation through inhibiting p38/JNK andJAK2 pathways. Int J Clin Exp Pathol. 8:15537–15549. 2015.

24 

Zhang C, Jia X, Bao J, Chen S, Wang K, Zhang Y, Li P, Wan JB, Su H, Wang Y, et al: Polyphyllin VII induces apoptosis in HepG2 cells through ROS-mediated mitochondrial dysfunction and MAPK pathways. BMC Complement Altern Med. 16:582016. View Article : Google Scholar : PubMed/NCBI

25 

Wang Y, Sun Z, Chen S, Jiao Y and Bai C: ROS-mediated activation of JNK/p38 contributes partially to the pro-apoptotic effect of ajoene on cells of lung adenocarcinoma. Tumour Biol. 37:3727–3738. 2016. View Article : Google Scholar

26 

Zhang Q, Cui C, Chen CQ, Hu XL, Liu YH, Fan YH, Meng WH and Zhao QC: Anti-proliferative and pro-apoptotic activities of Alpinia oxyphylla on HepG2 cells through ROS-mediated signaling pathway. J Ethnopharmacol. 169:99–108. 2015. View Article : Google Scholar : PubMed/NCBI

27 

Liang B, Liu Z, Cao Y, Zhu C, Zuo Y, Huang L, Wen G, Shang N, Chen Y, Yue X, et al: MC37, a new mono-carbonyl curcumin analog, induces G2/M cell cycle arrest and mitochondria-mediated apoptosis in human colorectal cancer cells. Eur J Pharmacol. 796:139–148. 2017. View Article : Google Scholar

28 

Chen J, Wang Y, Hui C, Xi Y, Liu X, Qi F, Liu H, Wang Z and Niu S: Mechanisms of Heshouwuyin in regulating apoptosis of testicular cells in aging rats through mitochondrial pathway. BMC Complement Altern Med. 16:3372016. View Article : Google Scholar : PubMed/NCBI

29 

Yan X, Jiang Z, Bi L, Yang Y and Chen W: Salvianolic acid A attenuates TNF-α- and D-GalN-induced ER stress-mediated and mitochondrial-dependent apoptosis by modulating Bax/Bcl-2 ratio and calcium release in hepatocyte LO2 cells. Naunyn Schmiedebergs Arch Pharmacol. 388:817–830. 2015. View Article : Google Scholar : PubMed/NCBI

30 

Yang Y, Zong M, Xu W, Zhang Y, Wang B, Yang M and Tao L: Natural pyrethrins induces apoptosis in human hepatocyte cells via Bax- and Bcl-2-mediated mitochondrial pathway. Chem Biol Interact. 262:38–45. 2017. View Article : Google Scholar

31 

Chen LY, Chen Q, Zhu XJ, Kong DS, Wu L, Shao JJ and Zheng SZ: Diallyl trisulfide protects against ethanol-induced oxidative stress and apoptosis via a hydrogen sulfide-mediated mechanism. Int Immunopharmacol. 36:23–30. 2016. View Article : Google Scholar : PubMed/NCBI

32 

Sumegi K, Fekete K, Antus C, Debreceni B, Hocsak E, Gallyas F Jr, Sumegi B and Szabo A: BGP-15 protects against oxidative stress- or lipopolysaccharide-induced mitochondrial destabilization and reduces mitochondrial production of reactive oxygen species. PLoS One. 12:e01693722017. View Article : Google Scholar : PubMed/NCBI

33 

Yan F, Wang Y, Wu X, Peshavariya HM, Dusting GJ, Zhang M and Jiang F: Nox4 and redox signaling mediate TGF-β-induced endothelial cell apoptosis and phenotypic switch. Cell Death Dis. 5:e10102014. View Article : Google Scholar

34 

Amatore D, Sgarbanti R, Aquilano K, Baldelli S, Limongi D, Civitelli L, Nencioni L, Garaci E, Ciriolo MR and Palamara AT: Influenza virus replication in lung epithelial cells depends on redox-sensitive pathways activated by NOX4-derived ROS. Cell Microbiol. 17:131–145. 2015. View Article : Google Scholar :

35 

Chuang WL, Lin PY, Lin HC and Chen YL: The apoptotic effect of ursolic acid on SK-Hep-1 cells is regulated by the I3K/Akt, p38 and JNK mapk signaling pathways. Molecules. 21:4602016. View Article : Google Scholar

36 

Paik YH, Kim J, Aoyama T, De Minicis S, Bataller R and Brenner DA: Role of NADPH oxidases in liver fibrosis. Antioxid Redox Signal. 20:2854–2872. 2014. View Article : Google Scholar :

37 

Nguyen KC, Willmore WG and Tayabali AF: Cadmium telluride quantum dots cause oxidative stress leading to extrinsic and intrinsic apoptosis in hepatocellular carcinoma HepG2 cells. Toxicology. 306:114–123. 2013. View Article : Google Scholar : PubMed/NCBI

38 

Zhao WX, Tang SS, Jin X, Zhang CM, Zhang T, Wang CC, Sun Y and Xiao XL: Olaquindox-induced apoptosis is suppressed through p38 MAPK and ROS-mediated JNK pathways in HepG2 cells. Cell Biol Toxicol. 29:229–238. 2013. View Article : Google Scholar : PubMed/NCBI

39 

Yoon J, Ham H, Sung J, Kim Y, Choi Y, Lee JS, Jeong HS, Lee J and Kim D: Black rice extract protected HepG2 cells from oxidative stress-induced cell death via ERK1/2 and Akt activation. Nutr Res Pract. 8:125–131. 2014. View Article : Google Scholar : PubMed/NCBI

40 

Stiuso P, Bagarolo ML, Ilisso CP, Vanacore D, Martino E, Caraglia M, Porcelli M and Cacciapuoti G: Protective effect of tyrosol and S-Adenosylmethionine against ethanol-induced oxidative stress of Hepg2 cells involves sirtuin 1, P53 and Erk1/2 Signaling. Int J Mol Sci. 17:pii: E6222016. View Article : Google Scholar

41 

Szuster-Ciesielska A, Plewka K, Daniluk J and Kandefer-Szerszeń M: Zinc inhibits ethanol-induced HepG2 cell apoptosis. Toxicol Appl Pharmacol. 229:1–9. 2008. View Article : Google Scholar : PubMed/NCBI

42 

Abid MD, Chen J, Xiang M, Zhou J, Chen X and Gong F: Khat (Catha edulis) generates reactive oxygen species and promotes hepatic cell apoptosis via MAPK activation. Int J Mol Med. 32:389–395. 2013. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Yang CF, Zhong YJ, Ma Z, Li L, Shi L, Chen L, Li C, Wu D, Chen Q, Li YW, Li YW, et al: NOX4/ROS mediate ethanol‑induced apoptosis via MAPK signal pathway in L‑02 cells. Int J Mol Med 41: 2306-2316, 2018.
APA
Yang, C., Zhong, Y., Ma, Z., Li, L., Shi, L., Chen, L. ... Li, Y. (2018). NOX4/ROS mediate ethanol‑induced apoptosis via MAPK signal pathway in L‑02 cells. International Journal of Molecular Medicine, 41, 2306-2316. https://doi.org/10.3892/ijmm.2018.3390
MLA
Yang, C., Zhong, Y., Ma, Z., Li, L., Shi, L., Chen, L., Li, C., Wu, D., Chen, Q., Li, Y."NOX4/ROS mediate ethanol‑induced apoptosis via MAPK signal pathway in L‑02 cells". International Journal of Molecular Medicine 41.4 (2018): 2306-2316.
Chicago
Yang, C., Zhong, Y., Ma, Z., Li, L., Shi, L., Chen, L., Li, C., Wu, D., Chen, Q., Li, Y."NOX4/ROS mediate ethanol‑induced apoptosis via MAPK signal pathway in L‑02 cells". International Journal of Molecular Medicine 41, no. 4 (2018): 2306-2316. https://doi.org/10.3892/ijmm.2018.3390
Copy and paste a formatted citation
x
Spandidos Publications style
Yang CF, Zhong YJ, Ma Z, Li L, Shi L, Chen L, Li C, Wu D, Chen Q, Li YW, Li YW, et al: NOX4/ROS mediate ethanol‑induced apoptosis via MAPK signal pathway in L‑02 cells. Int J Mol Med 41: 2306-2316, 2018.
APA
Yang, C., Zhong, Y., Ma, Z., Li, L., Shi, L., Chen, L. ... Li, Y. (2018). NOX4/ROS mediate ethanol‑induced apoptosis via MAPK signal pathway in L‑02 cells. International Journal of Molecular Medicine, 41, 2306-2316. https://doi.org/10.3892/ijmm.2018.3390
MLA
Yang, C., Zhong, Y., Ma, Z., Li, L., Shi, L., Chen, L., Li, C., Wu, D., Chen, Q., Li, Y."NOX4/ROS mediate ethanol‑induced apoptosis via MAPK signal pathway in L‑02 cells". International Journal of Molecular Medicine 41.4 (2018): 2306-2316.
Chicago
Yang, C., Zhong, Y., Ma, Z., Li, L., Shi, L., Chen, L., Li, C., Wu, D., Chen, Q., Li, Y."NOX4/ROS mediate ethanol‑induced apoptosis via MAPK signal pathway in L‑02 cells". International Journal of Molecular Medicine 41, no. 4 (2018): 2306-2316. https://doi.org/10.3892/ijmm.2018.3390
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