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Article

Engineered zinc-finger transcription factors inhibit the replication and transcription of HBV in vitro and in vivo

  • Authors:
    • Wei Luo
    • Junxia Wang
    • Dengfeng Xu
    • Huili Bai
    • Yangli Zhang
    • Yuhong Zhang
    • Xiaosong Li
  • View Affiliations / Copyright

    Affiliations: Department of General Surgery, The Second Affiliated Hospital, Southwest Medical University, Luzhou, Sichuan 646000, P.R. China, Department of Neonatology, The Second Affiliated Hospital, Southwest Medical University, Luzhou, Sichuan 646000, P.R. China, Department of Ophthalmology, Chongqing General Hospital, Chongqing 400014, P.R. China, Department of Molecular Diagnostics, Center for Clinical Molecular Medical Detection, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, P.R. China
  • Pages: 2169-2176
    |
    Published online on: January 17, 2018
       https://doi.org/10.3892/ijmm.2018.3396
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Abstract

In the present study, an artificial zinc-finger transcription factor eukaryotic expression vector specifically recognizing and binding to the hepatitis B virus (HBV) enhancer (Enh) was constructed, which inhibited the replication and expression of HBV DNA. The HBV EnhI‑specific pcDNA3.1‑artificial transcription factor (ATF) vector was successfully constructed, and then transformed or injected into HepG2.2.15 cells and HBV transgenic mice, respectively. The results demonstrated that the HBV EnhI (1,070‑1,234 bp)‑specific ATF significantly inhibited the replication and transcription of HBV DNA in vivo and in vitro. The HBV EnhI‑specific ATF may be a meritorious component of progressive combination therapies for eliminating HBV DNA in infected patients. A radical cure for chronic HBV infection may become feasible by using this bioengineering technology.
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Copy and paste a formatted citation
Spandidos Publications style
Luo W, Wang J, Xu D, Bai H, Zhang Y, Zhang Y and Li X: Engineered zinc-finger transcription factors inhibit the replication and transcription of HBV in vitro and in vivo. Int J Mol Med 41: 2169-2176, 2018.
APA
Luo, W., Wang, J., Xu, D., Bai, H., Zhang, Y., Zhang, Y., & Li, X. (2018). Engineered zinc-finger transcription factors inhibit the replication and transcription of HBV in vitro and in vivo. International Journal of Molecular Medicine, 41, 2169-2176. https://doi.org/10.3892/ijmm.2018.3396
MLA
Luo, W., Wang, J., Xu, D., Bai, H., Zhang, Y., Zhang, Y., Li, X."Engineered zinc-finger transcription factors inhibit the replication and transcription of HBV in vitro and in vivo". International Journal of Molecular Medicine 41.4 (2018): 2169-2176.
Chicago
Luo, W., Wang, J., Xu, D., Bai, H., Zhang, Y., Zhang, Y., Li, X."Engineered zinc-finger transcription factors inhibit the replication and transcription of HBV in vitro and in vivo". International Journal of Molecular Medicine 41, no. 4 (2018): 2169-2176. https://doi.org/10.3892/ijmm.2018.3396
Copy and paste a formatted citation
x
Spandidos Publications style
Luo W, Wang J, Xu D, Bai H, Zhang Y, Zhang Y and Li X: Engineered zinc-finger transcription factors inhibit the replication and transcription of HBV in vitro and in vivo. Int J Mol Med 41: 2169-2176, 2018.
APA
Luo, W., Wang, J., Xu, D., Bai, H., Zhang, Y., Zhang, Y., & Li, X. (2018). Engineered zinc-finger transcription factors inhibit the replication and transcription of HBV in vitro and in vivo. International Journal of Molecular Medicine, 41, 2169-2176. https://doi.org/10.3892/ijmm.2018.3396
MLA
Luo, W., Wang, J., Xu, D., Bai, H., Zhang, Y., Zhang, Y., Li, X."Engineered zinc-finger transcription factors inhibit the replication and transcription of HBV in vitro and in vivo". International Journal of Molecular Medicine 41.4 (2018): 2169-2176.
Chicago
Luo, W., Wang, J., Xu, D., Bai, H., Zhang, Y., Zhang, Y., Li, X."Engineered zinc-finger transcription factors inhibit the replication and transcription of HBV in vitro and in vivo". International Journal of Molecular Medicine 41, no. 4 (2018): 2169-2176. https://doi.org/10.3892/ijmm.2018.3396
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