Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
International Journal of Molecular Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1107-3756 Online ISSN: 1791-244X
Journal Cover
April-2018 Volume 41 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
April-2018 Volume 41 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

CTHRC1 mediates IL‑1β‑induced apoptosis in chondrocytes via JNK1/2 signaling

  • Authors:
    • Qi Zhang
    • Zong‑Sheng Yin
    • Fu‑Wen Zhang
    • Kun Cao
    • He‑Yan Sun
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedics, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230022, P.R. China
  • Pages: 2270-2278
    |
    Published online on: January 18, 2018
       https://doi.org/10.3892/ijmm.2018.3403
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Osteoarthritis (OA), also known as degenerative joint disease or degenerative arthritis, is characterized by chondrocyte apoptosis. The aim of the present study was to investigate the effects of collagen triple helix repeat containing 1 (CTHRC1) and the c‑Jun N‑terminal kinase (JNK) 1/2 inhibitor SP600125 on rat chondrocytes cultured in vitro with interleukin (IL)‑1β. Chondrocytes were treated with different doses of IL‑1β and cell viability and CTHRC1 expression were assessed using Cell Counting Kit‑8 and western blot assays, respectively. In separate experiments, chondrocytes were treated with CTHRC1‑expressing constructs (pLVX‑Puro‑CTHRC1) and/or SP600125, or IL‑1β with either CTHRC1 short hairpin (sh)RNA constructs (shNRA‑CTHRC1) or SP600125. The expression of CTHRC1, B‑cell lymphoma (Bcl)‑2, Bcl‑2‑associated X protein (Bax), cleaved caspase‑3, poly ADP ribose polymerase (PARP)‑1 and matrix metalloproteinase (MMP)‑13 was measured using reverse transcription‑quantitative polymerase chain reaction and western blotting assays. A Cell Counting Kit‑8 assay was performed to examine cell viability. Annexin V/propidium iodide staining and flow cytometry assays were used to detect chondrocyte apoptosis. The expression of JNK1/2 and phosphorylated JNK1/2 was measured using western blotting. CTHRC1 was highly expressed in patients with OA compared with normal controls. IL‑1β treatment (5, 10 and 20 ng/ml) increased the protein expression of CTHRC1 in a dose‑dependent manner and decreased the viability of chondrocytes in a time‑dependent manner. pLVX‑Puro‑CTHRC1 mimics the effect of IL‑1β on chondrocyte apoptosis and JNK1/2 activity, and this is reversed by SP600125 treatment. However, transfection with shRNA‑CTHRC1 or treatment with SP600125 inhibited IL‑1β‑induced cell apoptosis and JNK1/2 activation. These results indicate that CTHRC1 downregulation may protect chondrocytes from IL‑1β‑induced apoptosis by inactivating the JNK1/2 pathway.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

View References

1 

Vos T, Flaxman AD, Naghavi M, Lozano R, Michaud C, Ezzati M, Shibuya K, Salomon JA, Abdalla S, Aboyans V, et al: Years lived with disability (YLDs) for 1160 sequelae of 289 diseases and injuries 1990-2010: A systematic analysis for the Global Burden of Disease Study 2010. Lancet. 380:2163–2196. 2012. View Article : Google Scholar : PubMed/NCBI

2 

Karsdal MA, Michaelis M, Ladel C, Siebuhr AS, Bihlet AR, Andersen JR, Guehring H, Christiansen C, Bay-Jensen AC and Kraus VB: Disease-modifying treatments for osteoarthritis (DMOADs) of the knee and hip: Lessons learned from failures and opportunities for the future. Osteoarthritis Cartilage. 24:2013–2021. 2016. View Article : Google Scholar : PubMed/NCBI

3 

Findlay DM and Atkins GJ: Osteoblast-chondrocyte interactionsin osteoarthritis. Curr Osteoporos Rep. 12:127–134. 2014. View Article : Google Scholar : PubMed/NCBI

4 

Gore M, Tai KS, Sadosky A, Leslie D and Stacey BR: Clinical comorbidities, treatment patterns, and direct medical costs of patients with osteoarthritis inusual care: A retrospective claims database analysis. J Med Econ. 14:497–507. 2011. View Article : Google Scholar

5 

Portal-Núñez S, Esbrit P, Alcaraz MJ and Largo R: Oxidative stress, autophagy, epigenetic changes and regulation by miRNAs as potential therapeutic targets in osteoarthritis. Biochem Pharmacol. 108:1–10. 2016. View Article : Google Scholar

6 

Goldring MB and Goldring SR: Osteoarthritis. J Cell Physiol. 213:626–634. 2007. View Article : Google Scholar : PubMed/NCBI

7 

Charlier E, Relic B, Deroyer C, Malaise O, Neuville S, Collée J, Malaise MG and De Seny D: Insights on molecular mechanisms of chondrocytes death in osteoarthritis. Int J Mol Sci. 17:E21462016. View Article : Google Scholar : PubMed/NCBI

8 

Yan S, Wang M, Zhao J, Zhang H, Zhou C, Jin L, Zhang Y, Qiu X, Ma B and Fan Q: MicroRNA-34a affects chondrocyte apoptosis and proliferation by targeting the SIRT1/p53 signaling pathway during the pathogenesis of osteoarthritis. Int J Mol Med. 38:201–209. 2016. View Article : Google Scholar : PubMed/NCBI

9 

Xu XX, Zhang XH, Diao Y and Huang YX: Achyranthes bidentate saponins protect rat articular chondrocytes against interleukin-1β-induced inflammation and apoptosis in Svitro. Kaohsiung J Med Sci. 33:62–68. 2017. View Article : Google Scholar : PubMed/NCBI

10 

Wuelling M and Vortkamp A: Chondrocyte proliferation and differentiation. Endocr Dev. 21:1–11. 2011. View Article : Google Scholar : PubMed/NCBI

11 

Shakibaei M, Allaway D, Nebrich S and Mobasheri A: Botanical extracts from Rosehip (Rosa canina), Willow Bark (Salix alba), and Nettle Leaf (Urtica dioica) suppress IL-1β-induced NF-κB activation in canine articular chondrocytes. Evid Based Complement Alternat Med. 2012:5093832012. View Article : Google Scholar

12 

Kong D, Zheng T, Zhang M, Wang D, Du S, Li X, Fang J and Cao X: Static mechanical stress induces apoptosis in rat end plate chondrocytes through MAPK and mitochondria-dependent caspase activation signaling pathways. PLoS One. 8:e694032013. View Article : Google Scholar

13 

Boileau C, Pelletier JP, Tardif G, Fahmi H, Laufer S, Lavigne M and Martel-Pelletier J: The regulation of human MMP-13 by licofelone, an inhibitor of cyclooxygenases and5-lipoxygenase, in human osteoarthritic chondrocytes is mediated by the inhibition of the p38MAP kinase signalling pathway. Ann Rheum Dis. 64:891–898. 2005. View Article : Google Scholar

14 

Aigner T, McKenna L, Zien A, Fan Z, Gebhard PM and Zimmer R: Gene expression profiling of serum-and interleukin-1beta-stimulated primary human adult articular chondrocytes-a molecular analysis based on chondrocytes isolated from one donor. Cytokine. 31:227–240. 2005. View Article : Google Scholar : PubMed/NCBI

15 

Rao ZT, Wang SQ and Wang JQ: Exploring the osteoarthritis-related genes by gene expression analysis. Eur Rev Med Pharmacol Sci. 18:3056–3062. 2014.PubMed/NCBI

16 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2−ΔΔCT method. Methods. 25:402–408. 2001. View Article : Google Scholar

17 

Yasuda H, Oh CD, Chen D, de Crombrugghe B and Kim JH: A novel regulatory mechanism of type II collagen expression via a SOX9-dependent enhancer in intron 6. J Biol Chem. 292:528–538. 2017. View Article : Google Scholar :

18 

Aigner T, Soeder S and Haag J: IL-1beta and BMPs-interactive players of cartilage matrix degradation and regeneration. Eur Cell Mater. 12:49–56. 2006. View Article : Google Scholar

19 

Sellam J and Berenbaum F: Osteoarthritis and obesity. Rev Prat. 62:621–624. 2012.In French. PubMed/NCBI

20 

Durmus T, LeClair RJ, Park KS, Terzic A, Yoon JK and Lindner V: Expression analysis of the novel gene collagen triple helix repeat containing-1 (Cthrc1). Gene Expr Patterns. 6:935–940. 2006. View Article : Google Scholar : PubMed/NCBI

21 

Kimura H, Kwan KM, Zhang Z, Deng JM, Darnay BG, Behringer RR, Nakamura T, de Crombrugghe B and Akiyama H: Cthrc1 is a positive regulator of osteoblastic bone formation. PLoS One. 3:e31742008. View Article : Google Scholar : PubMed/NCBI

22 

Kudryavtseva E, Forde TS, Pucker AD and Adarichev VA: Wnt signaling genes of murine chromosome 15 are involved in sex-affected pathways of inflammatory arthritis. Arthritis Rheum. 64:1057–1068. 2012. View Article : Google Scholar

23 

Lawrence T: The nuclear factor NF-kappaB pathway in inflammation. Cold Spring Harb Perspect Biol. 1:a0016512009. View Article : Google Scholar

24 

Kapoor M, Martelpelletier J, Lajeunesse D, Pelletier JP and Fahmi H: Role of proinflammatory cytokines in the pathophysiology of osteoarthritis. Nat Rev Rheumatol. 7:33–42. 2011. View Article : Google Scholar

25 

Bian Q, Wang YJ, Liu SF and Li YP: Osteoarthritis: Genetic factors, animal models, mechanisms, and therapies. Front Biosci. 4:74–100. 2012. View Article : Google Scholar

26 

Jotanovic Z, Mihelic R, Sestan B and Dembic Z: Role of interleukin-1 inhibitors in osteoarthritis: An evidence-based review. Drugs Aging. 29:343–358. 2012. View Article : Google Scholar : PubMed/NCBI

27 

Fernandes JC, Martel-Pelletier J and Pelletier JP: The role of cytokines in osteoarthritis pathophysiology. Biorheology. 39:237–246. 2002.PubMed/NCBI

28 

Karaliotas GI, Mavridis K, Scorilas A and Babis GC: Quantitative analysis of the Mrna expression levels of BCL2 and BAX genes in human osteoarthritis and normal articular cartilage: An investigation into their differential expression. Mol Med Rep. 12:4514–4521. 2015. View Article : Google Scholar : PubMed/NCBI

29 

Cheng W, Wu D, Zuo Q, Wang Z and Fan W: Ginsenoside Rb1 prevents interleukin-1beta induced inflammation and apoptosis in human articular chondrocytes. Int Orthop. 37:2065–2070. 2013. View Article : Google Scholar : PubMed/NCBI

30 

Yang D, Guo S, Zhang T and Li H: Hypothermia attenuatesis chemia/reperfusion-induced endothelial cell apoptosis via alterations in apoptotic pathways and JNK signaling. FEBS Lett. 583:2500–2506. 2009. View Article : Google Scholar : PubMed/NCBI

31 

Fan Z, Söder S, Oehler S, Fundel K and Aigner T: Activation of interleukin-1 signaling cascades in normal and osteoarthritic articular cartilage. Am J Pathol. 171:938–946. 2007. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Zhang Q, Yin ZS, Zhang FW, Cao K and Sun HY: CTHRC1 mediates IL‑1β‑induced apoptosis in chondrocytes via JNK1/2 signaling. Int J Mol Med 41: 2270-2278, 2018.
APA
Zhang, Q., Yin, Z., Zhang, F., Cao, K., & Sun, H. (2018). CTHRC1 mediates IL‑1β‑induced apoptosis in chondrocytes via JNK1/2 signaling. International Journal of Molecular Medicine, 41, 2270-2278. https://doi.org/10.3892/ijmm.2018.3403
MLA
Zhang, Q., Yin, Z., Zhang, F., Cao, K., Sun, H."CTHRC1 mediates IL‑1β‑induced apoptosis in chondrocytes via JNK1/2 signaling". International Journal of Molecular Medicine 41.4 (2018): 2270-2278.
Chicago
Zhang, Q., Yin, Z., Zhang, F., Cao, K., Sun, H."CTHRC1 mediates IL‑1β‑induced apoptosis in chondrocytes via JNK1/2 signaling". International Journal of Molecular Medicine 41, no. 4 (2018): 2270-2278. https://doi.org/10.3892/ijmm.2018.3403
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang Q, Yin ZS, Zhang FW, Cao K and Sun HY: CTHRC1 mediates IL‑1β‑induced apoptosis in chondrocytes via JNK1/2 signaling. Int J Mol Med 41: 2270-2278, 2018.
APA
Zhang, Q., Yin, Z., Zhang, F., Cao, K., & Sun, H. (2018). CTHRC1 mediates IL‑1β‑induced apoptosis in chondrocytes via JNK1/2 signaling. International Journal of Molecular Medicine, 41, 2270-2278. https://doi.org/10.3892/ijmm.2018.3403
MLA
Zhang, Q., Yin, Z., Zhang, F., Cao, K., Sun, H."CTHRC1 mediates IL‑1β‑induced apoptosis in chondrocytes via JNK1/2 signaling". International Journal of Molecular Medicine 41.4 (2018): 2270-2278.
Chicago
Zhang, Q., Yin, Z., Zhang, F., Cao, K., Sun, H."CTHRC1 mediates IL‑1β‑induced apoptosis in chondrocytes via JNK1/2 signaling". International Journal of Molecular Medicine 41, no. 4 (2018): 2270-2278. https://doi.org/10.3892/ijmm.2018.3403
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team