Open Access

Structural characterization, immune regulation and antioxidant activity of a new heteropolysaccharide from Cantharellus cibarius Fr.

  • Authors:
    • Daqun Zhao
    • Xiang Ding
    • Yiling Hou
    • Wanru Hou
    • Lu Liu
    • Ting Xu
    • Danni Yang
  • View Affiliations

  • Published online on: February 1, 2018     https://doi.org/10.3892/ijmm.2018.3450
  • Pages: 2744-2754
  • Copyright: © Zhao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

A new heteropolysaccharide was extracted and purified from the fruiting bodies of Cantharellus cibarius Fr. The Cantharellus cibarius Fr. polysaccharide (CC-1) had a molecular weight of 61,056 kDa and was mainly formed of the glucose and xylose at ratio of 5:1. Structure identification of CC-1 was analysed by a combined application of total hydrolysis, high performance liquid chromatography (HPLC), methylation analysis, gas chromatography-mass spectrometry (GC-MS), infrared (IR) spectra and nuclear magnetic resonance (NMR) spectroscopy. The experimental results showed that CC-1 had a backbone of 1,4-linked-β-D-glucose which branched at O-6 and the branches were mainly composed of 6→1)-α-D-xylopyranose residue. CC-1 exhibited significant in vitro antioxidant effect and proliferation effect of immune cells. The activity study showed CC-1 has ability to clear the ABTS+ free radical and DPPH- free radical in a certain range of concentration. The proliferation activity of the immune cells showed that the proliferation effect on B cells was very significant (P<0.001) in the concentration of 0.625-80 mg/ml; and the effect of T cell proliferation was also very significant (P<0.001) in the concentration of 5-20 mg/ml. The result of this study introduced Cantharellus cibarius Fr. as a possible valuable source in exhibiting unique immunoregulatory and antioxidant properties.
View Figures
View References

Related Articles

Journal Cover

May-2018
Volume 41 Issue 5

Print ISSN: 1107-3756
Online ISSN:1791-244X

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Zhao D, Ding X, Hou Y, Hou W, Liu L, Xu T and Yang D: Structural characterization, immune regulation and antioxidant activity of a new heteropolysaccharide from Cantharellus cibarius Fr.. Int J Mol Med 41: 2744-2754, 2018
APA
Zhao, D., Ding, X., Hou, Y., Hou, W., Liu, L., Xu, T., & Yang, D. (2018). Structural characterization, immune regulation and antioxidant activity of a new heteropolysaccharide from Cantharellus cibarius Fr.. International Journal of Molecular Medicine, 41, 2744-2754. https://doi.org/10.3892/ijmm.2018.3450
MLA
Zhao, D., Ding, X., Hou, Y., Hou, W., Liu, L., Xu, T., Yang, D."Structural characterization, immune regulation and antioxidant activity of a new heteropolysaccharide from Cantharellus cibarius Fr.". International Journal of Molecular Medicine 41.5 (2018): 2744-2754.
Chicago
Zhao, D., Ding, X., Hou, Y., Hou, W., Liu, L., Xu, T., Yang, D."Structural characterization, immune regulation and antioxidant activity of a new heteropolysaccharide from Cantharellus cibarius Fr.". International Journal of Molecular Medicine 41, no. 5 (2018): 2744-2754. https://doi.org/10.3892/ijmm.2018.3450