Open Access

Amelioration of inflammatory responses by Socheongryong-Tang, a traditional herbal medicine, in RAW 264.7 cells and rats

  • Authors:
    • Sang Mi Park
    • Tae Hoon Lee
    • Rongjie Zhao
    • Youn Sook Kim
    • Ji Yun Jung
    • Chung A. Park
    • Kyung Hwan Jegal
    • Sae Kwang Ku
    • Jae Kwang Kim
    • Chul Won Lee
    • Young Woo Kim
    • Il Je Cho
    • Won G. An
    • Sang Chan Kim
  • View Affiliations

  • Published online on: February 6, 2018     https://doi.org/10.3892/ijmm.2018.3465
  • Pages: 2771-2783
  • Copyright: © Park et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Socheongryong-Tang (SCRT) is a natural medicine prescription that has been mainly used in East Asia for the treatment of inflammatory disorders, including asthma and allergic rhinitis. The present study evaluated the anti-inflammatory effects of SCRT on lipopolysaccharide (LPS)-stimulated RAW 264.7 cells and in a rat model of carrageenan (CA)-induced paw edema. Levels of tumor necrosis factor-α (TNF-α), interleukin (IL)-1β, IL-6 and prostaglandin E2 (PGE2) in the culture supernatant were quantified and nitric oxide (NO) production was monitored. In addition, the effect of SCRT on the protein expression of nuclear factor-κB (NF-κB), mitogen-activated protein kinases (MAPKs), inducible NO synthase (iNOS) and cyclooxygenase-2 (COX-2) was assessed by western blot analysis. Furthermore, the effects of SCRT on acute inflammation in vivo and changes in the histomorphometry and histopathology of paw skin were observed using CA-treated rats. SCRT (1 mg/ml) inhibited the LPS-induced changes in the protein expression of NF-κB, JNK, ERK1/2, iNOS and COX-2, as well as the production of NO, PGE2 and cytokines. In the rat paw edema assay, administration of 1 g/kg of lyophilized powder obtained from the aqueous extracts of SCRT for 3 consecutive days inhibited the CA-induced increases in skin thickness, mast cell degranulation, and infiltration of inflammatory cells in the ventral and dorsal pedis skin within 4 h. These results demonstrated that SCRT exerts its anti-inflammatory activities in LPS-stimulated RAW 264.7 cells through decreasing the production of inflammatory mediators, including PGE2, NO and cytokines, via suppression of the NF-κB and JNK and ERK1/2 signaling pathways. In addition, the data of the CA-induced paw edema indicated an anti-edema effect of SCRT. SCRT (1 g/kg) reduced acute edematous inflammation through inhibition of mast cell degranulation and infiltration of inflammatory cells. Therefore, the present study provided scientific evidence for the anti-inflammatory activities of SCRT as well as the underlying mechanisms.
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May-2018
Volume 41 Issue 5

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Park SM, Lee TH, Zhao R, Kim YS, Jung JY, Park CA, Jegal KH, Ku SK, Kim JK, Lee CW, Lee CW, et al: Amelioration of inflammatory responses by Socheongryong-Tang, a traditional herbal medicine, in RAW 264.7 cells and rats. Int J Mol Med 41: 2771-2783, 2018
APA
Park, S.M., Lee, T.H., Zhao, R., Kim, Y.S., Jung, J.Y., Park, C.A. ... Kim, S.C. (2018). Amelioration of inflammatory responses by Socheongryong-Tang, a traditional herbal medicine, in RAW 264.7 cells and rats. International Journal of Molecular Medicine, 41, 2771-2783. https://doi.org/10.3892/ijmm.2018.3465
MLA
Park, S. M., Lee, T. H., Zhao, R., Kim, Y. S., Jung, J. Y., Park, C. A., Jegal, K. H., Ku, S. K., Kim, J. K., Lee, C. W., Kim, Y. W., Cho, I. J., An, W. G., Kim, S. C."Amelioration of inflammatory responses by Socheongryong-Tang, a traditional herbal medicine, in RAW 264.7 cells and rats". International Journal of Molecular Medicine 41.5 (2018): 2771-2783.
Chicago
Park, S. M., Lee, T. H., Zhao, R., Kim, Y. S., Jung, J. Y., Park, C. A., Jegal, K. H., Ku, S. K., Kim, J. K., Lee, C. W., Kim, Y. W., Cho, I. J., An, W. G., Kim, S. C."Amelioration of inflammatory responses by Socheongryong-Tang, a traditional herbal medicine, in RAW 264.7 cells and rats". International Journal of Molecular Medicine 41, no. 5 (2018): 2771-2783. https://doi.org/10.3892/ijmm.2018.3465