Open Access

Experimental study on the neurotoxic effect of β-amyloid on the cytoskeleton of PC12 cells

  • Authors:
    • Lai Wang
    • Jingjing Cao
    • Zhenyu Shi
    • Wenjuan Fan
    • Hongliang Liu
    • Jinbo Deng
    • Jiexin Deng
  • View Affiliations

  • Published online on: February 7, 2018     https://doi.org/10.3892/ijmm.2018.3467
  • Pages: 2764-2770
  • Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The aim of the present study was to establish a cell model of Alzheimer's disease (AD) and investigate the neurotoxic effects of β-amyloid (Aβ) on the cytoskeleton. PC12 cells were cultured and treated with Aβ25‑35, and cell survival was analyzed with the MTT assay. Cell apoptosis was visualized using 4',6-diamidino-2-phenylindole staining and the terminal deoxynucleotidyl transferase dUTP nick-end labeling assay. Immunocytochemistry and phalloidin staining were used to label the cytoskeleton of PC12 cells. Aβ25-35 was found to induce PC12 cell apoptosis in a dose-dependent manner (P<0.05). Moreover, Aβ25-35 also caused dose-dependent disintegration of the cytoskeleton (P<0.05). Therefore, the PC12 cell cytoskeleton was found to be sensitive to Aβ25-35 neurotoxicity. The disintegration of the cytoskeleton is likely an important pathological alteration in AD, and Aβ is a key molecule involved in AD pathogenesis.
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May-2018
Volume 41 Issue 5

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Wang L, Cao J, Shi Z, Fan W, Liu H, Deng J and Deng J: Experimental study on the neurotoxic effect of β-amyloid on the cytoskeleton of PC12 cells. Int J Mol Med 41: 2764-2770, 2018
APA
Wang, L., Cao, J., Shi, Z., Fan, W., Liu, H., Deng, J., & Deng, J. (2018). Experimental study on the neurotoxic effect of β-amyloid on the cytoskeleton of PC12 cells. International Journal of Molecular Medicine, 41, 2764-2770. https://doi.org/10.3892/ijmm.2018.3467
MLA
Wang, L., Cao, J., Shi, Z., Fan, W., Liu, H., Deng, J., Deng, J."Experimental study on the neurotoxic effect of β-amyloid on the cytoskeleton of PC12 cells". International Journal of Molecular Medicine 41.5 (2018): 2764-2770.
Chicago
Wang, L., Cao, J., Shi, Z., Fan, W., Liu, H., Deng, J., Deng, J."Experimental study on the neurotoxic effect of β-amyloid on the cytoskeleton of PC12 cells". International Journal of Molecular Medicine 41, no. 5 (2018): 2764-2770. https://doi.org/10.3892/ijmm.2018.3467