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Article

The expression and activation of sex steroid receptors in the preeclamptic placenta

  • Authors:
    • Mee‑Na Park
    • Kyung‑Hee Park
    • Jae‑Eon Lee
    • Ye Young Shin
    • Sung‑Min An
    • Seong Soo Kang
    • Wan‑Seob Cho
    • Beum‑Soo An
    • Seung Chul Kim
  • View Affiliations / Copyright

    Affiliations: Department of Biomaterials Science, College of Natural Resources & Life Science/Life and Industry Convergence Research Institute, Pusan National University, Miryang, Gyeongsangnam‑do 627‑706, Republic of Korea, Department of Pediatrics, Biomedical Research Institute, Pusan National University School of Medicine, Busan 302‑739, Republic of Korea, Department of Veterinary Surgery, College of Veterinary Medicine, Chonnam National University, Gwangju 500‑757, Republic of Korea, Department of Medical Biotechnology, School of Natural Resources and Life Science, Dong‑A University, Busan 604‑714, Republic of Korea, Department of Obstetrics and Gynecology, Biomedical Research Institute, Pusan National University School of Medicine, Busan 302‑739, Republic of Korea
  • Pages: 2943-2951
    |
    Published online on: February 8, 2018
       https://doi.org/10.3892/ijmm.2018.3474
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Abstract

Estrogen and progesterone are the main pregnancy hormones produced by the placenta. It is well understood that estrogen stimulates angiogenesis in the uterus during the reproductive cycle. Although the estrogen and progesterone signaling pathways are assumed to be associated with placental vascularization and preeclampsia, expression of estrogen receptors (ESRs) and progesterone receptor (PGR) in the placenta have not been well studied. The present study examined the expression patterns of steroid hormone receptors in placentas. Human placenta samples were collected and divided into normal and preeclampsia groups. Results revealed that expression levels of ESR1 were reduced, whereas ESR2 and PGR were elevated in preeclamptic placentas. To generate an in vitro preeclampsia environment, human placenta‑derived BeWo cells were incubated under hypoxic conditions, or treated with catechol‑O‑methyl transferase inhibitor (COMT‑in) or L‑NG‑nitroarginine methyl ester (L‑NAME). Expression levels of ESR1, ESR2 and PGR in hypoxic cells demonstrated similar regulation as those in placentas from women with preeclampsia. Although COMT‑in and L‑NAME did not significantly regulate the expression levels of the receptors, COMT‑in translocated ESR2 and PGR from the nucleus to the cytoplasm, indicating that these receptors were inactivated. These results suggested that ESRs and PGR are associated with symptoms of preeclampsia in the placenta. The expression of ESR1 was reduced in preeclamptic placenta and hypoxic BeWo cells. In addition, the activation of ESR2 and PGR was blocked in placenta cells subjected to COMT‑in treatment. The reduced ESR1 expression and inactivation of ESR2 and PGR proteins may affect the physiological complications of preeclampsia in the placenta.
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Copy and paste a formatted citation
Spandidos Publications style
Park MN, Park KH, Lee JE, Shin YY, An SM, Kang SS, Cho WS, An BS and Kim SC: The expression and activation of sex steroid receptors in the preeclamptic placenta. Int J Mol Med 41: 2943-2951, 2018.
APA
Park, M., Park, K., Lee, J., Shin, Y.Y., An, S., Kang, S.S. ... Kim, S.C. (2018). The expression and activation of sex steroid receptors in the preeclamptic placenta. International Journal of Molecular Medicine, 41, 2943-2951. https://doi.org/10.3892/ijmm.2018.3474
MLA
Park, M., Park, K., Lee, J., Shin, Y. Y., An, S., Kang, S. S., Cho, W., An, B., Kim, S. C."The expression and activation of sex steroid receptors in the preeclamptic placenta". International Journal of Molecular Medicine 41.5 (2018): 2943-2951.
Chicago
Park, M., Park, K., Lee, J., Shin, Y. Y., An, S., Kang, S. S., Cho, W., An, B., Kim, S. C."The expression and activation of sex steroid receptors in the preeclamptic placenta". International Journal of Molecular Medicine 41, no. 5 (2018): 2943-2951. https://doi.org/10.3892/ijmm.2018.3474
Copy and paste a formatted citation
x
Spandidos Publications style
Park MN, Park KH, Lee JE, Shin YY, An SM, Kang SS, Cho WS, An BS and Kim SC: The expression and activation of sex steroid receptors in the preeclamptic placenta. Int J Mol Med 41: 2943-2951, 2018.
APA
Park, M., Park, K., Lee, J., Shin, Y.Y., An, S., Kang, S.S. ... Kim, S.C. (2018). The expression and activation of sex steroid receptors in the preeclamptic placenta. International Journal of Molecular Medicine, 41, 2943-2951. https://doi.org/10.3892/ijmm.2018.3474
MLA
Park, M., Park, K., Lee, J., Shin, Y. Y., An, S., Kang, S. S., Cho, W., An, B., Kim, S. C."The expression and activation of sex steroid receptors in the preeclamptic placenta". International Journal of Molecular Medicine 41.5 (2018): 2943-2951.
Chicago
Park, M., Park, K., Lee, J., Shin, Y. Y., An, S., Kang, S. S., Cho, W., An, B., Kim, S. C."The expression and activation of sex steroid receptors in the preeclamptic placenta". International Journal of Molecular Medicine 41, no. 5 (2018): 2943-2951. https://doi.org/10.3892/ijmm.2018.3474
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