Metabolic changes associated with papillary thyroid carcinoma: A nuclear magnetic resonance-based metabolomics study

  • Authors:
    • Yanyun Li
    • Minjian Chen
    • Cuiping Liu
    • Yankai Xia
    • Bo Xu
    • Yanhui Hu
    • Ting Chen
    • Meiping Shen
    • Wei Tang
  • View Affiliations

  • Published online on: February 16, 2018     https://doi.org/10.3892/ijmm.2018.3494
  • Pages: 3006-3014
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Abstract

Papillary thyroid carcinoma (PTC) is the most common thyroid cancer. Nuclear magnetic resonance (NMR)‑based metabolomic technique is the gold standard in metabolite structural elucidation, and can provide different coverage of information compared with other metabolomic techniques. Here, we firstly conducted NMR based metabolomics study regarding detailed metabolic changes especially metabolic pathway changes related to PTC pathogenesis. 1H NMR-based metabolomic technique was adopted in conju­nction with multivariate analysis to analyze matched tumor and normal thyroid tissues obtained from 16 patients. The results were further annotated with Kyoto Encyclopedia of Genes and Genomes (KEGG), and Human Metabolome Database, and then were analyzed using modules of pathway analysis and enrichment analysis of MetaboAnalyst 3.0. Based on the analytical techniques, we established the models of principal component analysis (PCA), partial least squares-discriminant analysis (PLS-DA), and orthogonal partial least-squares discriminant analysis (OPLS‑DA) which could discriminate PTC from normal thyroid tissue, and found 15 robust differentiated metabolites from two OPLS-DA models. We identified 8 KEGG pathways and 3 pathways of small molecular pathway database which were significantly related to PTC by using pathway analysis and enrichment analysis, respectively, through which we identified metabolisms related to PTC including branched chain amino acid metabolism (leucine and valine), other amino acid metabolism (glycine and taurine), glycolysis (lactate), tricarboxylic acid cycle (citrate), choline metabolism (choline, ethanolamine and glycerolphosphocholine) and lipid metabolism (very-low‑density lipoprotein and low-density lipoprotein). In conclusion, the PTC was characterized with increased glycolysis and inhibited tricarboxylic acid cycle, increased oncogenic amino acids as well as abnormal choline and lipid metabolism. The findings in this study provide new insights into detailed metabolic changes of PTC, and hold great potential in the treatment of PTC.
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May-2018
Volume 41 Issue 5

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Li Y, Chen M, Liu C, Xia Y, Xu B, Hu Y, Chen T, Shen M and Tang W: Metabolic changes associated with papillary thyroid carcinoma: A nuclear magnetic resonance-based metabolomics study. Int J Mol Med 41: 3006-3014, 2018
APA
Li, Y., Chen, M., Liu, C., Xia, Y., Xu, B., Hu, Y. ... Tang, W. (2018). Metabolic changes associated with papillary thyroid carcinoma: A nuclear magnetic resonance-based metabolomics study. International Journal of Molecular Medicine, 41, 3006-3014. https://doi.org/10.3892/ijmm.2018.3494
MLA
Li, Y., Chen, M., Liu, C., Xia, Y., Xu, B., Hu, Y., Chen, T., Shen, M., Tang, W."Metabolic changes associated with papillary thyroid carcinoma: A nuclear magnetic resonance-based metabolomics study". International Journal of Molecular Medicine 41.5 (2018): 3006-3014.
Chicago
Li, Y., Chen, M., Liu, C., Xia, Y., Xu, B., Hu, Y., Chen, T., Shen, M., Tang, W."Metabolic changes associated with papillary thyroid carcinoma: A nuclear magnetic resonance-based metabolomics study". International Journal of Molecular Medicine 41, no. 5 (2018): 3006-3014. https://doi.org/10.3892/ijmm.2018.3494