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Effect of lithocholic acid on biologically active α,β-unsaturated aldehydes induced by H2O2 in glioma mitochondria for use in glioma treatment

  • Authors:
    • Dan Wang
    • Li Bie
    • Yanbin Su
    • Haoran Xu
    • Fengrong Zhang
    • Yanwen Su
    • Bo Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Ophthalmology, First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China, Department of Neurosurgery, First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China, Key Laboratory for Molecular Enzymology and Engineering, Jilin University, Changchun, Jilin 130021, P.R. China, Jilin Institute for Food and Drug Control, Changchun, Jilin 130033, P.R. China, College of Chemical and Pharmaceutical Engineering, Jilin Institute of Chemical Technology, Changchun, Jilin 132022, P.R. China
    Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 3195-3202
    |
    Published online on: March 2, 2018
       https://doi.org/10.3892/ijmm.2018.3530
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Abstract

Lithocholic acid (LCA) is known to kill glioma cells while sparing normal neuronal cells. However, the anti-glioma mechanism of LCA is unclear at present. Although malondialdehyde (MDA) is not specific to detect tumors, biologically active α,β-unsaturated aldehydes can be used to detect the outcome of gliomas, especially the mitochondria, as a research tool. The purpose of this research was to determine the optimum conditions for a lipid peroxidation model, according to changes in the aldehydes formed from the reaction between 2-thiobarbituric acid and biologically active α,β-unsaturated aldehydes. Experimental methods and procedures were successfully established for a model of lipid peroxidation induced by H2O2 in glioma mitochondria for glioma treatment and optimum conditions for LCA treatment were determined. The optimal conditions for the model were a glioma mitochondrial concentration of 1.5 mg/ml, H2O2 concentration of 0.3 mg/ml, duration of action of 30 min, and addition of 4.0 ml of 46 mM thiobarbituric acid. The effect of LCA, as determined by changes in the UV peaks at 450, 495, and 532 nm, was optimal at a concentration of 100 µM, a duration of action of 15 min, and in an acidic microenvironment. The study concluded that a suitable concentration of LCA has anti-glioma effects as determined by the effect on changes in the UV peaks at 450, 495 and 532 nm and the mitochondrial model developed should be conducive to further in-depth research.
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Copy and paste a formatted citation
Spandidos Publications style
Wang D, Bie L, Su Y, Xu H, Zhang F, Su Y and Zhang B: Effect of lithocholic acid on biologically active α,β-unsaturated aldehydes induced by H2O2 in glioma mitochondria for use in glioma treatment. Int J Mol Med 41: 3195-3202, 2018.
APA
Wang, D., Bie, L., Su, Y., Xu, H., Zhang, F., Su, Y., & Zhang, B. (2018). Effect of lithocholic acid on biologically active α,β-unsaturated aldehydes induced by H2O2 in glioma mitochondria for use in glioma treatment. International Journal of Molecular Medicine, 41, 3195-3202. https://doi.org/10.3892/ijmm.2018.3530
MLA
Wang, D., Bie, L., Su, Y., Xu, H., Zhang, F., Su, Y., Zhang, B."Effect of lithocholic acid on biologically active α,β-unsaturated aldehydes induced by H2O2 in glioma mitochondria for use in glioma treatment". International Journal of Molecular Medicine 41.6 (2018): 3195-3202.
Chicago
Wang, D., Bie, L., Su, Y., Xu, H., Zhang, F., Su, Y., Zhang, B."Effect of lithocholic acid on biologically active α,β-unsaturated aldehydes induced by H2O2 in glioma mitochondria for use in glioma treatment". International Journal of Molecular Medicine 41, no. 6 (2018): 3195-3202. https://doi.org/10.3892/ijmm.2018.3530
Copy and paste a formatted citation
x
Spandidos Publications style
Wang D, Bie L, Su Y, Xu H, Zhang F, Su Y and Zhang B: Effect of lithocholic acid on biologically active α,β-unsaturated aldehydes induced by H2O2 in glioma mitochondria for use in glioma treatment. Int J Mol Med 41: 3195-3202, 2018.
APA
Wang, D., Bie, L., Su, Y., Xu, H., Zhang, F., Su, Y., & Zhang, B. (2018). Effect of lithocholic acid on biologically active α,β-unsaturated aldehydes induced by H2O2 in glioma mitochondria for use in glioma treatment. International Journal of Molecular Medicine, 41, 3195-3202. https://doi.org/10.3892/ijmm.2018.3530
MLA
Wang, D., Bie, L., Su, Y., Xu, H., Zhang, F., Su, Y., Zhang, B."Effect of lithocholic acid on biologically active α,β-unsaturated aldehydes induced by H2O2 in glioma mitochondria for use in glioma treatment". International Journal of Molecular Medicine 41.6 (2018): 3195-3202.
Chicago
Wang, D., Bie, L., Su, Y., Xu, H., Zhang, F., Su, Y., Zhang, B."Effect of lithocholic acid on biologically active α,β-unsaturated aldehydes induced by H2O2 in glioma mitochondria for use in glioma treatment". International Journal of Molecular Medicine 41, no. 6 (2018): 3195-3202. https://doi.org/10.3892/ijmm.2018.3530
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