Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
International Journal of Molecular Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1107-3756 Online ISSN: 1791-244X
Journal Cover
June-2018 Volume 41 Issue 6

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
June-2018 Volume 41 Issue 6

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

Valproic acid prevents glucocorticoid‑induced osteonecrosis of the femoral head of rats

  • Authors:
    • Ding Zhou
    • Yi‑Xuan Chen
    • Jun‑Hui Yin
    • Shi‑Cong Tao
    • Shang‑Chun Guo
    • Zhan‑Ying Wei
    • Yong Feng
    • Chang‑Qing Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Orthopaedic Surgery, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai 200233, P.R. China, Institute of Microsurgery on Extremities, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai 200233, P.R. China, Metabolic Bone Disease and Genetic Research Unit, Division of Osteoporosis and Bone Disease, Department of Endocrinology and Metabolism, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai 200233, P.R. China
    Copyright: © Zhou et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 3433-3447
    |
    Published online on: March 6, 2018
       https://doi.org/10.3892/ijmm.2018.3534
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Glucocorticoids (GCs) are the most common cause of atraumatic osteonecrosis of the femoral head (ONFH) because their effect compromises the osteogenic capability of bone marrow‑derived mesenchymal stem cells (BMSCs). Valproic acid (VPA) is a widely used anti‑epileptic and anti‑convulsant drug. Previous studies have reported that VPA promotes osteogenic differentiation of MSCs in vitro and osteogenesis in vivo as a histone deacetylase (HDAC) inhibitor. The purpose of the present study was to investigate the efficacy of VPA as a precautionary treatment of ONFH after GC treatment in rats. In vitro, the effect of VPA, dexamethasone or a combination treatment of the two on the proliferation and osteogenic differentiation of human BMSCs was assessed using a Cell Counting Kit‑8 and apoptosis assays, and by measuring the expression of proteins associated with osteogenesis. In vivo, a GC‑induced ONFH model was established in rats and VPA was added during GC treatment to investigate the preventive effect of VPA against ONFH. Rat BMSCs were also extracted to investigate the osteogenic capacity. The results of micro‑computed tomography scanning, angiography of the femoral head and histological and immunohistochemical analyses indicated that 11 of 15 rats induced with methylprednisolone (MP) presented with ONFH, while only 2 of 15 rats treated with a combination of MP and VPA developed ONFH. VPA produced beneficial effects on subchondral bone trabeculae in the femoral head with significant preservation of bone volume and blood supply, as well as improved osteogenic capability of BMSCs compared with those in rats treated with GC alone. In conclusion, VPA attenuated the inhibitory effect of GC on BMSC proliferation and osteogenesis by inhibiting apoptosis and elevating the expression of proteins associated with osteogenesis, which may contribute to the prevention of GC‑induced ONFH in rats.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

Figure 7

Figure 8

Figure 9

Figure 10

Figure 11

Figure 12

View References

1 

Assouline-Dayan Y, Chang C, Greenspan A, Shoenfeld Y and Gershwin ME: Pathogenesis and natural history of osteonecrosis. Semin Arthritis Rheum. 32:94–124. 2002. View Article : Google Scholar : PubMed/NCBI

2 

Mont MA, Jones LC and Hungerford DS: Nontraumatic osteonecrosis of the femoral head: Ten years later. J Bone Joint Surg Am. 88:1117–1132. 2006.PubMed/NCBI

3 

Tan G, Kang PD and Pei FX: Glucocorticoids affect the metabolism of bone marrow stromal cells and lead to osteonecrosis of the femoral head: A review. Chin Med J. 125:134–139. 2012.PubMed/NCBI

4 

Tait AS, Butts CL and Sternberg EM: The role of glucocorticoids and progestins in inflammatory, autoimmune, and infectious disease. J Leukoc Biol. 84:924–931. 2008. View Article : Google Scholar : PubMed/NCBI

5 

Kirwan JR: The effect of glucocorticoids on joint destruction in rheumatoid arthritis. The arthritis and rheumatism council Low-dose glucocorticoid study group. N Engl J Med. 333:142–146. 1995. View Article : Google Scholar : PubMed/NCBI

6 

Salmon SE, Crowley JJ, Grogan TM, Finley P, Pugh RP and Barlogie B: Combination chemotherapy, glucocorticoids, and interferon alfa in the treatment of multiple myeloma: A Southwest oncology group study. J Clin Oncol. 12:2405–2414. 1994. View Article : Google Scholar : PubMed/NCBI

7 

Hall ED and Braughler JM: Glucocorticoid mechanisms in acute spinal cord injury: A review and therapeutic rationale. Surg Neurol. 18:320–327. 1982. View Article : Google Scholar : PubMed/NCBI

8 

Mont MA, Zywiel MG, Marker DR, McGrath MS and Delanois RE: The natural history of untreated asymptomatic osteonecrosis of the femoral head: A systematic literature review. J Bone Joint Surg Am. 92:2165–2170. 2010. View Article : Google Scholar : PubMed/NCBI

9 

Boss JH: Experimental models of osteonecrosis of the femoral head. J Orthop Sci. 9:533–534. 2004. View Article : Google Scholar : PubMed/NCBI

10 

Lavernia CJ, Sierra RJ and Grieco FR: Osteonecrosis of the femoral head. J Am Acad Orthop Surg. 7:250–261. 1999. View Article : Google Scholar : PubMed/NCBI

11 

Kerachian MA, Cournoyer D, Harvey EJ, Chow TY, Bégin LR, Nahal A and Séguin C: New insights into the pathogenesis of glucocorticoid-induced avascular necrosis: Microarray analysis of gene expression in a rat model. Arthritis Res Ther. 12:R1242010. View Article : Google Scholar : PubMed/NCBI

12 

Cenni E, Fotia C, Rustemi E, Yuasa K, Caltavuturo G, Giunti A and Baldini N: Idiopathic and secondary osteonecrosis of the femoral head show different thrombophilic changes and normal or higher levels of platelet growth factors. Acta Orthop. 82:42–49. 2011. View Article : Google Scholar : PubMed/NCBI

13 

Hernigou P, Beaujean F and Lambotte JC: Decrease in the mesenchymal stem-cell pool in the proximal femur in corticosteroid-induced osteonecrosis. J Bone Joint Surg Br. 81:349–355. 1999. View Article : Google Scholar : PubMed/NCBI

14 

Hernigou P and Beaujean F: Abnormalities in the bone marrow of the iliac crest in patients who have osteonecrosis secondary to corticosteroid therapy or alcohol abuse. J Bone Joint Surg Am. 79:1047–1053. 1997. View Article : Google Scholar : PubMed/NCBI

15 

Jia Y, Chu TW and Zhou Y: Repair process in experimentally induced avascular necrosis of femoral head in rabbits. Chongqing Med. 2006.

16 

Takaoka K, Yoshioka T, Hosoya T, Ono K and Takase T: The repair process in experimentally induced avascular necrosis of the femoral head in dogs. Arch Orthop Trauma Surg. 99:109–115. 1981. View Article : Google Scholar : PubMed/NCBI

17 

Malizos KN, Quarles LD, Seaber AV, Rizk WS and Urbaniak JR: An experimental canine model of osteonecrosis: Characterization of the repair process. J Orthop Res. 11:350–357. 1993. View Article : Google Scholar : PubMed/NCBI

18 

Shapiro F, Connolly S, Zurakowski D, Menezes N, Olear E, Jimenez M, Flynn E and Jaramillo D: Femoral head deformation and repair following induction of ischemic necrosis. J Bone Joint Surg Am. 91:2903–2914. 2009. View Article : Google Scholar : PubMed/NCBI

19 

Koromila T, Baniwal SK, Song YS, Martin A, Xiong J and Frenkel B: Glucocorticoids antagonize RUNX2 during osteoblast differentiation in cultures of ST2 pluripotent mesenchymal cells. J Cell Biochem. 115:27–33. 2014. View Article : Google Scholar

20 

O'Brien CA, Jia D, Plotkin LI, Bellido T, Powers CC, Stewart SA, Manolagas SC and Weinstein RS: Glucocorticoids act directly on osteoblasts and osteocytes to induce their apoptosis and reduce bone formation and strength. Endocrinology. 145:1835–1841. 2004. View Article : Google Scholar

21 

Lee JS, Lee JS, Roh HL, Kim CH, Jung JS and Suh KT: Alterations in the differentiation ability of mesenchymal stem cells in patients with nontraumatic osteonecrosis of the femoral head: Comparative analysis according to the risk factor. J Orthop Res. 24:604–609. 2006. View Article : Google Scholar : PubMed/NCBI

22 

Houdek MT, Wyles CC, Packard BD, Terzic A, Behfar A and Sierra RJ: Decreased osteogenic activity of mesenchymal stem cells in patients with corticosteroid-induced osteonecrosis of the femoral head. J Arthroplasty. 31:893–898. 2016. View Article : Google Scholar

23 

Wang BL, Sun W, Shi ZC, Lou JN, Zhang NF, Shi SH, Guo WS, Cheng LM, Ye LY, Zhang WJ and Li ZR: Decreased proliferation of mesenchymal stem cells in corticosteroid-induced osteonecrosis of femoral head. Orthopedics. 31:4442008.

24 

Zhou DA, Zheng HX, Wang CW, Shi D and Li JJ: Influence of glucocorticoids on the osteogenic differentiation of rat bone marrow-derived mesenchymal stem cells. BMC Musculoskeletal Disord. 15:2392014. View Article : Google Scholar

25 

Zhang W, Yang N and Shi XM: Regulation of mesenchymal stem cell osteogenic differentiation by glucocorticoid-induced leucine zipper (GILZ). J Biol Chem. 283:4723–4729. 2008. View Article : Google Scholar

26 

Waterhouse E: Intravenous valproate for pediatric status epilepticus. Epilepsy Curr. 3:208–209. 2003. View Article : Google Scholar

27 

Göttlicher M, Minucci S, Zhu P, Krämer OH, Schimpf A, Giavara S, Sleeman JP, Lo Coco F, Nervi C, Pelicci PG and Heinzel T: Valproic acid defines a novel class of HDAC inhibitors inducing differentiation of transformed cells. EMBO J. 20:6969–6978. 2001. View Article : Google Scholar : PubMed/NCBI

28 

Cho HH, Park HT, Kim YJ, Bae YC, Suh KT and Jung JS: Induction of osteogenic differentiation of human mesenchymal stem cells by histone deacetylase inhibitors. J Cell Biochem. 96:533–542. 2005. View Article : Google Scholar : PubMed/NCBI

29 

de Ruijter AJ, van Gennip AH, Caron HN, Kemp S and van Kuilenburg AB: Histone deacetylases (HDACs): Characterization of the classical HDAC family. Biochem J. 370:737–749. 2003. View Article : Google Scholar

30 

Delcuve GP, Rastegar M and Davie JR: Epigenetic control. J Cell Physiol. 219:243–250. 2009. View Article : Google Scholar : PubMed/NCBI

31 

Zheng QF, Wang HM, Wang ZF, Liu JY, Zhang Q, Zhang L, Lu YH, You H and Jin GH: Reprogramming of histone methylation controls the differentiation of monocytes into macrophages. FEBS J. 284:1309–1323. 2017. View Article : Google Scholar : PubMed/NCBI

32 

Qin H, Zhao A, Zhang C and Fu X: Epigenetic control of reprogramming and transdifferentiation by histone modifications. Stem Cell Rev. 12:708–720. 2016. View Article : Google Scholar : PubMed/NCBI

33 

Sepulveda H, Aguilar R, Prieto CP, Bustos F, Aedo S, Lattus J, van Zundert B, Palma V and Montecino M: Epigenetic signatures at the RUNX2-P1 and Sp7 gene promoters control osteogenic lineage commitment of umbilical Cord-derived mesenchymal stem cells. J Cell Physiol. 232:2519–2527. 2017. View Article : Google Scholar

34 

Shahbazian MD and Grunstein M: Functions of site-specific histone acetylation and deacetylation. Annu Rev Biochem. 76:75–100. 2007. View Article : Google Scholar : PubMed/NCBI

35 

Hatakeyama Y, Hatakeyama J, Takahashi A, Oka K, Tsuruga E, Inai T and Sawa Y: The effect of valproic Acid on mesenchymal pluripotent cell proliferation and differentiation in extracellular matrices. Drug Target Insights. 5:1–9. 2011. View Article : Google Scholar : PubMed/NCBI

36 

Paino F, La Noce M, Tirino V, Naddeo P, Desiderio V, Pirozzi G, De Rosa A, Laino L, Altucci L and Papaccio G: Histone deacetylase inhibition with valproic acid downregulates osteocalcin gene expression in human dental pulp stem cells and osteoblasts: Evidence for HDAC2 involvement. Stem Cells. 32:279–289. 2014. View Article : Google Scholar :

37 

Xu Y, Hammerick KE, James AW, Carre AL, Leucht P, Giaccia AJ and Longaker MT: Inhibition of histone deacetylase activity in reduced oxygen environment enhances the osteogenesis of mouse adipose-derived stromal cells. Tissue Eng Part A. 15:3697–3707. 2009. View Article : Google Scholar : PubMed/NCBI

38 

Lee S, Park JR, Seo MS, Roh KH, Park SB, Hwang JW, Sun B, Seo K, Lee YS, Kang SK, et al: Histone deacetylase inhibitors decrease proliferation potential and multilineage differentiation capability of human mesenchymal stem cells. Cell Prolif. 42:711–720. 2009. View Article : Google Scholar : PubMed/NCBI

39 

Rashid MM, Akiba Y, Akiba N, Kaku M and Uoshima K: Effect of valproic acid on bone healing in rat. IADR/AADR/CADR General Session and Exhibition. 2013.

40 

Grose AW, Gardner MJ, Sussmann PS, Helfet DL and Lorich DG: The surgical anatomy of the blood supply to the femoral head: Description of the anastomosis between the medial femoral circumflex and inferior gluteal arteries at the hip. J Bone Joint Surg Br. 90:1298–1303. 2008. View Article : Google Scholar : PubMed/NCBI

41 

Zlotorowicz M and Czubak J: Vascular anatomy and blood supply to the femoral head. Osteonecrosis. 19–25. 2014.

42 

Li GY, Feng Y, Cheng TS, Yin JM and Zhang CQ: Edaravone, a novel free radical scavenger, prevents steroid-induced osteonecrosis in rabbits. Rheumatology. 52:438–447. 2013. View Article : Google Scholar

43 

Chen CH and Wang GJ: Alendronate in the prevention of collapse of the femoral head in nontraumatic osteonecrosis. Osteonecrosis. 7:265–271. 2014.

44 

Nishida K, Yamamoto T, Motomura G, Jingushi S and Iwamoto Y: Pitavastatin may reduce risk of steroid-induced osteonecrosis in rabbits: A preliminary histological study. Clin Orthop Relat Res. 466:1054–1058. 2008. View Article : Google Scholar : PubMed/NCBI

45 

Zhou D, Qi C, Chen YX, Zhu YJ, Sun TW, Chen F and Zhang CQ: Comparative study of porous hydroxyapatite/chitosan and whitlockite/chitosan scaffolds for bone regeneration in calvarial defects. Int J Nanomedicine. 12:2673–2687. 2017. View Article : Google Scholar : PubMed/NCBI

46 

Luo S, Yang Y, Chen J, Zhong Z, Huang H, Zhang J and Cui L: Tanshinol stimulates bone formation and attenuates dexamethasone-induced inhibition of osteogenesis in larval zebrafish. J Orthopaedic Translation. 4:35–45. 2015. View Article : Google Scholar

47 

Fujita T, Fukuyama R, Enomoto H and Komori T: Dexamethasone inhibits insulin-induced chondrogenesis of ATDC5 cells by preventing PI3K-Akt signaling and DNA binding of Runx2. J Cell Biochem. 93:374–83. 2004. View Article : Google Scholar : PubMed/NCBI

48 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2−ΔΔCT method. Methods. 25:402–408. 2001. View Article : Google Scholar

49 

Zhang YL, Yin JH, Ding H, Zhang W, Zhang CQ and Gao YS: Vitamin K2 prevents glucocorticoid-induced osteonecrosis of the femoral head in rats. Int J Biol Sci. 12:347–358. 2016. View Article : Google Scholar :

50 

Guo SC, Tao SC, Yin WJ, Qi X, Sheng JG and Zhang CQ: Exosomes from human synovial-derived mesenchymal stem cells prevent glucocorticoid-induced osteonecrosis of the femoral head in the rat. Int J Biol Sci. 12:1262–1272. 2016. View Article : Google Scholar : PubMed/NCBI

51 

Siéssere S, Semprini M, Lopes RA, Sala MA and Mattos MC: Morphological and morphometric alterations induced by valproic acid on rat fetuses' Meckel's cartilage, lingual musculature, and submandibular gland. Int J Morphol. 22:133–137. 2004. View Article : Google Scholar

52 

Welbat JU, Chaisawang P, Chaijaroonkhanarak W, Prachaney P, Pannangrong W, Sripanidkulchai B and Wigmore P: Kaempferia parviflora extract ameliorates the cognitive impairments and the reduction in cell proliferation induced by valproic acid treatment in rats. Ann Anat. 206:7–13. 2016. View Article : Google Scholar : PubMed/NCBI

53 

Cho HH, Park HT, Kim YJ, Bae YC, Suh KT and Jung JS: Induction of osteogenic differentiation of human mesenchymal stem cells by histone deacetylase inhibitors. J Cell Biochem. 96:533–542. 2005. View Article : Google Scholar : PubMed/NCBI

54 

Jeon MJ, Kim JA, Kwon SH, Kim SW, Park KS, Park SW, Kim SY and Shin CS: Activation of peroxisome proliferator-activated receptor-gamma inhibits the Runx2-mediated transcription of osteocalcin in osteoblasts. J Biol Chem. 278:23270–23277. 2003. View Article : Google Scholar : PubMed/NCBI

55 

Morimoto E, Li M, Khalid AB, Krum SA, Chimge NO and Frenkel B: Glucocorticoids Hijack Runx2 to stimulate Wif1 for suppression of osteoblast growth and differentiation. J Cell Physiol. 232:145–153. 2017. View Article : Google Scholar :

56 

Shi XM, Chutkan N, Hamrick MW and Isales CM: Mechanism of glucocorticoid-induced osteoporosis: An update. Intech. 2012. View Article : Google Scholar

57 

Marquez-Curtis LA, Qiu Y, Xu A and Janowska-Wieczorek A: Migration, proliferation, and differentiation of cord blood mesenchymal stromal cells treated with histone deacetylase inhibitor valproic acid. Stem Cells Int. 2014:6104952014. View Article : Google Scholar : PubMed/NCBI

58 

Tomé M, López-Romero P, Albo C, Sepúlveda JC, Fernández-Gutiérrez B, Dopazo A, Bernad A and González MA: miR-335 orchestrates cell proliferation, migration and differentiation in human mesenchymal stem cells. Cell Death Differ. 18:985–995. 2011. View Article : Google Scholar :

59 

Ciciarello M, Zini R, Rossi L, Salvestrini V, Ferrari D, Manfredini R and Lemoli RM: Extracellular purines promote the differentiation of human bone marrow-derived mesenchymal stem cells to the osteogenic and adipogenic lineages. Stem Cells Dev. 22:1097–1111. 2013. View Article : Google Scholar :

60 

Ding H, Wang T, Xu D, Cha B, Liu J and Li Y: Dexamethasone-induced apoptosis of osteocytic and osteoblastic cells is mediated by TAK1 activation. Biochem Biophys Res Commun. 460:157–163. 2015. View Article : Google Scholar : PubMed/NCBI

61 

Kim SM, Kim YG, Park JW, Lee JM and Suh JY: The effects of dexamethasone on the apoptosis and osteogenic differentiation of human periodontal ligament cells. J Periodontal Implant Sci. 43:168–176. 2013. View Article : Google Scholar : PubMed/NCBI

62 

Pang J, Huang H, Zhang Q, et al: The effect of dexamethasone on the proliferation and apoptosis of human bone marrow mesenchymal stem cells in vitro. Modern Med J China. 2008.

63 

Gao B, Huang Q, Jie Q, Zhang HY, Wang L, Guo YS, Sun Z, Wei BY, Han YH, Liu J, et al: Ginsenoside-Rb2 inhibits dexamethasone-induced apoptosis through promotion of GPR120 induction in bone marrow-derived mesenchymal stem cells. Stem Cells Dev. 24:781–790. 2015. View Article : Google Scholar

64 

Komori T: Regulation of osteoblast differentiation by Runx2. Adv Exp Med Biol. 658:43–49. 2010. View Article : Google Scholar

65 

Rimando MG, Wu HH, Liu YA, Lee CW, Kuo SW, Lo YP, Tseng KF, Liu YS and Lee OK: Glucocorticoid receptor and Histone deacetylase 6 mediate the differential effect of dexamethasone during osteogenesis of mesenchymal stromal cells (MSCs). Sci Rep. 6:373712016. View Article : Google Scholar : PubMed/NCBI

66 

Pujols L, Mullol J, Pérez M, Roca-Ferrer J, Juan M, Xaubet A, Cidlowski JA and Picado C: Expression of the human glucocorticoid receptor alpha and beta isoforms in human respiratory epithelial cells and their regulation by dexamethasone. Am J Respir Cell Mol Biol. 24:49–57. 2001. View Article : Google Scholar : PubMed/NCBI

67 

Li LB, Leung DY, Martin RJ and Goleva E: Inhibition of histone deacetylase 2 expression by elevated glucocorticoid receptor beta in steroid-resistant asthma. Am J Respir Crit Care Med. 182:877–883. 2010. View Article : Google Scholar : PubMed/NCBI

68 

Adcock IM: Glucocorticoid-regulated transcription factors. Pulm Pharmacol Ther. 14:211–219. 2001. View Article : Google Scholar : PubMed/NCBI

69 

Zhang YY, Li X, Qian SW, Guo L, Huang HY, He Q, Liu Y, Ma CG and Tang QQ: Down-regulation of type I Runx2 mediated by dexamethasone is required for 3T3-L1 adipogenesis. Mol Endocrinol. 26:798–808. 2012. View Article : Google Scholar : PubMed/NCBI

70 

Adcock IM, Cosio B, Tsaprouni L, Barnes PJ and Ito K: Redox regulation of histone deacetylases and glucocorticoid-mediated inhibition of the inflammatory response. Antioxid Redox Signal. 7:144–152. 2005. View Article : Google Scholar : PubMed/NCBI

71 

Ito K, Yamamura S, Essilfie-Quaye S, Cosio B, Ito M, Barnes PJ and Adcock IM: Histone deacetylase 2-mediated deacetylation of the glucocorticoid receptor enables NF-kappaB suppression. J Exp Med. 203:7–13. 2006. View Article : Google Scholar

72 

Okazaki S, Nagoya S, Matsumoto H, Mizuo K, Sasaki M, Watanabe S, Yamashita T and Inoue H: Development of non-traumatic osteonecrosis of the femoral head requires toll-like receptor 7 and 9 stimulations and is boosted by repression on nuclear factor kappa B in rats. Lab Invest. 95:92–99. 2015. View Article : Google Scholar

73 

Chen S, Li J, Peng H, Zhou J and Fang H: Administration of erythropoietin exerts protective effects against glucocorticoid-induced osteonecrosis of the femoral head in rats. Int J Mol Med. 33:840–848. 2014. View Article : Google Scholar : PubMed/NCBI

74 

Han N, Yan ZQ, Guo CA, Shen F, Liu J, Shi YX and Zhang ZY: Effect of rifampicin on the risk of steroid-induced osteonecrosis of the femoral head. Orthop Surg. 2:124–133. 2010. View Article : Google Scholar : PubMed/NCBI

75 

Tseng MT, Hsieh SC, Shun CT, Lee KL, Pan CL, Lin WM, Lin YH, Yu CL and Hsieh ST: Skin denervation and cutaneous vasculitis in systemic lupus erythematosus. Brain. 129:977–985. 2006. View Article : Google Scholar : PubMed/NCBI

76 

Suda S, Katsura K, Kanamaru T, Saito M and Katayama Y: Valproic acid attenuates ischemia-reperfusion injury in the rat brain through inhibition of oxidative stress and inflammation. Eur J Pharmacol. 707:26–31. 2013. View Article : Google Scholar : PubMed/NCBI

77 

Rao T, Wu F, Xing D, Peng Z, Ren D, Feng W, Chen Y, Zhao Z, Wang H, Wang J, et al: Effects of valproic Acid on axonal regeneration and recovery of motor function after peripheral nerve injury in the rat. Arch Bone Jt Surg. 2:17–24. 2014.PubMed/NCBI

78 

Zhang Z, Qin X, Tong N, Zhao X, Gong Y, Shi Y and Wu X: Valproic acid-mediated neuroprotection in retinal ischemia injury via histone deacetylase inhibition and transcriptional activation. Exp Eye Res. 94:98–108. 2012. View Article : Google Scholar

79 

Hwabejire JO, Lu J, Liu B, Li Y, Halaweish I and Alam HB: Valproic acid for the treatment of hemorrhagic shock: A dose-optimization study. J Surg Res. 186:363–370. 2014. View Article : Google Scholar

80 

Krishnamoorthy G, Karande S, Ahire N, Mathew L and Kulkarni M: Bone metabolism alteration on antiepileptic drug therapy. Indian J Pediatr. 76:377–383. 2009. View Article : Google Scholar : PubMed/NCBI

81 

Lee HS, Lee CS, Jang JS, Lee JD and Um SM: Changes of serum alkaline phosphatase and osteocalcin during fracture healing. J Korean Orthopaedic Association. 37:411–415. 2002. View Article : Google Scholar

82 

Komnenou A, Karayannopoulou M, Polizopoulou ZS, Constantinidis TC and Dessiris A: Correlation of serum alkaline phosphatase activity with the healing process of long bone fractures in dogs. Vet Clin Pathol. 34:35–38. 2005. View Article : Google Scholar : PubMed/NCBI

83 

Floerkemeier T, Hirsch S, Budde S, Radtke K, Thorey F, Windhagen H and von Lewinski G: Bone turnover markers failed to predict the occurrence of osteonecrosis of the femoral head: A preliminary study. J Clin Lab Anal. 26:55–60. 2012. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Zhou D, Chen YX, Yin JH, Tao SC, Guo SC, Wei ZY, Feng Y and Zhang CQ: Valproic acid prevents glucocorticoid‑induced osteonecrosis of the femoral head of rats. Int J Mol Med 41: 3433-3447, 2018.
APA
Zhou, D., Chen, Y., Yin, J., Tao, S., Guo, S., Wei, Z. ... Zhang, C. (2018). Valproic acid prevents glucocorticoid‑induced osteonecrosis of the femoral head of rats. International Journal of Molecular Medicine, 41, 3433-3447. https://doi.org/10.3892/ijmm.2018.3534
MLA
Zhou, D., Chen, Y., Yin, J., Tao, S., Guo, S., Wei, Z., Feng, Y., Zhang, C."Valproic acid prevents glucocorticoid‑induced osteonecrosis of the femoral head of rats". International Journal of Molecular Medicine 41.6 (2018): 3433-3447.
Chicago
Zhou, D., Chen, Y., Yin, J., Tao, S., Guo, S., Wei, Z., Feng, Y., Zhang, C."Valproic acid prevents glucocorticoid‑induced osteonecrosis of the femoral head of rats". International Journal of Molecular Medicine 41, no. 6 (2018): 3433-3447. https://doi.org/10.3892/ijmm.2018.3534
Copy and paste a formatted citation
x
Spandidos Publications style
Zhou D, Chen YX, Yin JH, Tao SC, Guo SC, Wei ZY, Feng Y and Zhang CQ: Valproic acid prevents glucocorticoid‑induced osteonecrosis of the femoral head of rats. Int J Mol Med 41: 3433-3447, 2018.
APA
Zhou, D., Chen, Y., Yin, J., Tao, S., Guo, S., Wei, Z. ... Zhang, C. (2018). Valproic acid prevents glucocorticoid‑induced osteonecrosis of the femoral head of rats. International Journal of Molecular Medicine, 41, 3433-3447. https://doi.org/10.3892/ijmm.2018.3534
MLA
Zhou, D., Chen, Y., Yin, J., Tao, S., Guo, S., Wei, Z., Feng, Y., Zhang, C."Valproic acid prevents glucocorticoid‑induced osteonecrosis of the femoral head of rats". International Journal of Molecular Medicine 41.6 (2018): 3433-3447.
Chicago
Zhou, D., Chen, Y., Yin, J., Tao, S., Guo, S., Wei, Z., Feng, Y., Zhang, C."Valproic acid prevents glucocorticoid‑induced osteonecrosis of the femoral head of rats". International Journal of Molecular Medicine 41, no. 6 (2018): 3433-3447. https://doi.org/10.3892/ijmm.2018.3534
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team