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International Journal of Molecular Medicine
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Article

CFTR ameliorates high glucose-induced oxidative stress and inflammation by mediating the NF-κB and MAPK signaling pathways in endothelial cells

  • Authors:
    • Yang Fei
    • Liqin Sun
    • Chungang Yuan
    • Min Jiang
    • Qinhua Lou
    • Yan Xu
  • View Affiliations / Copyright

    Affiliations: Department of Endocrinology, Fuyang First People's Hospital, Hangzhou, Zhejiang 311400, P.R. China
  • Pages: 3501-3508
    |
    Published online on: March 7, 2018
       https://doi.org/10.3892/ijmm.2018.3547
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Abstract

Diabetic cardiovascular diseases are characterized by progressive hyperglycemia, which results in excessive production of oxidative stress and pro-inflammatory cytokines. Cystic fibrosis (CF) is characterized by chronic inflammation due to mutations in CF transmembrane conductance regulator (CFTR). However, little information is available about the role of CFTR in hyperglycemia‑induced endothelial cell oxidative stress and inflammation. In the present study, a high glucose‑treatment was applied in human umbilical vein endothelial cells with CFTR overexpression or inhibition, and the oxidative and inflammatory characteristics were measured. It was shown that CFTR protein and mRNA expression were reduced by glucose in a concentration‑dependent manner. Overexpression of CFRT via adenoviral infection significantly inhibited the production of reactive oxygen species and inflammatory biomediators induced by high glucose. Conversely, pharmacological inhibition of CFTR led to the opposite effects. Mechanistically, nuclear factor‑κB (NF‑κB) and mitogen‑activated protein kinase (MAPK) signaling were activated following high glucose treatment, which were inhibited by CFTR overexpression and enhanced by CFTR inhibition. The pro‑inflammatory effect of CFTR inhibition was abolished by pharmacological inhibition of the NF‑κB or MAPK pathways. Moreover, inhibition of MAPK abrogated CFTR inhibition‑induced NF‑κB nuclear translocation, whereas NF‑κB inhibitor produced no effects on MAPK activation. Additionally, antioxidant treatment inhibited the high glucose‑induced decrease in CFTR expression and the increase in inflammatory responses. Collectively, these findings revealed that CFTR attenuates high glucose‑induced endothelial cell oxidative stress and inflammation through inactivation of NF‑κB and MAPK signaling, indicating that elevation of CFRT expression may be a novel strategy in preventing endothelial dysfunction in diabetes.
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Copy and paste a formatted citation
Spandidos Publications style
Fei Y, Sun L, Yuan C, Jiang M, Lou Q and Xu Y: CFTR ameliorates high glucose-induced oxidative stress and inflammation by mediating the NF-κB and MAPK signaling pathways in endothelial cells. Int J Mol Med 41: 3501-3508, 2018.
APA
Fei, Y., Sun, L., Yuan, C., Jiang, M., Lou, Q., & Xu, Y. (2018). CFTR ameliorates high glucose-induced oxidative stress and inflammation by mediating the NF-κB and MAPK signaling pathways in endothelial cells. International Journal of Molecular Medicine, 41, 3501-3508. https://doi.org/10.3892/ijmm.2018.3547
MLA
Fei, Y., Sun, L., Yuan, C., Jiang, M., Lou, Q., Xu, Y."CFTR ameliorates high glucose-induced oxidative stress and inflammation by mediating the NF-κB and MAPK signaling pathways in endothelial cells". International Journal of Molecular Medicine 41.6 (2018): 3501-3508.
Chicago
Fei, Y., Sun, L., Yuan, C., Jiang, M., Lou, Q., Xu, Y."CFTR ameliorates high glucose-induced oxidative stress and inflammation by mediating the NF-κB and MAPK signaling pathways in endothelial cells". International Journal of Molecular Medicine 41, no. 6 (2018): 3501-3508. https://doi.org/10.3892/ijmm.2018.3547
Copy and paste a formatted citation
x
Spandidos Publications style
Fei Y, Sun L, Yuan C, Jiang M, Lou Q and Xu Y: CFTR ameliorates high glucose-induced oxidative stress and inflammation by mediating the NF-κB and MAPK signaling pathways in endothelial cells. Int J Mol Med 41: 3501-3508, 2018.
APA
Fei, Y., Sun, L., Yuan, C., Jiang, M., Lou, Q., & Xu, Y. (2018). CFTR ameliorates high glucose-induced oxidative stress and inflammation by mediating the NF-κB and MAPK signaling pathways in endothelial cells. International Journal of Molecular Medicine, 41, 3501-3508. https://doi.org/10.3892/ijmm.2018.3547
MLA
Fei, Y., Sun, L., Yuan, C., Jiang, M., Lou, Q., Xu, Y."CFTR ameliorates high glucose-induced oxidative stress and inflammation by mediating the NF-κB and MAPK signaling pathways in endothelial cells". International Journal of Molecular Medicine 41.6 (2018): 3501-3508.
Chicago
Fei, Y., Sun, L., Yuan, C., Jiang, M., Lou, Q., Xu, Y."CFTR ameliorates high glucose-induced oxidative stress and inflammation by mediating the NF-κB and MAPK signaling pathways in endothelial cells". International Journal of Molecular Medicine 41, no. 6 (2018): 3501-3508. https://doi.org/10.3892/ijmm.2018.3547
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