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Article

Rhododendron album Blume extract inhibits TNF-α/IFN-γ-induced chemokine production via blockade of NF-κB and JAK/STAT activation in human epidermal keratinocytes

  • Authors:
    • Ji‑Won Park
    • Han‑Sol Lee
    • Yourim Lim
    • Jin‑Hyub Paik
    • Ok‑Kyoung Kwon
    • Jung‑Hee Kim
    • Imam Paryanto
    • Prasetyawan Yunianto
    • Sangho Choi
    • Sei‑Ryang Oh
    • Kyung‑Seop Ahn
  • View Affiliations / Copyright

    Affiliations: Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Cheongju, Chungbuk 28116, Republic of Korea, International Biological Material Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 34141, Republic of Korea, Center for Pharmaceutical and Medical Technology, Kawasan Puspiptek Serpong, LAPTIAB, Tangerang, Banten 15314, Indonesia
  • Pages: 3642-3652
    |
    Published online on: March 9, 2018
       https://doi.org/10.3892/ijmm.2018.3556
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Abstract

Rhododendron album Blume (RA) has traditionally been used as an herbal medicine and is considered to have anti‑inflammatory properties. It is a well‑known medicine for treatment of allergic or atopic diseases. In the present study, the biological effects of an RA methanol extract (RAME) on inflammation were investigated in tumor necrosis factor‑α (TNF‑α)/interferon‑γ (IFN‑γ)‑stimulated human keratinocytes. The present study aimed to investigate the potential mechanisms by which RAME inhibited TNF‑α/IFN‑γ‑induced expression of chemokines [thymus‑ and activation-regulated chemokine (TARC) and macrophage‑derived chemokine (MDC)] and cytokines [interleukin (IL)‑6 and IL‑8] through the nuclear factor‑κB (NF‑κB) pathway in human keratinocytes. The effects of RAME treatment on cell viability were investigated in TNF‑α/IFN‑γ‑stimulated HaCaT cells. The expression of TARC, MDC, IL‑6 and IL‑8 was assessed using reverse transcription‑quantitative polymerase chain reaction analysis or ELISA, and its effect on the inhibitory mitogen-activated protein kinase pathway was also studied using western blot analysis. TNF‑α/IFN‑γ induced the expression of IL‑6, IL‑8, TARC and MDC in a dose‑dependent manner through NF‑κB and Janus kinase/signal transducers and activators of transcription (JAK/STAT) activation. Notably, treatment with RAME significantly suppressed TNF-α/IFN-γ-induced expression of IL‑6, IL‑8, TARC, and MDC. In addition, RAME treatment inhibited the activation of NF‑κB and the JAK/STAT pathway in TNF‑α/IFN‑γ‑induced HaCaT cells. These results suggest that RAME decreases the production of chemokines and pro‑inflammatory cytokines by suppressing the NF‑κB and the JAK/STAT pathways. Consequently, RAME may potentially be used for treatment of atopic dermatitis.
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Copy and paste a formatted citation
Spandidos Publications style
Park JW, Lee HS, Lim Y, Paik JH, Kwon OK, Kim JH, Paryanto I, Yunianto P, Choi S, Oh SR, Oh SR, et al: Rhododendron album Blume extract inhibits TNF-α/IFN-γ-induced chemokine production via blockade of NF-κB and JAK/STAT activation in human epidermal keratinocytes. Int J Mol Med 41: 3642-3652, 2018.
APA
Park, J., Lee, H., Lim, Y., Paik, J., Kwon, O., Kim, J. ... Ahn, K. (2018). Rhododendron album Blume extract inhibits TNF-α/IFN-γ-induced chemokine production via blockade of NF-κB and JAK/STAT activation in human epidermal keratinocytes. International Journal of Molecular Medicine, 41, 3642-3652. https://doi.org/10.3892/ijmm.2018.3556
MLA
Park, J., Lee, H., Lim, Y., Paik, J., Kwon, O., Kim, J., Paryanto, I., Yunianto, P., Choi, S., Oh, S., Ahn, K."Rhododendron album Blume extract inhibits TNF-α/IFN-γ-induced chemokine production via blockade of NF-κB and JAK/STAT activation in human epidermal keratinocytes". International Journal of Molecular Medicine 41.6 (2018): 3642-3652.
Chicago
Park, J., Lee, H., Lim, Y., Paik, J., Kwon, O., Kim, J., Paryanto, I., Yunianto, P., Choi, S., Oh, S., Ahn, K."Rhododendron album Blume extract inhibits TNF-α/IFN-γ-induced chemokine production via blockade of NF-κB and JAK/STAT activation in human epidermal keratinocytes". International Journal of Molecular Medicine 41, no. 6 (2018): 3642-3652. https://doi.org/10.3892/ijmm.2018.3556
Copy and paste a formatted citation
x
Spandidos Publications style
Park JW, Lee HS, Lim Y, Paik JH, Kwon OK, Kim JH, Paryanto I, Yunianto P, Choi S, Oh SR, Oh SR, et al: Rhododendron album Blume extract inhibits TNF-α/IFN-γ-induced chemokine production via blockade of NF-κB and JAK/STAT activation in human epidermal keratinocytes. Int J Mol Med 41: 3642-3652, 2018.
APA
Park, J., Lee, H., Lim, Y., Paik, J., Kwon, O., Kim, J. ... Ahn, K. (2018). Rhododendron album Blume extract inhibits TNF-α/IFN-γ-induced chemokine production via blockade of NF-κB and JAK/STAT activation in human epidermal keratinocytes. International Journal of Molecular Medicine, 41, 3642-3652. https://doi.org/10.3892/ijmm.2018.3556
MLA
Park, J., Lee, H., Lim, Y., Paik, J., Kwon, O., Kim, J., Paryanto, I., Yunianto, P., Choi, S., Oh, S., Ahn, K."Rhododendron album Blume extract inhibits TNF-α/IFN-γ-induced chemokine production via blockade of NF-κB and JAK/STAT activation in human epidermal keratinocytes". International Journal of Molecular Medicine 41.6 (2018): 3642-3652.
Chicago
Park, J., Lee, H., Lim, Y., Paik, J., Kwon, O., Kim, J., Paryanto, I., Yunianto, P., Choi, S., Oh, S., Ahn, K."Rhododendron album Blume extract inhibits TNF-α/IFN-γ-induced chemokine production via blockade of NF-κB and JAK/STAT activation in human epidermal keratinocytes". International Journal of Molecular Medicine 41, no. 6 (2018): 3642-3652. https://doi.org/10.3892/ijmm.2018.3556
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