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International Journal of Molecular Medicine
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Print ISSN: 1107-3756 Online ISSN: 1791-244X
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June-2018 Volume 41 Issue 6

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International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

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International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

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Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

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Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

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Article

Interfering RNA with multi-targets for efficient gene suppression in HCC cells

  • Authors:
    • Tiejun Li
    • York Yuanyuan Zhu
    • Yi Ji
    • Songfeng Zhou
  • View Affiliations / Copyright

    Affiliations: Small RNA Technology and Application Institute, Nantong University, Nantong 226016, P.R. China
  • Pages: 3604-3610
    |
    Published online on: March 9, 2018
       https://doi.org/10.3892/ijmm.2018.3557
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Abstract

RNA interference (RNAi) technology has been widely used in therapeutics development, especially multiple targeted RNAi strategy, which is a better method for multiple gene suppression. In the study, interfering RNAs (iRNAs) were designed for carrying two or three different siRNA sequences in different secondary structure formats (loop or cloverleaf). By using these types of iRNAs, co-inhibition of survivin and B-cell lymphoma-2 (Bcl-2) was investigated in hepatocellular carcinoma (HCC) cells, and we obtained promising gene silencing effects without showing undesirable interferon response. Furthermore, suppression effects on proliferation, invasion, and induced apoptosis in HCC cells were validated. The results suggest that long iRNAs with secondary structure may be a preferred strategy for multigenic disease therapy, especially for cancer and viral gene therapy and their iRNA drug development.
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Copy and paste a formatted citation
Spandidos Publications style
Li T, Zhu YY, Ji Y and Zhou S: Interfering RNA with multi-targets for efficient gene suppression in HCC cells. Int J Mol Med 41: 3604-3610, 2018.
APA
Li, T., Zhu, Y.Y., Ji, Y., & Zhou, S. (2018). Interfering RNA with multi-targets for efficient gene suppression in HCC cells. International Journal of Molecular Medicine, 41, 3604-3610. https://doi.org/10.3892/ijmm.2018.3557
MLA
Li, T., Zhu, Y. Y., Ji, Y., Zhou, S."Interfering RNA with multi-targets for efficient gene suppression in HCC cells". International Journal of Molecular Medicine 41.6 (2018): 3604-3610.
Chicago
Li, T., Zhu, Y. Y., Ji, Y., Zhou, S."Interfering RNA with multi-targets for efficient gene suppression in HCC cells". International Journal of Molecular Medicine 41, no. 6 (2018): 3604-3610. https://doi.org/10.3892/ijmm.2018.3557
Copy and paste a formatted citation
x
Spandidos Publications style
Li T, Zhu YY, Ji Y and Zhou S: Interfering RNA with multi-targets for efficient gene suppression in HCC cells. Int J Mol Med 41: 3604-3610, 2018.
APA
Li, T., Zhu, Y.Y., Ji, Y., & Zhou, S. (2018). Interfering RNA with multi-targets for efficient gene suppression in HCC cells. International Journal of Molecular Medicine, 41, 3604-3610. https://doi.org/10.3892/ijmm.2018.3557
MLA
Li, T., Zhu, Y. Y., Ji, Y., Zhou, S."Interfering RNA with multi-targets for efficient gene suppression in HCC cells". International Journal of Molecular Medicine 41.6 (2018): 3604-3610.
Chicago
Li, T., Zhu, Y. Y., Ji, Y., Zhou, S."Interfering RNA with multi-targets for efficient gene suppression in HCC cells". International Journal of Molecular Medicine 41, no. 6 (2018): 3604-3610. https://doi.org/10.3892/ijmm.2018.3557
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