Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
International Journal of Molecular Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1107-3756 Online ISSN: 1791-244X
Journal Cover
June-2018 Volume 41 Issue 6

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
June-2018 Volume 41 Issue 6

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Islet-1 promotes the proliferation and invasion, and inhibits the apoptosis of A375 human melanoma cells

  • Authors:
    • Xiaoling Zhu
    • Yuzhen Li
    • Qinggang Meng
  • View Affiliations / Copyright

    Affiliations: Department of Dermatology, The First Hospital of Harbin, Harbin, Heilongjiang 150010, P.R. China, Department of Dermatology, The Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150081, P.R. China, Department of Orthopaedic Surgery, The First Hospital of Harbin, Harbin, Heilongjiang 150010, P.R. China
  • Pages: 3680-3690
    |
    Published online on: March 14, 2018
       https://doi.org/10.3892/ijmm.2018.3569
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

The aim of this study was to examine the effects of the insulin gene enhancer-binding protein, islet-1 (ISL1), on the proliferation, invasion and apoptosis of the human melanoma cell line, A375. An ISL1 overexpression lentiviral vector was constructed and transfected into the A375 cells. The proliferation of the A375 cells transfected with the ISL1 vector (termed A375/ISL1 cells) was examined by MTT assay, flow cytometry and TUNEL assay, and cell invasion was examined by Transwell assay. The expression levels of matrix metalloproteinase (MMP)-2 and MMP-9 were measured by qPCR and western blot analysis; the expression levels of Akt and p-Akt were measured in the cells treated with vascular endothelial growth factor (VEGF) and the PI3K/Akt inhibitor, LY294002, by western blot analysis. The optical density value of the A375/ISL1 cells was increased after 12 h of culture (P<0.001), as shown by MTT assay. The ratio of apoptotic A375/ISL1 cells was significantly decreased (P<0.001), as shown by flow cytometry and TUNEL assay. In addition, the average penetration rate of the A375/ISL1 cells significantly increased (P<0.001), as shown by Transwell assay. The expression levels of MMP-2 and MMP-9 were significantly increased in the A375/ISL1 cells, as shown by qPCR and western blot analysis (P<0.001). Moreover, treatment of the A375/ISL1 cells with VEGF for 48 h increased the expression of Akt and p-Akt compared with the control cells transfected with A375/green fluorescent protein (GFP) (P<0.05; P<0.001, respectively). In addition, in the A375/ISL1 cells treated with the LY294002 inhibitor for 24 and 48 h, the level of Akt was also found to increase compared to the control A375/GFP cells (P<0.05). On the whole, the findings of this study indicate that the overexpression of ISL1 promotes the proliferation and invasion, and inhibits the apoptosis of A375 melanoma cells. ISL1 thus plays an important role in A375 cell survival, and these effects are possibly mediate via the PI3K/Akt signaling pathway.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

Figure 7

Figure 8

Figure 9

Figure 10

Figure 11

View References

1 

Misu M, Ouji Y, Kawai N, Nishimura F, Nakamura-Uchiyama F and Yoshikawa M: Effects of Wnt-10b on proliferation and differentiation of murine melanoma cells. Biochem Biophys Res Commun. 463:618–623. 2015. View Article : Google Scholar : PubMed/NCBI

2 

Raghunath A, Sambarey A, Sharma N, Mahadevan U and Chandra N: A molecular systems approach to modelling human skin pigmentation: identifying underlying pathways and critical components. BMC Res Notes. 8:1702015. View Article : Google Scholar : PubMed/NCBI

3 

Siegel R, Ma J, Zou Z and Jemal A: Cancer statistics, 2014. CA Cancer J Clin. 64:9–29. 2014. View Article : Google Scholar : PubMed/NCBI

4 

Karlsson O, Thor S, Norberg T, Ohlsson H and Edlund T: Insulin gene enhancer binding protein Isl-1 is a member of a novel class of proteins containing both a homeo- and a Cys-His domain. Nature. 344:879–882. 1990. View Article : Google Scholar : PubMed/NCBI

5 

Nasif S, de Souza FS, González LE, Yamashita M, Orquera DP, Low MJ and Rubinstein M: Islet 1 specifies the identity of hypothalamic melanocortin neurons and is critical for normal food intake and adiposity in adulthood. Proc Natl Acad Sci USA. 112:E1861–E1870. 2015. View Article : Google Scholar : PubMed/NCBI

6 

Cai CL, Liang X, Shi Y, Chu PH, Pfaff SL, Chen J and Evans S: Isl1 identifies a cardiac progenitor population that proliferates prior to differentiation and contributes a majority of cells to the heart. Dev Cell. 5:877–889. 2003. View Article : Google Scholar : PubMed/NCBI

7 

Huber GF: Modern management of Merkel cell carcinoma. Curr Opin Otolaryngol Head Neck Surg. 22:109–115. 2014. View Article : Google Scholar : PubMed/NCBI

8 

Agaimy A, Erlenbach-Wünsch K, Konukiewitz B, Schmitt AM, Rieker RJ, Vieth M, Kiesewetter F, Hartmann A, Zamboni G, Perren A, et al: ISL1 expression is not restricted to pancreatic well-differentiated neuroendocrine neoplasms, but is also commonly found in well and poorly differentiated neuroendocrine neoplasms of extrapancreatic origin. Mod Pathol. 26:995–1003. 2013. View Article : Google Scholar : PubMed/NCBI

9 

Su X, Wang P, Wang X, Cao B, Li L and Liu Q: Apoptosis of U937 cells induced by hematoporphyrin monomethyl ether-mediated sonodynamic action. Cancer Biother Radiopharm. 28:207–217. 2013. View Article : Google Scholar : PubMed/NCBI

10 

Hao Q, Li W, Zhang C, Qin X, Xue X, Li M, Shu Z, Xu T, Xu Y, Wang W, et al: TNFα induced FOXP3-NFκB interaction dampens the tumor suppressor role of FOXP3 in gastric cancer cells. Biochem Biophys Res Commun. 430:436–441. 2013. View Article : Google Scholar

11 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar

12 

Piérard-Franchimont C, Hermanns-Lê T, Delvenne P and Piérard GE: Dormancy of growth-stunted malignant melanoma: sustainable and smoldering patterns. Oncol Rev. 8:2522014. View Article : Google Scholar

13 

Aris M and Barrio MM: Combining immunotherapy with oncogene-targeted therapy: a new road for melanoma treatment. Front Immunol. 6:462015. View Article : Google Scholar : PubMed/NCBI

14 

Graham RP, Shrestha B, Caron BL, Smyrk TC, Grogg KL, Lloyd RV and Zhang L: Islet-1 is a sensitive but not entirely specific marker for pancreatic neuroendocrine neoplasms and their metastases. Am J Surg Pathol. 37:399–405. 2013. View Article : Google Scholar : PubMed/NCBI

15 

Bu L, Jiang X, Martin-Puig S, Caron L, Zhu S, Shao Y, Roberts DJ, Huang PL, Domian IJ and Chien KR: Human ISL1 heart progenitors generate diverse multipotent cardiovascular cell lineages. Nature. 460:113–117. 2009. View Article : Google Scholar : PubMed/NCBI

16 

Shi Y, Zhao S, Li J and Mao B: Islet-1 is required for ventral neuron survival in Xenopus. Biochem Biophys Res Commun. 388:506–510. 2009. View Article : Google Scholar : PubMed/NCBI

17 

Guo T, Wang W, Zhang H, Liu Y, Chen P, Ma K and Zhou C: ISL1 promotes pancreatic islet cell proliferation. PLoS One. 6:e223872011. View Article : Google Scholar : PubMed/NCBI

18 

Ediger BN, Du A, Liu J, Hunter CS, Walp ER, Schug J, Kaestner KH, Stein R, Stoffers DA and May CL: Islet-1 is essential for pancreatic β-cell function. Diabetes. 63:4206–4217. 2014. View Article : Google Scholar : PubMed/NCBI

19 

Weinberger F, Mehrkens D, Starbatty J, Nicol P and Eschenhagen T: Assessment of DNA synthesis in Islet-1+ cells in the adult murine heart. Biochem Biophys Res Commun. 456:294–297. 2015. View Article : Google Scholar

20 

Kilk K, Magzoub M, Pooga M, Eriksson LE, Langel U and Gräslund A: Cellular internalization of a cargo complex with a novel peptide derived from the third helix of the islet-1 homeodomain. Comparison with the penetratin peptide. Bioconjug Chem. 12:911–916. 2001. View Article : Google Scholar : PubMed/NCBI

21 

Koo J, Mertens RB, Mirocha JM, Wang HL and Dhall D: Value of Islet 1 and PAX8 in identifying metastatic neuroendocrine tumors of pancreatic origin. Mod Pathol. 25:893–901. 2012. View Article : Google Scholar : PubMed/NCBI

22 

Ferrara N, Carver-Moore K, Chen H, Dowd M, Lu L, O'Shea KS, Powell-Braxton L, Hillan KJ and Moore MW: Heterozygous embryonic lethality induced by targeted inactivation of the VEGF gene. Nature. 380:439–442. 1996. View Article : Google Scholar : PubMed/NCBI

23 

Barzelay A, Ben-Shoshan J, Entin-Meer M, Maysel-Auslender S, Afek A, Barshack I, Keren G and George J: A potential role for islet-1 in post-natal angiogenesis and vasculogenesis. Thromb Haemost. 103:188–197. 2010. View Article : Google Scholar : PubMed/NCBI

24 

Linning KD, Tai MH, Madhukar BV, Chang CC, Reed DN Jr, Ferber S, Trosko JE and Olson LK: Redox-mediated enrichment of self-renewing adult human pancreatic cells that possess endocrine differentiation potential. Pancreas. 29:e64–e76. 2004. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Zhu X, Li Y and Meng Q: Islet-1 promotes the proliferation and invasion, and inhibits the apoptosis of A375 human melanoma cells. Int J Mol Med 41: 3680-3690, 2018.
APA
Zhu, X., Li, Y., & Meng, Q. (2018). Islet-1 promotes the proliferation and invasion, and inhibits the apoptosis of A375 human melanoma cells. International Journal of Molecular Medicine, 41, 3680-3690. https://doi.org/10.3892/ijmm.2018.3569
MLA
Zhu, X., Li, Y., Meng, Q."Islet-1 promotes the proliferation and invasion, and inhibits the apoptosis of A375 human melanoma cells". International Journal of Molecular Medicine 41.6 (2018): 3680-3690.
Chicago
Zhu, X., Li, Y., Meng, Q."Islet-1 promotes the proliferation and invasion, and inhibits the apoptosis of A375 human melanoma cells". International Journal of Molecular Medicine 41, no. 6 (2018): 3680-3690. https://doi.org/10.3892/ijmm.2018.3569
Copy and paste a formatted citation
x
Spandidos Publications style
Zhu X, Li Y and Meng Q: Islet-1 promotes the proliferation and invasion, and inhibits the apoptosis of A375 human melanoma cells. Int J Mol Med 41: 3680-3690, 2018.
APA
Zhu, X., Li, Y., & Meng, Q. (2018). Islet-1 promotes the proliferation and invasion, and inhibits the apoptosis of A375 human melanoma cells. International Journal of Molecular Medicine, 41, 3680-3690. https://doi.org/10.3892/ijmm.2018.3569
MLA
Zhu, X., Li, Y., Meng, Q."Islet-1 promotes the proliferation and invasion, and inhibits the apoptosis of A375 human melanoma cells". International Journal of Molecular Medicine 41.6 (2018): 3680-3690.
Chicago
Zhu, X., Li, Y., Meng, Q."Islet-1 promotes the proliferation and invasion, and inhibits the apoptosis of A375 human melanoma cells". International Journal of Molecular Medicine 41, no. 6 (2018): 3680-3690. https://doi.org/10.3892/ijmm.2018.3569
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team