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International Journal of Molecular Medicine
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Article Open Access

miR-19a/b and MeCP2 repress reciprocally to regulate multidrug resistance in gastric cancer cells

  • Authors:
    • Fei Zhu
    • Qiong Wu
    • Zhen Ni
    • Chao Lei
    • Ting Li
    • Yongquan Shi
  • View Affiliations / Copyright

    Affiliations: State Key Laboratory of Cancer Biology and Institute of Digestive Diseases, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi 710032, P.R. China
    Copyright: © Zhu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 228-236
    |
    Published online on: March 22, 2018
       https://doi.org/10.3892/ijmm.2018.3581
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Abstract

Despite the improvement in gastric cancer (GC) treatment, multidrug resistance (MDR) is still a significant reason for chemotherapy failure. Our previous studies have demonstrated that miR-19a/b upregulation directly promoted MDR in GC cells. However, the exact regulation and the potential molecule mechanisms have not been fully clarified. In this study, we found that miR-19a/b was directly involved in 5-aza-2'-deoxycytidine (5-Aza-dC) induced MDR of GC cells. Mechanically, demethylation of miR-19a/b repressed methyl CpG binding protein 2 (MeCP2) expression via direct binding at the 3'-untranslated regions, which then alleviated the inhibitory effects of MeCP2 on miR-19a/b expression. Thus, the mutual regulatory network sustains preservation of the expression levels of miR-19a/b. We further demonstrated that miR-19a/b expression was inversely correlated to MeCP2 expression in GC tissues. These data showed an intimate interplay among miR-19a/b methylation, MeCP2 activity, and MDR, revealing a potential therapeutic target for GC.
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1 

Allemani C, Weir HK, Carreira H, Harewood R, Spika D, Wang XS, Bannon F, Ahn JV, Johnson CJ, Bonaventure A, et al CONCORD Working Group: Global surveillance of cancer survival 1995–2009: Analysis of individual data for 25,676,887 patients from 279 population-based registries in 67 countries (CONCORD-2). Lancet. 385:977–1010. 2015. View Article : Google Scholar

2 

Wu Q, Yang Z, Nie Y, Shi Y and Fan D: Multi-drug resistance in cancer chemotherapeutics: Mechanisms and lab approaches. Cancer Lett. 347:159–166. 2014. View Article : Google Scholar : PubMed/NCBI

3 

Hashimoto Y, Akiyama Y and Yuasa Y: Multiple-to-multiple relationships between microRNAs and target genes in gastric cancer. PLoS One. 8:e625892013. View Article : Google Scholar : PubMed/NCBI

4 

Guo X, Xia J and Yan J: Promoter methylated microRNAs: Potential therapeutic targets in gastric cancer. Mol Med Rep. 11:759–765. 2015. View Article : Google Scholar

5 

Olive V, Bennett MJ, Walker JC, Ma C, Jiang I, Cordon-Cardo C, Li QJ, Lowe SW, Hannon GJ and He L: miR-19 is a key oncogenic component of mir-17–92. Genes Dev. 23:2839–2849. 2009. View Article : Google Scholar : PubMed/NCBI

6 

Olive V, Jiang I and He L: mir-17–92, a cluster of miRNAs in the midst of the cancer network. Int J Biochem Cell Biol. 42:1348–1354. 2010. View Article : Google Scholar : PubMed/NCBI

7 

Woods K, Thomson JM and Hammond SM: Direct regulation of an oncogenic micro-RNA cluster by E2F transcription factors. J Biol Chem. 282:2130–2134. 2007. View Article : Google Scholar

8 

Tili E, Michaille JJ, Liu CG, Alder H, Taccioli C, Volinia S, Calin GA and Croce CM: GAM/ZFp/ZNF512B is central to a gene sensor circuitry involving cell-cycle regulators, TGF{beta} effectors, Drosha and microRNAs with opposite oncogenic potentials. Nucleic Acids Res. 38:7673–7688. 2010. View Article : Google Scholar : PubMed/NCBI

9 

Dews M, Homayouni A, Yu D, Murphy D, Sevignani C, Wentzel E, Furth EE, Lee WM, Enders GH, Mendell JT, et al: Augmentation of tumor angiogenesis by a Myc-activated microRNA cluster. Nat Genet. 38:1060–1065. 2006. View Article : Google Scholar : PubMed/NCBI

10 

Dakhlallah D, Batte K, Wang Y, Cantemir-Stone CZ, Yan P, Nuovo G, Mikhail A, Hitchcock CL, Wright VP, Nana-Sinkam SP, et al: Epigenetic regulation of miR-17~92 contributes to the pathogenesis of pulmonary fibrosis. Am J Respir Crit Care Med. 187:397–405. 2013. View Article : Google Scholar : PubMed/NCBI

11 

Wu Q, Yang Z, Xia L, Nie Y, Wu K, Shi Y and Fan D: Methylation of miR-129-5p CpG island modulates multi-drug resistance in gastric cancer by targeting ABC transporters. Oncotarget. 5:11552–11563. 2014. View Article : Google Scholar : PubMed/NCBI

12 

Wang F, Li T, Zhang B, Li H, Wu Q, Yang L, Nie Y, Wu K, Shi Y and Fan D: MicroRNA-19a/b regulates multidrug resistance in human gastric cancer cells by targeting PTEN. Biochem Biophys Res Commun. 434:688–694. 2013. View Article : Google Scholar : PubMed/NCBI

13 

Guo J, Miao Y, Xiao B, Huan R, Jiang Z, Meng D and Wang Y: Differential expression of microRNA species in human gastric cancer versus non-tumorous tissues. J Gastroenterol Hepatol. 24:652–657. 2009. View Article : Google Scholar : PubMed/NCBI

14 

Wu Q, Yang Z, Wang F, Hu S, Yang L, Shi Y and Fan D: MiR-19b/20a/92a regulates the self-renewal and proliferation of gastric cancer stem cells. J Cell Sci. 126:4220–4229. 2013. View Article : Google Scholar : PubMed/NCBI

15 

Wu Q, Yang Z, An Y, Hu H, Yin J, Zhang P, Nie Y, Wu K, Shi Y and Fan D: MiR-19a/b modulate the metastasis of gastric cancer cells by targeting the tumour suppressor MXD1. Cell Death Dis. 5:e11442014. View Article : Google Scholar : PubMed/NCBI

16 

Wang X, Gao H, Ren L, Gu J and Zhang Y and Zhang Y: Demethylation of the miR-146a promoter by 5-Aza-2′-deoxycytidine correlates with delayed progression of castration-resistant prostate cancer. BMC Cancer. 14:3082014. View Article : Google Scholar

17 

Liu J, Zhang X, Liu A, Zhang D, Su Y, Liu Y, You D, Yuan L, Kong X, Wang X, et al: Altered methylation of glucosylceramide synthase promoter regulates its expression and associates with acquired multidrug resistance in invasive ductal breast cancer. Oncotarget. 7:36755–36766. 2016.PubMed/NCBI

18 

Chen KG and Sikic BI: Molecular pathways: Regulation and therapeutic implications of multidrug resistance. Clin Cancer Res. 18:1863–1869. 2012. View Article : Google Scholar : PubMed/NCBI

19 

Fang JY, Lu R, Mikovits JA, Cheng ZH, Zhu HY and Chen YX: Regulation of hMSH2 and hMLH1 expression in the human colon cancer cell line SW1116 by DNA methyltransferase 1. Cancer Lett. 233:124–130. 2006. View Article : Google Scholar : PubMed/NCBI

20 

Liu MX, Siu MK, Liu SS, Yam JW, Ngan HY and Chan DW: Epigenetic silencing of microRNA-199b-5p is associated with acquired chemoresistance via activation of JAG1-Notch1 signaling in ovarian cancer. Oncotarget. 5:944–958. 2014. View Article : Google Scholar : PubMed/NCBI

21 

Neupane M, Clark AP, Landini S, Birkbak NJ, Eklund AC, Lim E, Culhane AC, Barry WT, Schumacher SE, Beroukhim R, et al: MECP2 is a frequently amplified oncogene with a novel epigenetic mechanism that mimics the role of activated RAS in malignancy. Cancer Discov. 6:45–58. 2016. View Article : Google Scholar :

22 

Du Q, Luu PL, Stirzaker C and Clark SJ: Methyl-CpG-binding domain proteins: Readers of the epigenome. Epigenomics. 7:1051–1073. 2015. View Article : Google Scholar : PubMed/NCBI

23 

Sansom OJ, Maddison K and Clarke AR: Mechanisms of disease: Methyl-binding domain proteins as potential therapeutic targets in cancer. Nat Clin Pract Oncol. 4:305–315. 2007. View Article : Google Scholar : PubMed/NCBI

24 

El-Osta A, Kantharidis P, Zalcberg JR and Wolffe AP: Precipitous release of methyl-CpG binding protein 2 and histone deacetylase 1 from the methylated human multidrug resistance gene (MDR1) on activation. Mol Cell Biol. 22:1844–1857. 2002. View Article : Google Scholar : PubMed/NCBI

25 

Wada R, Akiyama Y, Hashimoto Y, Fukamachi H and Yuasa Y: miR-212 is downregulated and suppresses methyl-CpG-binding protein MeCP2 in human gastric cancer. Int J Cancer. 127:1106–1114. 2010. View Article : Google Scholar

26 

Tong D, Zhao L, He K, Sun H, Cai D, Ni L, Sun R, Chang S, Song T and Huang C: MECP2 promotes the growth of gastric cancer cells by suppressing miR-338-mediated antiproliferative effect. Oncotarget. 7:34845–34859. 2016. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Zhu F, Wu Q, Ni Z, Lei C, Li T and Shi Y: miR-19a/b and MeCP2 repress reciprocally to regulate multidrug resistance in gastric cancer cells. Int J Mol Med 42: 228-236, 2018.
APA
Zhu, F., Wu, Q., Ni, Z., Lei, C., Li, T., & Shi, Y. (2018). miR-19a/b and MeCP2 repress reciprocally to regulate multidrug resistance in gastric cancer cells. International Journal of Molecular Medicine, 42, 228-236. https://doi.org/10.3892/ijmm.2018.3581
MLA
Zhu, F., Wu, Q., Ni, Z., Lei, C., Li, T., Shi, Y."miR-19a/b and MeCP2 repress reciprocally to regulate multidrug resistance in gastric cancer cells". International Journal of Molecular Medicine 42.1 (2018): 228-236.
Chicago
Zhu, F., Wu, Q., Ni, Z., Lei, C., Li, T., Shi, Y."miR-19a/b and MeCP2 repress reciprocally to regulate multidrug resistance in gastric cancer cells". International Journal of Molecular Medicine 42, no. 1 (2018): 228-236. https://doi.org/10.3892/ijmm.2018.3581
Copy and paste a formatted citation
x
Spandidos Publications style
Zhu F, Wu Q, Ni Z, Lei C, Li T and Shi Y: miR-19a/b and MeCP2 repress reciprocally to regulate multidrug resistance in gastric cancer cells. Int J Mol Med 42: 228-236, 2018.
APA
Zhu, F., Wu, Q., Ni, Z., Lei, C., Li, T., & Shi, Y. (2018). miR-19a/b and MeCP2 repress reciprocally to regulate multidrug resistance in gastric cancer cells. International Journal of Molecular Medicine, 42, 228-236. https://doi.org/10.3892/ijmm.2018.3581
MLA
Zhu, F., Wu, Q., Ni, Z., Lei, C., Li, T., Shi, Y."miR-19a/b and MeCP2 repress reciprocally to regulate multidrug resistance in gastric cancer cells". International Journal of Molecular Medicine 42.1 (2018): 228-236.
Chicago
Zhu, F., Wu, Q., Ni, Z., Lei, C., Li, T., Shi, Y."miR-19a/b and MeCP2 repress reciprocally to regulate multidrug resistance in gastric cancer cells". International Journal of Molecular Medicine 42, no. 1 (2018): 228-236. https://doi.org/10.3892/ijmm.2018.3581
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